##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1439764
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281014110645913	32.0	32.0	32.0	32.0	32.0
2	31.358082296820868	32.0	32.0	32.0	32.0	32.0
3	31.445526489063486	32.0	32.0	32.0	32.0	32.0
4	31.531325272753033	32.0	32.0	32.0	32.0	32.0
5	31.481504607699595	32.0	32.0	32.0	32.0	32.0
6	35.05067288805665	36.0	36.0	36.0	36.0	36.0
7	35.06992673799317	36.0	36.0	36.0	36.0	36.0
8	35.005632173050586	36.0	36.0	36.0	36.0	36.0
9	35.1429449548676	36.0	36.0	36.0	36.0	36.0
10	34.972312128932245	36.0	36.0	36.0	36.0	36.0
11	35.137528094882214	36.0	36.0	36.0	36.0	36.0
12	35.043890526502956	36.0	36.0	36.0	36.0	36.0
13	35.08189744985984	36.0	36.0	36.0	36.0	36.0
14	35.01920800909038	36.0	36.0	36.0	36.0	36.0
15	34.9951242009107	36.0	36.0	36.0	36.0	36.0
16	35.00578289219622	36.0	36.0	36.0	36.0	36.0
17	34.97298029399263	36.0	36.0	36.0	36.0	36.0
18	34.96931719365118	36.0	36.0	36.0	36.0	36.0
19	34.968448301249374	36.0	36.0	36.0	36.0	36.0
20	34.96468796274945	36.0	36.0	36.0	36.0	36.0
21	34.960251819048125	36.0	36.0	36.0	36.0	36.0
22	34.947455277392685	36.0	36.0	36.0	36.0	36.0
23	34.88504574360798	36.0	36.0	36.0	32.0	36.0
24	34.86344706493564	36.0	36.0	36.0	32.0	36.0
25	34.83855965283199	36.0	36.0	36.0	32.0	36.0
26	34.77835811980297	36.0	36.0	36.0	32.0	36.0
27	34.77188761491467	36.0	36.0	36.0	32.0	36.0
28	34.75041465129007	36.0	36.0	36.0	32.0	36.0
29	34.71473658182869	36.0	36.0	36.0	32.0	36.0
30	34.70600251152272	36.0	36.0	36.0	32.0	36.0
31	34.69079585265363	36.0	36.0	36.0	32.0	36.0
32	34.65089903623094	36.0	36.0	36.0	32.0	36.0
33	34.626315840651664	36.0	36.0	36.0	32.0	36.0
34	34.606388269188564	36.0	36.0	36.0	32.0	36.0
35	34.561982380445684	36.0	36.0	36.0	32.0	36.0
36	34.548726735770586	36.0	36.0	36.0	32.0	36.0
37	34.530811994187935	36.0	36.0	36.0	32.0	36.0
38	34.50770473494267	36.0	36.0	36.0	32.0	36.0
39	34.50693030246624	36.0	36.0	36.0	32.0	36.0
40	34.48259298051625	36.0	36.0	36.0	32.0	36.0
41	34.47635237441692	36.0	36.0	36.0	32.0	36.0
42	34.41289127940412	36.0	36.0	36.0	32.0	36.0
43	34.4037640891146	36.0	36.0	36.0	32.0	36.0
44	34.34898288886234	36.0	36.0	36.0	32.0	36.0
45	34.31924884911694	36.0	36.0	36.0	32.0	36.0
46	34.29651317854871	36.0	36.0	36.0	32.0	36.0
47	34.27033110982077	36.0	36.0	36.0	32.0	36.0
48	34.2319817692344	36.0	36.0	36.0	32.0	36.0
49	34.20481828966414	36.0	36.0	36.0	32.0	36.0
50	33.59537326950806	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	18.0
21	38.0
22	131.0
23	448.0
24	1225.0
25	2906.0
26	5741.0
27	10385.0
28	16990.0
29	26190.0
30	37251.0
31	53448.0
32	75929.0
33	122466.0
34	253064.0
35	833530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15482748824186	17.427261379746756	11.572121105192197	26.845790026819188
2	16.140129591673016	19.8468765128463	36.897162221575506	27.115831673905177
3	18.386719471690462	23.74204903310408	28.495126277815363	29.376105217390098
4	12.236449862616373	15.633117649837056	35.365865516848594	36.76456697069798
5	14.204063999377675	36.89861671773985	33.569043259867584	15.328276023014883
6	35.27844918597544	35.22330110573984	16.226523865088627	13.271725843196089
7	30.727904523174992	30.11085852324306	20.414679360422515	18.74655759315943
8	28.450773876829814	33.23586365543242	18.74876715906218	19.56459530867559
9	27.5890019336771	13.710215149359886	17.79128789562589	40.90949502133713
10	15.430601155900742	27.05253645963203	32.146153862182345	25.37070852228488
11	37.83411725810619	20.611850275461812	22.011732478378402	19.542299988053596
12	24.748169658949866	23.308970459639426	28.877471854664567	23.06538802674614
13	30.021566169244075	19.26313852009543	24.793940635733165	25.921354674927333
14	23.526351614157914	19.075262543757294	25.201353003278328	32.197032838806464
15	25.310550809196357	27.05334444675974	22.014794748906024	25.621309995137874
16	25.87674578192416	25.573083216878416	23.521068847776984	25.02910215342044
17	24.362812238672447	25.70400426736604	24.972287124834345	24.960896369127163
18	24.958135470907365	25.04078796301891	25.671030579338407	24.330045986735314
19	25.828000858474624	24.7544213874089	25.041204698273255	24.376373055843217
20	26.00053481321475	24.233914798003827	24.878607818691375	24.88694257009005
21	26.6835398023565	23.95566217796806	24.48616578828197	24.874632231393477
22	26.0381048825397	24.17675686901247	24.686076805696494	25.099061442751342
23	24.844869762835142	24.141209842209594	25.185065823562013	25.82885457139325
24	25.105208726194768	24.59783143961099	24.99532907289216	25.30163076130208
25	25.26068961110312	24.279678469500375	25.07440584717091	25.385226072225592
26	25.03884535692927	24.891521258499267	25.243751575867744	24.82588180870372
27	25.459650117178654	24.711707168666415	24.704066592483617	25.124576121671318
28	24.907983471407327	24.57367469456903	25.175819466314554	25.34252236770909
29	24.933338056450978	24.731008031383727	25.106426426676727	25.229227485488572
30	25.021653621242567	24.785912192221645	25.231000900873994	24.961433285661794
31	25.303201642637447	24.47528403012984	24.708467973848943	25.51304635338377
32	25.099325144681313	24.69660683079163	24.633469378683017	25.57059864584404
33	24.892790015683843	24.417412540685614	25.009689532110208	25.680107911520334
34	25.265590390333653	24.48328386759342	25.143344143328168	25.107781598744754
35	25.658201357348204	24.376701286848338	25.113164443894952	24.851932911908502
36	24.982634837017525	24.78932584269663	24.897753920359193	25.330285399926648
37	25.513453741757896	24.71336261235762	24.635083013188066	25.138100632696425
38	25.03988880453062	24.584076291983965	25.08837358565795	25.287661317827464
39	25.452440207738736	24.47591257302772	24.68463762760533	25.387009591628217
40	25.579101637112515	24.609058642939996	25.103804185507006	24.708035534440487
41	25.020907075343263	24.668265143390585	25.2465784633508	25.064249317915348
42	25.705458705305205	24.893227026880606	24.760633365677204	24.64068090213699
43	25.200486752524405	24.347766897772246	24.928772158422387	25.522974191280962
44	24.869299590274096	24.848323624424637	24.721356519878896	25.561020265422368
45	24.993193234352773	24.82795552542674	24.90748355263158	25.271367687588903
46	24.90277805753372	24.725038496346926	24.83123771051442	25.540945735604936
47	25.136761183994842	24.556585373985232	25.166627770646844	25.140025671373085
48	25.53143165134333	25.05224890831211	24.381226371651053	25.03509306869351
49	24.976315431225846	24.97791292413437	24.59180583376628	25.45396581087351
50	24.980083361636837	25.144625702638855	24.68218462775855	25.193106307965753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	14.5
2	22.0
3	76.0
4	130.0
5	164.0
6	198.0
7	186.0
8	174.0
9	238.5
10	303.0
11	479.0
12	655.0
13	1214.0
14	1773.0
15	2320.0
16	2867.0
17	3208.0
18	3549.0
19	3683.0
20	3817.0
21	4186.5
22	4556.0
23	5362.5
24	6169.0
25	7177.0
26	8185.0
27	10037.5
28	11890.0
29	13993.0
30	16096.0
31	18584.0
32	21072.0
33	24607.0
34	28142.0
35	32682.5
36	37223.0
37	43041.0
38	48859.0
39	53532.0
40	58205.0
41	63926.0
42	69647.0
43	73034.5
44	76422.0
45	84008.0
46	91594.0
47	97518.0
48	103442.0
49	107550.5
50	111659.0
51	111847.5
52	112036.0
53	110840.0
54	109644.0
55	109074.0
56	108504.0
57	105082.5
58	101661.0
59	93968.0
60	86275.0
61	75622.0
62	64969.0
63	56369.5
64	47770.0
65	40529.0
66	33288.0
67	28362.0
68	23436.0
69	20559.0
70	17682.0
71	13827.5
72	9973.0
73	8421.5
74	6870.0
75	5430.0
76	3990.0
77	3407.0
78	2824.0
79	2322.0
80	1820.0
81	1433.0
82	1046.0
83	847.5
84	649.0
85	484.0
86	319.0
87	236.5
88	154.0
89	115.0
90	76.0
91	61.5
92	47.0
93	36.5
94	26.0
95	24.0
96	22.0
97	20.0
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008543066780389009
2	7.640141023112122E-4
3	1.3891165496567493E-4
4	0.0
5	0.0
6	2.7782330993134986E-4
7	6.945582748283746E-5
8	0.0
9	0.0013891165496567493
10	0.0014585723771395867
11	0.0
12	3.472791374141873E-4
13	6.251024473455371E-4
14	2.7782330993134986E-4
15	0.004445172958901598
16	5.556466198626997E-4
17	0.0
18	6.945582748283746E-5
19	6.945582748283746E-5
20	6.251024473455371E-4
21	0.0
22	6.945582748283746E-5
23	4.167349648970248E-4
24	4.8619079237986225E-4
25	0.0017363956870709366
26	0.005625922026109835
27	0.005695377853592672
28	0.0053480987161784845
29	0.0025698656168649863
30	0.0035422472016247105
31	0.008890345917803195
32	0.0033338797191761983
33	0.0045840846138672725
34	0.003264423891693361
35	0.003125512236727686
36	0.006945582748283746
37	0.0036811588565903858
38	0.009515448365148733
39	0.00493136375128146
40	0.0023614981344164737
41	0.004167349648970248
42	0.0018753073420366116
43	0.0012502048946910743
44	0.0014585723771395867
45	0.0013891165496567493
46	0.0011807490672082368
47	0.001944763169519449
48	0.0014585723771395867
49	5.556466198626997E-4
50	9.02925757276887E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1439764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.88481981873305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17896746679158	48.207971676489315
2	12.44892756311675	15.15901423233646
3	3.8376918778113187	7.009715355010723
4	1.5513429923677262	3.778129542694527
5	0.8210367857422485	2.4994338382234265
6	0.48601111486081433	1.7754419494921352
7	0.3226855615743371	1.3752656592192143
8	0.2196945999576678	1.0700852906856995
9	0.17345756617479716	0.950483939647364
>10	0.85957079298609	9.22274914477903
>50	0.05421903703543879	2.288416545476698
>100	0.04294099971697963	5.1911620132668945
>500	0.003453641864139476	1.4721308126785742
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3891165496567493E-4
2	0.0	0.0	0.0	0.0	1.3891165496567493E-4
3	0.0	0.0	0.0	0.0	1.3891165496567493E-4
4	0.0	0.0	0.0	0.0	1.3891165496567493E-4
5	0.0	0.0	0.0	0.0	1.3891165496567493E-4
6	0.0	0.0	0.0	0.0	2.7782330993134986E-4
7	0.0	0.0	0.0	0.0	2.7782330993134986E-4
8	0.0	0.0	0.0	0.0	2.7782330993134986E-4
9	0.0	0.0	0.0	0.0	2.7782330993134986E-4
10	0.0	0.0	0.0	0.0	2.7782330993134986E-4
11	0.0	0.0	0.0	0.0	2.7782330993134986E-4
12	0.0	0.0	0.0	0.0	8.334699297940496E-4
13	0.0	0.0	0.0	0.0	9.029257572768871E-4
14	0.0	0.0	0.0	0.0	9.723815847597245E-4
15	0.0	0.0	0.0	0.0	0.0013196607221739119
16	0.0	0.0	0.0	2.083674824485124E-4	0.0013891165496567493
17	0.0	0.0	0.0	2.083674824485124E-4	0.0013891165496567493
18	0.0	0.0	0.0	2.7782330993134986E-4	0.0013891165496567493
19	0.0	0.0	0.0	2.7782330993134986E-4	0.0013891165496567493
20	0.0	0.0	0.0	4.167349648970248E-4	0.0015280282046224243
21	0.0	0.0	0.0	4.167349648970248E-4	0.0015974840321052617
22	0.0	0.0	0.0	6.945582748283746E-4	0.0015974840321052617
23	0.0	0.0	0.0	0.0011112932397253994	0.0016669398595880991
24	0.0	0.0	0.0	0.0016669398595880991	0.0016669398595880991
25	0.0	0.0	0.0	0.002083674824485124	0.0016669398595880991
26	0.0	0.0	0.0	0.002500409789382149	0.0016669398595880991
27	0.0	0.0	0.0	0.0031949680642105235	0.0018058515145537742
28	0.0	0.0	0.0	0.00493136375128146	0.0018058515145537742
29	0.0	0.0	0.0	0.009376536710183058	0.0018058515145537742
30	0.0	0.0	0.0	0.016599942768398154	0.0018058515145537742
31	0.0	0.0	0.0	0.033824987984141845	0.0018058515145537742
32	0.0	0.0	0.0	0.05334207550681917	0.0018058515145537742
33	0.0	0.0	0.0	0.07140059065235692	0.0018058515145537742
34	0.0	0.0	0.0	0.09550176278890152	0.0018753073420366116
35	0.0	0.0	0.0	0.1221728005423111	0.0018753073420366116
36	0.0	0.0	0.0	0.16141534307011426	0.0018753073420366116
37	0.0	0.0	0.0	0.2182302099510753	0.0018753073420366116
38	0.0	0.0	0.0	0.29692366248913016	0.0018753073420366116
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	45	0.001398202	24.444868	7
GTATCAA	2215	0.0	21.850637	1
CGTATAT	205	0.0	20.390596	10
GTCCTAT	340	0.0	18.76438	1
ACCGTAT	60	0.0074120285	18.333652	8
TAGCGTA	235	0.0	17.787542	7
TAACGGC	140	8.3702616E-8	17.286615	36
TTAACGG	140	8.3702616E-8	17.286615	35
AGCGTAT	245	0.0	17.06152	8
TAGGACG	245	0.0	17.06152	4
GTATTAC	155	1.439912E-8	17.031961	1
AACGCAG	2810	0.0	16.989618	6
ATAGCGT	260	0.0	16.92337	6
GTATTAG	340	0.0	16.823236	1
AAATTCG	80	0.0019888303	16.499712	21
GATATAA	190	6.166374E-10	16.210245	1
CGTTTAT	340	0.0	16.17675	31
GTACCGT	110	5.621604E-5	16.000278	6
TTAGACC	110	5.621604E-5	16.000278	4
GGTATAT	110	5.623274E-5	15.999723	1
>>END_MODULE