FastQCFastQC Report
Thu 2 Feb 2017
SRR4064052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865092
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11520.1331650275346437No Hit
GGGTAGGCACACGCTGAGCCAGTCA10960.12669172758504296No Hit
GATTAAGAGGGACGGCCGGGGGCAT10360.11975604906761361No Hit
GAATAGGACCGCGGTTCTATTTTGT10300.11906248121587068No Hit
GCGCAAGACGGACCAGAGCGAAAGC10210.11802212943825628No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10150.11732856158651335No Hit
GTATCTGATCGTCTTCGAACCTCCG9680.11189561341452701No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9490.10969931521734105No Hit
GTACATGGGGAATAATTGCAATCCC9460.10935253129146959No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC9410.10877455808168379No Hit
TCGTAGTTCCGACCATAAACGATGC9250.1069250438103693No Hit
GTATCAACGCAGAGTACATGGGGAA9070.1048443402551405No Hit
ATCAGATACCGTCGTAGTTCCGACC9000.1040351777614404No Hit
GTCCTATTCCATTATTCCTAGCTGC8930.10322601526774032No Hit
GAACTACGACGGTATCTGATCGTCT8920.10311042062578314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.015.5658391
TCGAACG551.1299213E-515.5414323
CTAACAC456.771307E-414.7739543
CGAACGT602.570526E-514.2471374
CGGTCCA1900.014.00042510
CGCCGGT1900.013.9996177
CGAACGA1101.8553692E-1013.82020116
CACCGCA500.001498917913.2996357
GGGCCGT500.001500225413.2980986
CGTTTCC655.439565E-513.15576714
ATAATTG2200.012.52383212
GCCGGTC2050.012.5118538
CCGGTCC2150.012.372479
GTCCTAT2150.012.3717551
TTCGGAA1551.8189894E-1212.25985618
AATTGCA2350.012.12943214
CAATCCC2350.012.12943219
ATCTAGG550.003067182112.0905771
GTCTATC550.003067182112.0905771
TCAGGAC1103.8184226E-812.0877813
Produced by FastQC (version 0.11.5)