##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064052_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865092
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255250308637695	32.0	32.0	32.0	32.0	32.0
2	30.762046117638356	32.0	32.0	32.0	32.0	32.0
3	30.78586092577437	32.0	32.0	32.0	32.0	32.0
4	30.821575046353452	32.0	32.0	32.0	32.0	32.0
5	30.67550272109787	32.0	32.0	32.0	32.0	32.0
6	34.38330027326573	36.0	36.0	36.0	32.0	36.0
7	34.31265691972646	36.0	36.0	36.0	32.0	36.0
8	34.259593199336024	36.0	36.0	36.0	32.0	36.0
9	34.45780795568564	36.0	36.0	36.0	32.0	36.0
10	34.06768644259801	36.0	36.0	36.0	32.0	36.0
11	34.4325447466859	36.0	36.0	36.0	32.0	36.0
12	34.1896029555238	36.0	36.0	36.0	32.0	36.0
13	34.30473984269881	36.0	36.0	36.0	32.0	36.0
14	34.183175893430985	36.0	36.0	36.0	32.0	36.0
15	34.097191974957575	36.0	36.0	36.0	32.0	36.0
16	34.102329000846154	36.0	36.0	36.0	32.0	36.0
17	34.015179888381816	36.0	36.0	36.0	32.0	36.0
18	34.010916757986436	36.0	36.0	36.0	32.0	36.0
19	34.04653146717343	36.0	36.0	36.0	32.0	36.0
20	34.03488761888909	36.0	36.0	36.0	32.0	36.0
21	34.009210581071144	36.0	36.0	36.0	32.0	36.0
22	33.98798624886139	36.0	36.0	36.0	32.0	36.0
23	33.918048022638054	36.0	36.0	36.0	32.0	36.0
24	33.899446532854306	36.0	36.0	36.0	32.0	36.0
25	33.494489603417904	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	6.0
5	13.0
6	33.0
7	12.0
8	50.0
9	68.0
10	79.0
11	29.0
12	50.0
13	50.0
14	71.0
15	138.0
16	231.0
17	288.0
18	389.0
19	564.0
20	831.0
21	1292.0
22	2128.0
23	3226.0
24	4910.0
25	7238.0
26	10301.0
27	13494.0
28	18460.0
29	24870.0
30	32774.0
31	44687.0
32	63372.0
33	87765.0
34	181765.0
35	365904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53053176911379	17.88390931535522	11.953443460864637	25.632115454666348
2	16.64215924276684	19.416122185692288	37.73530594975515	26.206412621785713
3	18.604586643732294	23.56876902287311	28.877198214725396	28.949446118669204
4	12.413691057624838	15.348975390174797	36.47604041795896	35.7612931342414
5	14.531537926203034	36.665079038452205	33.749893066303514	15.05348996904125
6	34.17320831936368	36.122986924402	16.549822537197823	13.153982219036497
7	30.075859971811212	30.457992583968007	20.933236056206447	18.532911388014334
8	28.070335708925064	33.2887355784853	19.178229511533164	19.46269920105647
9	26.935807700670328	14.004709409103317	18.956296868566778	40.10318602165958
10	16.006666890284315	26.62522800325248	31.35323761726914	26.01486748919406
11	37.528857408546884	21.040925472706327	22.47766586937714	18.952551249369648
12	24.23189834060893	23.836078164652864	28.980352421073086	22.95167107366512
13	29.149865008363236	19.41901542860712	25.49132560167866	25.939793961350983
14	23.53712805718113	19.791875125075045	24.835942027644332	31.83505479009949
15	25.176075683916245	27.640846088627168	22.008621896192555	25.174456331264032
16	25.728450699011923	25.673506025406418	24.01278880655542	24.58525446902624
17	23.8277169933756	26.074137306817224	25.2002530851988	24.897892614608384
18	24.78971424737309	25.06330841871824	26.19493555787182	23.952041776036854
19	25.654868771747985	24.965758495594876	24.93440901014289	24.444963722514252
20	25.848083107748547	24.427477383679232	24.69146942458527	25.03297008398695
21	27.093735105895632	24.248874410598358	24.108320338347777	24.54907014515823
22	25.972516088580605	24.383266256609822	25.043699164654747	24.60051849015482
23	24.638122122214767	24.209144126425876	25.358522871885054	25.794210879474306
24	24.87503369260544	25.09367411933603	25.166901309875954	24.864390878182576
25	24.92306873605982	24.520481069124763	25.262029041841355	25.294421152974056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	14.0
2	77.5
3	141.0
4	141.0
5	141.0
6	438.5
7	736.0
8	736.0
9	736.0
10	857.5
11	979.0
12	979.0
13	979.0
14	1403.0
15	1827.0
16	1827.0
17	1827.0
18	3405.0
19	4983.0
20	4983.0
21	4983.0
22	8372.0
23	11761.0
24	11761.0
25	11761.0
26	18608.5
27	25456.0
28	25456.0
29	25456.0
30	32391.5
31	39327.0
32	39327.0
33	39327.0
34	48236.0
35	57145.0
36	57145.0
37	57145.0
38	65448.5
39	73752.0
40	73752.0
41	73752.0
42	83684.0
43	93616.0
44	93616.0
45	93616.0
46	102792.5
47	111969.0
48	111969.0
49	111969.0
50	114207.0
51	116445.0
52	116445.0
53	116445.0
54	107071.5
55	97698.0
56	97698.0
57	97698.0
58	89812.5
59	81927.0
60	81927.0
61	81927.0
62	72404.5
63	62882.0
64	62882.0
65	62882.0
66	51896.0
67	40910.0
68	40910.0
69	40910.0
70	30716.0
71	20522.0
72	20522.0
73	20522.0
74	15925.0
75	11328.0
76	11328.0
77	11328.0
78	8992.0
79	6656.0
80	6656.0
81	6656.0
82	4520.0
83	2384.0
84	2384.0
85	2384.0
86	1864.5
87	1345.0
88	1345.0
89	1345.0
90	941.5
91	538.0
92	538.0
93	538.0
94	390.5
95	243.0
96	243.0
97	243.0
98	375.5
99	508.0
100	508.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03895539433956158
2	0.005548542813943488
3	0.0017339196293573401
4	0.003583433900671836
5	0.00809162493700092
6	0.014102546318773033
7	0.024159280169045606
8	0.0375682586360757
9	0.05086164246114864
10	0.06022480845967828
11	0.05733494241074938
12	0.06450181021209304
13	0.06866321732255067
14	0.07062832623582233
15	0.06346145843447865
16	0.06773846018689342
17	0.06484859413796452
18	0.07779519403716599
19	0.07444294942040847
20	0.07767959939520883
21	0.07583008512389433
22	0.07617686904976581
23	0.08230338507349508
24	0.07617686904976581
25	0.0790667350986947
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	865092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.027263706132096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57364568696417	35.33540883229357
2	14.493823441690312	13.921973610883596
3	4.997001949123406	7.199769910518176
4	2.2426923721699943	4.308415118797571
5	1.2158588529949297	2.9197186881111397
6	0.7753795564735546	2.234361505865949
7	0.49346971488674646	1.6590000089498913
8	0.34241175459484996	1.3156079707204997
9	0.26078183234981406	1.1272174048829564
>10	1.3806867017517124	11.98453402776788
>50	0.10958480371565178	3.7013245238388093
>100	0.10280995073308169	10.04080257539285
>500	0.010401947953621878	3.514118247871165
>1k	0.0014514345981797969	0.7377475741059243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1152	0.1331650275346437	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1096	0.12669172758504296	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1036	0.11975604906761361	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1030	0.11906248121587068	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1021	0.11802212943825628	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1015	0.11732856158651335	No Hit
GTATCTGATCGTCTTCGAACCTCCG	968	0.11189561341452701	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	949	0.10969931521734105	No Hit
GTACATGGGGAATAATTGCAATCCC	946	0.10935253129146959	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	941	0.10877455808168379	No Hit
TCGTAGTTCCGACCATAAACGATGC	925	0.1069250438103693	No Hit
GTATCAACGCAGAGTACATGGGGAA	907	0.1048443402551405	No Hit
ATCAGATACCGTCGTAGTTCCGACC	900	0.1040351777614404	No Hit
GTCCTATTCCATTATTCCTAGCTGC	893	0.10322601526774032	No Hit
GAACTACGACGGTATCTGATCGTCT	892	0.10311042062578314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.15594641957156E-4	0.0
5	0.0	0.0	0.0	1.15594641957156E-4	0.0
6	0.0	0.0	0.0	1.15594641957156E-4	0.0
7	0.0	0.0	0.0	1.15594641957156E-4	0.0
8	0.0	0.0	0.0	1.15594641957156E-4	0.0
9	0.0	0.0	0.0	3.46783925871468E-4	0.0
10	0.0	0.0	0.0	3.46783925871468E-4	0.0
11	0.0	0.0	0.0	3.46783925871468E-4	0.0
12	0.0	0.0	0.0	3.46783925871468E-4	2.31189283914312E-4
13	0.0	0.0	0.0	4.62378567828624E-4	3.46783925871468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	415	0.0	15.565839	1
TCGAACG	55	1.1299213E-5	15.541432	3
CTAACAC	45	6.771307E-4	14.773954	3
CGAACGT	60	2.570526E-5	14.247137	4
CGGTCCA	190	0.0	14.000425	10
CGCCGGT	190	0.0	13.999617	7
CGAACGA	110	1.8553692E-10	13.820201	16
CACCGCA	50	0.0014989179	13.299635	7
GGGCCGT	50	0.0015002254	13.298098	6
CGTTTCC	65	5.439565E-5	13.155767	14
ATAATTG	220	0.0	12.523832	12
GCCGGTC	205	0.0	12.511853	8
CCGGTCC	215	0.0	12.37247	9
GTCCTAT	215	0.0	12.371755	1
TTCGGAA	155	1.8189894E-12	12.259856	18
AATTGCA	235	0.0	12.129432	14
CAATCCC	235	0.0	12.129432	19
ATCTAGG	55	0.0030671821	12.090577	1
GTCTATC	55	0.0030671821	12.090577	1
TCAGGAC	110	3.8184226E-8	12.087781	3
>>END_MODULE