FastQCFastQC Report
Thu 2 Feb 2017
SRR4064052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865092
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11890.13744202928705848No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11780.13617048822552977No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10880.12576697044938573No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10200.11790653479629913No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9830.11362953304388435No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9770.11293596519214141No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9310.10761861166211223No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9280.10727182773624076No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9240.10680944916841215No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8890.10276363669991168No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC8770.10137650099642581No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8680.10033614921881141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11350.025.7808881
ATTTCGT1051.1277734E-1025.14107542
GTCCTAA1900.024.3168751
GGTATCA5100.023.726551
CTAGGAC1105.2496034E-921.9997123
GATCGTA602.872119E-421.9997112
GTCTTAG1451.2732926E-1121.2423271
GATTTCG1259.968062E-1021.118541
ATAATAC755.4708835E-520.5330643
TATATGC755.4708835E-520.5330644
TCTATAC755.4708835E-520.5330643
GTATAAA1101.2639975E-720.0008931
GGCGTTA3300.019.9985842
GTAACGG550.004484439319.9985816
ATAGGGG808.985713E-519.2497463
CGCAATA2750.019.19974736
ATACACT3000.019.0664164
CGTGATT708.1246486E-418.85580428
CGAATGC2700.018.7394143
CATTAGG600.007408533718.3341521
Produced by FastQC (version 0.11.5)