##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865092
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366472005289612	32.0	32.0	32.0	32.0	32.0
2	31.414680750717842	32.0	32.0	32.0	32.0	32.0
3	31.497933167801808	32.0	32.0	32.0	32.0	32.0
4	31.589339630929427	32.0	32.0	32.0	32.0	32.0
5	31.5270919162355	32.0	32.0	32.0	32.0	32.0
6	35.13523648351852	36.0	36.0	36.0	36.0	36.0
7	35.15608744503475	36.0	36.0	36.0	36.0	36.0
8	35.10001364016775	36.0	36.0	36.0	36.0	36.0
9	35.20776171782885	36.0	36.0	36.0	36.0	36.0
10	35.05944685651931	36.0	36.0	36.0	36.0	36.0
11	35.207303963046705	36.0	36.0	36.0	36.0	36.0
12	35.10737701886042	36.0	36.0	36.0	36.0	36.0
13	35.158393558141796	36.0	36.0	36.0	36.0	36.0
14	35.09411715748151	36.0	36.0	36.0	36.0	36.0
15	35.06712580858452	36.0	36.0	36.0	36.0	36.0
16	35.07524980002127	36.0	36.0	36.0	36.0	36.0
17	35.04700887304472	36.0	36.0	36.0	36.0	36.0
18	35.043173442824575	36.0	36.0	36.0	36.0	36.0
19	35.04656614556602	36.0	36.0	36.0	36.0	36.0
20	35.0332877890444	36.0	36.0	36.0	36.0	36.0
21	35.02459507196922	36.0	36.0	36.0	36.0	36.0
22	35.01554747934324	36.0	36.0	36.0	36.0	36.0
23	34.96764968350187	36.0	36.0	36.0	36.0	36.0
24	34.9357756169286	36.0	36.0	36.0	36.0	36.0
25	34.91104645517471	36.0	36.0	36.0	32.0	36.0
26	34.846768898568016	36.0	36.0	36.0	32.0	36.0
27	34.850380075182755	36.0	36.0	36.0	32.0	36.0
28	34.8306087676224	36.0	36.0	36.0	32.0	36.0
29	34.79530038423659	36.0	36.0	36.0	32.0	36.0
30	34.780857989670466	36.0	36.0	36.0	32.0	36.0
31	34.78040485867399	36.0	36.0	36.0	32.0	36.0
32	34.74344809569387	36.0	36.0	36.0	32.0	36.0
33	34.71753986859201	36.0	36.0	36.0	32.0	36.0
34	34.712595885755505	36.0	36.0	36.0	32.0	36.0
35	34.67674998728459	36.0	36.0	36.0	32.0	36.0
36	34.644137270949216	36.0	36.0	36.0	32.0	36.0
37	34.64433840562622	36.0	36.0	36.0	32.0	36.0
38	34.60511598766374	36.0	36.0	36.0	32.0	36.0
39	34.61053968826437	36.0	36.0	36.0	32.0	36.0
40	34.58827847211626	36.0	36.0	36.0	32.0	36.0
41	34.57712243322098	36.0	36.0	36.0	32.0	36.0
42	34.510210474724076	36.0	36.0	36.0	32.0	36.0
43	34.514533714333275	36.0	36.0	36.0	32.0	36.0
44	34.4408606252283	36.0	36.0	36.0	32.0	36.0
45	34.43017158868652	36.0	36.0	36.0	32.0	36.0
46	34.40746186532762	36.0	36.0	36.0	32.0	36.0
47	34.39889514641217	36.0	36.0	36.0	32.0	36.0
48	34.348497038465275	36.0	36.0	36.0	32.0	36.0
49	34.3469214834954	36.0	36.0	36.0	32.0	36.0
50	33.741189376390025	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	0.0
20	7.0
21	18.0
22	72.0
23	247.0
24	576.0
25	1403.0
26	2881.0
27	5318.0
28	8967.0
29	13707.0
30	20381.0
31	29081.0
32	43227.0
33	70963.0
34	150736.0
35	517504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68976439851102	18.894707636817646	12.463411250606923	25.952116714064417
2	15.809544726818753	20.24286630793506	37.34765947854835	26.59992948669784
3	17.87034136451016	24.199184590682787	29.290580022587324	28.639894022219732
4	12.120213803849763	15.999454393289964	36.46664169822401	35.41369010463627
5	14.140230172051066	37.05802388647682	33.68138880026633	15.12035714120579
6	34.36212564675202	36.549291867223374	16.180244413310955	12.908338072713654
7	29.875504282791386	30.749286201435687	20.951924077263637	18.42328543850929
8	28.02615213179639	33.55724015480435	19.00283438062079	19.41377333277848
9	27.025202468228148	13.954413148470534	18.734351049736787	40.286033333564525
10	15.995843163819343	26.863380423336082	31.345041678178116	25.795734734666464
11	37.82938693225692	20.924595303158508	22.34132323498541	18.904694529599166
12	24.199128644567207	23.87118550808067	28.932052077878694	22.99763376947343
13	29.256017024877266	19.433999123787547	25.56979818214815	25.74018566918703
14	23.487124475922187	19.841843228053467	24.827677088306316	31.843355207718034
15	25.17712910396776	27.68785973478898	22.002041507571267	25.132969653672
16	25.744291023425443	25.816653854823514	23.84748319529295	24.591571926458094
17	23.900926144271363	26.122770757329857	25.11675058837673	24.859552510022056
18	24.944312216865047	24.97459804806662	26.077603396636885	24.003486338431447
19	25.627447716543443	25.027858308711675	24.92289837381458	24.421795600930306
20	25.964354988983757	24.37931026510659	24.609345198049674	25.046989547859983
21	27.302182889218717	24.34561873188054	23.990511991788157	24.361686387112584
22	26.064425592220935	24.462397597478184	24.924083131138804	24.549093679162077
23	24.675377219011864	24.202704717824112	25.321675080137005	25.800242983027022
24	24.941768862553708	25.119670044746943	24.99945092227718	24.939110170422165
25	24.84515847125924	24.593736056170656	25.396900626301903	25.164204846268195
26	24.56022519059702	25.61936084249952	25.624447283089317	24.195966683814138
27	25.25761993170211	24.84538180002173	24.944799857577852	24.95219841069831
28	24.472550427196296	24.77472634814504	25.741365617836472	25.011357606822195
29	24.61373068075416	24.594425884610494	25.478978579768114	25.312864854867236
30	24.450039419139898	25.136117411237574	25.733415639572655	24.68042753004987
31	25.362731826415867	24.7113115915809	24.635245129376568	25.290711452626663
32	25.012686966583857	24.91824275975914	24.440357385201736	25.62871288845526
33	24.614445588882994	24.609127995625702	25.199265247331663	25.57716116815964
34	25.36326708019966	24.760191707915926	25.494239745869102	24.38230146601531
35	25.82182255776774	24.504571364496524	25.461847111006303	24.211758966729438
36	24.457348568574773	25.247742227853486	25.014797482578278	25.280111720993464
37	25.70038413476044	24.84125396937326	24.714095306573753	24.744266589292547
38	24.92338354009084	24.66258122616393	24.967428611724777	25.446606622020457
39	25.73157620946766	24.474539043985896	24.38991965782325	25.403965088723197
40	25.82470109389865	24.775423057760577	25.07008095289288	24.329794895447886
41	24.64326173907012	25.147966615032484	25.757057313943545	24.451714331953852
42	26.05592611002578	25.331129272775616	24.948732472516674	23.664212144681933
43	25.108140537616265	24.230763006341625	25.35054642471546	25.310550031326656
44	24.658181902251815	24.9812734082397	24.909141351088916	25.45140333841957
45	24.86654444318573	25.279106489327024	24.856025209170923	24.998323858316322
46	24.728350079298657	24.82371654082147	24.930642573437957	25.517290806441917
47	25.14819575711617	24.720138901733037	25.320666950265412	24.810998390885384
48	25.752314279637112	25.29096113119954	24.24837931066372	24.708345278499625
49	24.826924521867795	25.430564626597384	24.78218903344758	24.960321818087237
50	25.001271558699774	25.410019882554213	24.446525176862256	25.142183381883754
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	25.0
4	45.0
5	53.0
6	61.0
7	60.5
8	60.0
9	76.5
10	93.0
11	152.5
12	212.0
13	414.5
14	617.0
15	829.0
16	1041.0
17	1111.5
18	1182.0
19	1263.0
20	1344.0
21	1657.5
22	1971.0
23	2342.5
24	2714.0
25	3699.5
26	4685.0
27	6365.0
28	8045.0
29	9598.5
30	11152.0
31	12752.0
32	14352.0
33	16679.5
34	19007.0
35	22436.5
36	25866.0
37	30905.5
38	35945.0
39	38535.5
40	41126.0
41	44371.0
42	47616.0
43	47759.0
44	47902.0
45	52447.5
46	56993.0
47	59849.5
48	62706.0
49	65590.0
50	68474.0
51	65209.0
52	61944.0
53	61645.5
54	61347.0
55	61650.0
56	61953.0
57	60239.0
58	58525.0
59	53218.5
60	47912.0
61	41942.0
62	35972.0
63	31032.5
64	26093.0
65	22058.0
66	18023.0
67	15630.5
68	13238.0
69	12090.5
70	10943.0
71	8168.0
72	5393.0
73	4711.5
74	4030.0
75	3068.0
76	2106.0
77	1886.0
78	1666.0
79	1399.0
80	1132.0
81	917.0
82	702.0
83	570.5
84	439.0
85	332.5
86	226.0
87	155.0
88	84.0
89	66.5
90	49.0
91	42.0
92	35.0
93	28.0
94	21.0
95	20.5
96	20.0
97	15.5
98	11.0
99	11.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008322814220915232
2	8.091624937000921E-4
3	5.7797320978578E-4
4	0.0
5	0.0
6	0.0
7	2.31189283914312E-4
8	0.0
9	0.002080703555228808
10	0.0012715410615287159
11	0.0
12	3.46783925871468E-4
13	5.7797320978578E-4
14	1.15594641957156E-4
15	0.00520175888807202
16	8.091624937000921E-4
17	0.0
18	1.15594641957156E-4
19	0.0
20	6.93567851742936E-4
21	0.0
22	1.15594641957156E-4
23	0.0012715410615287159
24	5.7797320978578E-4
25	0.0016183249874001842
26	0.005432948171986332
27	0.006704489233515048
28	0.003814623184586148
29	0.0025430821230574318
30	0.0034678392587146802
31	0.006820083875472204
32	0.003583433900671836
33	0.004508191036329084
34	0.003005460690886056
35	0.003814623184586148
36	0.00924757135657248
37	0.003583433900671836
38	0.007976030295043764
39	0.004854974962200552
40	0.002427487481100276
41	0.003699028542628992
42	0.0025430821230574318
43	0.0016183249874001842
44	0.001387135703485872
45	0.00115594641957156
46	9.24757135657248E-4
47	0.00231189283914312
48	0.0032366499748003683
49	8.091624937000921E-4
50	0.001387135703485872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	865092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.87488392277966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24131729607275	37.77018399711955
2	14.334081113555794	14.584894255837183
3	4.954211208492536	7.561347604829752
4	2.1613673699567344	4.398372562441296
5	1.1558993441300247	2.9403122479516077
6	0.7102245310330875	2.1679554345251364
7	0.46173975985031424	1.6443669679441826
8	0.3167186987101089	1.289042162644049
9	0.25034030279300035	1.1462430461208664
>10	1.2305445826441215	11.148154371663738
>50	0.08686441533625411	3.0676180578510333
>100	0.08686223032126947	8.57632159539751
>500	0.008914807838493645	3.184779832200251
>1k	9.143392654865275E-4	0.5204078634738962
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1189	0.13744202928705848	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1178	0.13617048822552977	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1088	0.12576697044938573	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1020	0.11790653479629913	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	983	0.11362953304388435	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	977	0.11293596519214141	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	931	0.10761861166211223	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	928	0.10727182773624076	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	924	0.10680944916841215	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	889	0.10276363669991168	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	877	0.10137650099642581	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	868	0.10033614921881141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
2	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
3	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
4	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
5	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
6	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
7	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
8	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
9	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
10	0.0	0.0	0.0	1.15594641957156E-4	1.15594641957156E-4
11	0.0	0.0	0.0	2.31189283914312E-4	1.15594641957156E-4
12	0.0	0.0	0.0	2.31189283914312E-4	2.31189283914312E-4
13	0.0	0.0	0.0	3.46783925871468E-4	3.46783925871468E-4
14	0.0	0.0	0.0	3.46783925871468E-4	3.46783925871468E-4
15	0.0	0.0	0.0	3.46783925871468E-4	4.62378567828624E-4
16	0.0	0.0	0.0	4.62378567828624E-4	4.62378567828624E-4
17	0.0	0.0	0.0	4.62378567828624E-4	4.62378567828624E-4
18	0.0	0.0	0.0	4.62378567828624E-4	4.62378567828624E-4
19	0.0	0.0	0.0	4.62378567828624E-4	4.62378567828624E-4
20	0.0	0.0	0.0	6.93567851742936E-4	4.62378567828624E-4
21	0.0	0.0	0.0	6.93567851742936E-4	4.62378567828624E-4
22	0.0	0.0	0.0	0.00115594641957156	4.62378567828624E-4
23	0.0	0.0	0.0	0.001271541061528716	4.62378567828624E-4
24	0.0	0.0	0.0	0.001387135703485872	4.62378567828624E-4
25	0.0	0.0	0.0	0.001502730345443028	4.62378567828624E-4
26	0.0	0.0	0.0	0.001849514271314496	4.62378567828624E-4
27	0.0	0.0	0.0	0.002196298197185964	4.62378567828624E-4
28	0.0	0.0	0.0	0.004277001752414772	4.62378567828624E-4
29	0.0	0.0	0.0	0.007166867801343672	4.62378567828624E-4
30	0.0	0.0	0.0	0.015374087380301748	4.62378567828624E-4
31	0.0	0.0	0.0	0.026702362292103037	4.62378567828624E-4
32	0.0	0.0	0.0	0.04138288182066185	5.7797320978578E-4
33	0.0	0.0	0.0	0.05363591386812038	5.7797320978578E-4
34	0.0	0.0	0.0	0.06808524411276488	5.7797320978578E-4
35	0.0	0.0	0.0	0.08634919754199553	5.7797320978578E-4
36	0.0	0.0	0.0	0.11594142588302747	5.7797320978578E-4
37	0.0	0.0	0.0	0.1584802541232609	5.7797320978578E-4
38	0.0	0.0	0.0	0.2233288482612254	5.7797320978578E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1135	0.0	25.780888	1
ATTTCGT	105	1.1277734E-10	25.141075	42
GTCCTAA	190	0.0	24.316875	1
GGTATCA	510	0.0	23.72655	1
CTAGGAC	110	5.2496034E-9	21.999712	3
GATCGTA	60	2.872119E-4	21.99971	12
GTCTTAG	145	1.2732926E-11	21.242327	1
GATTTCG	125	9.968062E-10	21.1185	41
ATAATAC	75	5.4708835E-5	20.533064	3
TATATGC	75	5.4708835E-5	20.533064	4
TCTATAC	75	5.4708835E-5	20.533064	3
GTATAAA	110	1.2639975E-7	20.000893	1
GGCGTTA	330	0.0	19.99858	42
GTAACGG	55	0.0044844393	19.99858	16
ATAGGGG	80	8.985713E-5	19.249746	3
CGCAATA	275	0.0	19.199747	36
ATACACT	300	0.0	19.066416	4
CGTGATT	70	8.1246486E-4	18.855804	28
CGAATGC	270	0.0	18.73941	43
CATTAGG	60	0.0074085337	18.334152	1
>>END_MODULE