FastQCFastQC Report
Thu 2 Feb 2017
SRR4064051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150675
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32290.2806178981901927No Hit
TATCAACGCAGAGTACTTTTTTTTT22740.1976231342472896No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20550.17859082712321028No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13360.11610576400808222No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13030.11323788211267301No Hit
GTACATGGGGTGGTATCAACGCAAA12200.1060247246181589No Hit
GTCCTAAAGTGTGTATTTCTCATTT11940.10376518130662438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT755.8547812E-815.1970431
GCGCCAT400.005280241414.2490876
CGAGCCG2100.013.1210615
CCAACGA801.9927247E-613.0656419
GTGTAGG1051.9967047E-812.6642031
GTCCTAA3650.012.4907211
CGAGATT550.003073084412.0880324
AGAACCG951.0408767E-611.9981885
AGGCCCG2300.011.98009910
CGGTCCA2400.011.876821510
CCAACGT658.0044783E-411.69511819
AACAATC658.016171E-411.6930847
TCTCGTC658.016171E-411.69308412
AAGCTCG658.016171E-411.6930847
CTATTAG658.0337375E-411.6900341
CGACCAT1552.5465852E-1111.64694810
AACAGCG907.450217E-611.6118827
GGTCCAA2550.011.55079211
CCGAGCC2400.011.48042914
CTCGTCT752.071642E-411.40075713
Produced by FastQC (version 0.11.5)