##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064051_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150675
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238858930627675	32.0	32.0	32.0	32.0	32.0
2	30.883139027092792	32.0	32.0	32.0	32.0	32.0
3	30.903991135637778	32.0	32.0	32.0	32.0	32.0
4	30.92722445521107	32.0	32.0	32.0	32.0	32.0
5	30.861304017207292	32.0	32.0	32.0	32.0	32.0
6	34.51302757077367	36.0	36.0	36.0	32.0	36.0
7	34.44059790992244	36.0	36.0	36.0	32.0	36.0
8	34.39554174723532	36.0	36.0	36.0	32.0	36.0
9	34.508909118560844	36.0	36.0	36.0	32.0	36.0
10	34.2528246464032	36.0	36.0	36.0	32.0	36.0
11	34.49876376909206	36.0	36.0	36.0	32.0	36.0
12	34.33381450018467	36.0	36.0	36.0	32.0	36.0
13	34.39746670432572	36.0	36.0	36.0	32.0	36.0
14	34.318658178895	36.0	36.0	36.0	32.0	36.0
15	34.2641805896539	36.0	36.0	36.0	32.0	36.0
16	34.25729072066396	36.0	36.0	36.0	32.0	36.0
17	34.191495426597434	36.0	36.0	36.0	32.0	36.0
18	34.18927412171117	36.0	36.0	36.0	32.0	36.0
19	34.1929980228996	36.0	36.0	36.0	32.0	36.0
20	34.158871097399356	36.0	36.0	36.0	32.0	36.0
21	34.14057835618224	36.0	36.0	36.0	32.0	36.0
22	34.12563582245204	36.0	36.0	36.0	32.0	36.0
23	34.079293458187585	36.0	36.0	36.0	32.0	36.0
24	34.06243465791818	36.0	36.0	36.0	32.0	36.0
25	33.65996132704717	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	7.0
5	8.0
6	48.0
7	17.0
8	57.0
9	74.0
10	114.0
11	31.0
12	77.0
13	66.0
14	98.0
15	203.0
16	372.0
17	448.0
18	612.0
19	801.0
20	1189.0
21	1635.0
22	2529.0
23	3886.0
24	5829.0
25	8374.0
26	12245.0
27	16324.0
28	22301.0
29	30655.0
30	39675.0
31	54410.0
32	77374.0
33	109313.0
34	235291.0
35	526603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.97524003602714	17.635127851134193	11.586498169084125	25.803133943754542
2	15.857807968448123	20.14934647879	39.20831765328568	24.784527899476192
3	19.095972627669035	24.564007679131205	28.625795310654578	27.714224382545183
4	12.295753728599824	15.812079090378948	36.708490022448515	35.18367715857272
5	13.928772571460357	37.32834565188672	34.07178019899737	14.671101577655557
6	33.114650938222226	36.26816232908919	17.45773893181125	13.159447800877336
7	29.21455164362711	30.71488239440966	21.397967512536802	18.672598449426424
8	27.173777914171232	34.10538596691029	19.943108860812316	18.77772725810616
9	27.507442856944113	14.129863941792687	18.85199148592813	39.51070171533507
10	15.641646825983441	27.179880938509243	32.28051624617172	24.897955989335596
11	36.69294077239784	21.154988239692884	23.40888044484829	18.743190543060983
12	24.716655434050047	23.603017588103572	30.164315064025914	21.516011913820464
13	29.706619220671705	20.05784654664291	25.61622833906842	24.619305893616968
14	23.02218210247754	20.454634395781863	25.95793862200852	30.565244879732077
15	24.36185291790221	28.68144386375297	22.802507891510214	24.154195326834614
16	24.448429338950064	26.117099081831608	25.501044440463417	23.933427138754915
17	23.04854696514073	26.27872111366004	26.3600298450873	24.31270207611193
18	23.630628146800387	25.548735887505337	27.96598302804278	22.854652937651494
19	25.4331660564103	24.802355212691012	25.918993903234504	23.845484827664183
20	25.350318028147207	25.053555539899858	25.884612273546825	23.71151415840611
21	26.23907948007671	24.502928808429402	24.91622346786573	24.341768243628156
22	25.337744692583403	25.344006874484133	25.582491635203553	23.735756797728914
23	23.843226007344487	25.181046284876306	26.30681235028781	24.668915357491393
24	24.432960191757257	25.35800109063679	26.343314022510256	23.8657246950957
25	24.2488471567258	25.344374420534265	26.392587709151254	24.01419071358868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	135.0
1	135.0
2	365.0
3	595.0
4	595.0
5	595.0
6	1647.5
7	2700.0
8	2700.0
9	2700.0
10	2747.5
11	2795.0
12	2795.0
13	2795.0
14	3057.0
15	3319.0
16	3319.0
17	3319.0
18	5829.5
19	8340.0
20	8340.0
21	8340.0
22	13923.0
23	19506.0
24	19506.0
25	19506.0
26	30030.0
27	40554.0
28	40554.0
29	40554.0
30	52544.5
31	64535.0
32	64535.0
33	64535.0
34	74769.5
35	85004.0
36	85004.0
37	85004.0
38	94035.0
39	103066.0
40	103066.0
41	103066.0
42	114621.5
43	126177.0
44	126177.0
45	126177.0
46	137645.0
47	149113.0
48	149113.0
49	149113.0
50	149111.0
51	149109.0
52	149109.0
53	149109.0
54	135632.5
55	122156.0
56	122156.0
57	122156.0
58	110690.5
59	99225.0
60	99225.0
61	99225.0
62	86612.5
63	74000.0
64	74000.0
65	74000.0
66	61283.0
67	48566.0
68	48566.0
69	48566.0
70	36485.5
71	24405.0
72	24405.0
73	24405.0
74	18765.5
75	13126.0
76	13126.0
77	13126.0
78	10533.0
79	7940.0
80	7940.0
81	7940.0
82	5481.0
83	3022.0
84	3022.0
85	3022.0
86	2354.0
87	1686.0
88	1686.0
89	1686.0
90	1191.0
91	696.0
92	696.0
93	696.0
94	491.0
95	286.0
96	286.0
97	286.0
98	452.5
99	619.0
100	619.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03745627566428401
2	0.005388141742890043
3	0.002085732287570339
4	0.0036500315032480936
5	0.00929888978208443
6	0.012862015773350423
7	0.022769244139309534
8	0.035457448888695765
9	0.05031829143763443
10	0.05918265365980837
11	0.05388141742890043
12	0.0651791339865731
13	0.06396245681882373
14	0.0681339213939644
15	0.06126838594737871
16	0.06752558281008972
17	0.06404936233080583
18	0.07786733873595933
19	0.07691137810415626
20	0.08116974819127903
21	0.07734590566406674
22	0.07960544897560128
23	0.08421144111065244
24	0.07717209464010255
25	0.07899711039172659
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1150675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.7951136155794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3289679470241	41.06125502870079
2	14.294186608753115	15.379147853203348
3	4.358838324326993	7.034526086673369
4	1.8054176337313894	3.8849058692060248
5	0.9058759756091621	2.4365850514759355
6	0.5232307106766033	1.6888353316804938
7	0.3576749868044227	1.3468816586816348
8	0.22526083545650055	0.9694345789218227
9	0.16172401770052364	0.7829965715910878
>10	0.863349584273346	8.456463733382504
>50	0.08053403076911077	3.023711169218645
>100	0.08471511059622124	9.186105778230552
>500	0.007952182216599981	3.000631085776985
>1k	0.0022720520618857093	1.7485202032569525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3229	0.2806178981901927	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2274	0.1976231342472896	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2055	0.17859082712321028	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1336	0.11610576400808222	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1303	0.11323788211267301	No Hit
GTACATGGGGTGGTATCAACGCAAA	1220	0.1060247246181589	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1194	0.10376518130662438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.690551198209746E-5	0.0
5	0.0	0.0	0.0	8.690551198209746E-5	0.0
6	0.0	0.0	0.0	8.690551198209746E-5	0.0
7	0.0	0.0	0.0	8.690551198209746E-5	0.0
8	0.0	0.0	0.0	8.690551198209746E-5	0.0
9	0.0	0.0	0.0	2.6071653594629237E-4	0.0
10	0.0	0.0	0.0	6.083385838746823E-4	0.0
11	0.0	0.0	0.0	6.083385838746823E-4	0.0
12	0.0	0.0	0.0	6.952440958567797E-4	0.0
13	0.0	0.0	0.0	7.821496078388772E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	75	5.8547812E-8	15.197043	1
GCGCCAT	40	0.0052802414	14.249087	6
CGAGCCG	210	0.0	13.12106	15
CCAACGA	80	1.9927247E-6	13.06564	19
GTGTAGG	105	1.9967047E-8	12.664203	1
GTCCTAA	365	0.0	12.490721	1
CGAGATT	55	0.0030730844	12.088032	4
AGAACCG	95	1.0408767E-6	11.998188	5
AGGCCCG	230	0.0	11.980099	10
CGGTCCA	240	0.0	11.8768215	10
CCAACGT	65	8.0044783E-4	11.695118	19
AACAATC	65	8.016171E-4	11.693084	7
TCTCGTC	65	8.016171E-4	11.693084	12
AAGCTCG	65	8.016171E-4	11.693084	7
CTATTAG	65	8.0337375E-4	11.690034	1
CGACCAT	155	2.5465852E-11	11.646948	10
AACAGCG	90	7.450217E-6	11.611882	7
GGTCCAA	255	0.0	11.550792	11
CCGAGCC	240	0.0	11.480429	14
CTCGTCT	75	2.071642E-4	11.400757	13
>>END_MODULE