##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150675
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297689616963957	32.0	32.0	32.0	32.0	32.0
2	31.390276142264323	32.0	32.0	32.0	32.0	32.0
3	31.465613661546485	32.0	32.0	32.0	32.0	32.0
4	31.553257870380428	32.0	32.0	32.0	32.0	32.0
5	31.496731049166794	32.0	32.0	32.0	32.0	32.0
6	35.079161361809376	36.0	36.0	36.0	36.0	36.0
7	35.08942750993982	36.0	36.0	36.0	36.0	36.0
8	35.03681752015122	36.0	36.0	36.0	36.0	36.0
9	35.153369978490886	36.0	36.0	36.0	36.0	36.0
10	35.006584830642886	36.0	36.0	36.0	36.0	36.0
11	35.14760119060551	36.0	36.0	36.0	36.0	36.0
12	35.05377017837356	36.0	36.0	36.0	36.0	36.0
13	35.102747517761316	36.0	36.0	36.0	36.0	36.0
14	35.04707410867534	36.0	36.0	36.0	36.0	36.0
15	35.01722206530949	36.0	36.0	36.0	36.0	36.0
16	35.027737632259324	36.0	36.0	36.0	36.0	36.0
17	34.99545571077846	36.0	36.0	36.0	36.0	36.0
18	34.99310839289982	36.0	36.0	36.0	36.0	36.0
19	34.98686683902927	36.0	36.0	36.0	36.0	36.0
20	34.97180350663741	36.0	36.0	36.0	36.0	36.0
21	34.96064657700915	36.0	36.0	36.0	36.0	36.0
22	34.947007625958676	36.0	36.0	36.0	36.0	36.0
23	34.89957807373933	36.0	36.0	36.0	32.0	36.0
24	34.877129510939234	36.0	36.0	36.0	32.0	36.0
25	34.840059095748146	36.0	36.0	36.0	32.0	36.0
26	34.79002150911422	36.0	36.0	36.0	32.0	36.0
27	34.77360679601104	36.0	36.0	36.0	32.0	36.0
28	34.746935494383735	36.0	36.0	36.0	32.0	36.0
29	34.712701675103744	36.0	36.0	36.0	32.0	36.0
30	34.693638951050474	36.0	36.0	36.0	32.0	36.0
31	34.68638494796532	36.0	36.0	36.0	32.0	36.0
32	34.643840354574486	36.0	36.0	36.0	32.0	36.0
33	34.61290633758446	36.0	36.0	36.0	32.0	36.0
34	34.6034901253612	36.0	36.0	36.0	32.0	36.0
35	34.555467008495015	36.0	36.0	36.0	32.0	36.0
36	34.51687574684424	36.0	36.0	36.0	32.0	36.0
37	34.49648423751277	36.0	36.0	36.0	32.0	36.0
38	34.45124035891976	36.0	36.0	36.0	32.0	36.0
39	34.43161796336933	36.0	36.0	36.0	32.0	36.0
40	34.41352423577465	36.0	36.0	36.0	32.0	36.0
41	34.39613878810263	36.0	36.0	36.0	32.0	36.0
42	34.328873052773375	36.0	36.0	36.0	32.0	36.0
43	34.31260347187521	36.0	36.0	36.0	32.0	36.0
44	34.231975145023576	36.0	36.0	36.0	32.0	36.0
45	34.20714276402981	36.0	36.0	36.0	32.0	36.0
46	34.14376170508614	36.0	36.0	36.0	32.0	36.0
47	34.11805939991744	36.0	36.0	36.0	32.0	36.0
48	34.06784452603907	36.0	36.0	36.0	32.0	36.0
49	34.035672105503295	36.0	36.0	36.0	32.0	36.0
50	33.432692984552546	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	1.0
20	8.0
21	34.0
22	127.0
23	367.0
24	946.0
25	2090.0
26	4504.0
27	8390.0
28	13987.0
29	21539.0
30	30951.0
31	44160.0
32	62690.0
33	99890.0
34	202562.0
35	658424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29055197426687	18.550312494839556	12.156748906420159	26.002386624473417
2	15.187678755639139	21.01240762934065	38.598790436470104	25.201123178550105
3	18.338643264730063	25.15647351030787	29.043586628358685	27.46129659660338
4	11.943685228235601	16.504399591544093	36.848806135529145	34.70310904469116
5	13.511460664392638	37.803202468116545	33.791730940534904	14.893605926955916
6	33.01204253865787	36.70753847103125	17.1446051912319	13.13581379907897
7	28.9795989310622	30.905164360049536	21.446889868989942	18.66834683989832
8	27.070719360375435	34.4427401307928	19.791687487778912	18.69485302105286
9	27.469886847548363	13.997010411416058	18.752020579493507	39.781082161542066
10	15.44577739461737	27.578511190060695	32.19010936294174	24.78560205238019
11	36.84440597423771	21.153862866459136	23.401502076174484	18.60022908312867
12	24.676841572852084	23.779106853896064	30.057948645750233	21.486102927501623
13	29.765267192157612	20.062919864079188	25.67825701547794	24.49355592828526
14	23.03556266637003	20.436909530335726	25.990702832168655	30.536824971125593
15	24.37418467798637	28.757477496895152	22.716815905177995	24.151521919940482
16	24.464571883462476	26.216510757229717	25.299912746335174	24.019004612972637
17	23.08045277771743	26.554152997153846	26.22191322484629	24.143481000282442
18	23.807827078437644	25.520826091188454	27.880403798823643	22.79094303155026
19	25.417016461656385	24.82049650900255	26.059074942164333	23.703412087176734
20	25.494081279531684	25.0038455822733	25.86238811962759	23.639685018567427
21	26.272666043843827	24.45738370956178	25.043735198904994	24.2262150476894
22	25.33089302443611	25.25719708622946	25.78158540125179	23.63032448808264
23	23.870672820799836	25.065114633911058	26.427372740759026	24.636839804530084
24	24.579267958838003	25.263302432576413	26.36639601346343	23.791033595122162
25	24.417594107023984	25.22495824128455	26.39559278256441	23.961854869127055
26	23.417783676062495	26.263126652917524	26.969530260720987	23.349559410298994
27	24.305586939819854	25.563265462206196	26.13087643793042	24.000271160043525
28	23.535843035281605	25.808515002242256	26.432435157148966	24.223206805327177
29	23.836158835302797	25.264654530348473	26.082191733201466	24.816994901147265
30	23.508272431861183	25.800670763556283	26.561124393701185	24.129932410881352
31	24.643007997663798	25.242702414248043	25.707595955452266	24.406693632635893
32	24.032489914255965	25.90902059212573	25.642558798019177	24.415930695599123
33	23.636776062950517	25.5058585907747	26.425277546501096	24.432087799773686
34	24.326356087238025	25.65639271886638	26.57952713478093	23.43772405911467
35	24.72204719961413	25.670819409983096	26.327494579127357	23.279638811275415
36	23.631585031240686	26.104939843540187	26.113196527325712	24.150278597893415
37	24.49334497907677	25.954277420728555	25.410403820499116	24.141973779695558
38	24.12474121612343	25.950960129465816	25.837973046496792	24.086325607913963
39	24.788052551669114	25.2451029384542	25.585435571017495	24.381408938859188
40	25.38150341764836	25.583737816615898	25.932846360084994	23.101912405650744
41	24.12591666854969	25.55747527015541	26.80921996047396	23.50738810082094
42	24.94839895537759	26.593461984695672	25.75289726286333	22.70524179706341
43	23.961010236724803	25.6733303727162	26.36745314215047	23.998206248408525
44	23.57557464996085	26.339560790052985	25.55270597580339	24.532158584182774
45	23.762598237354833	26.45332188480147	25.702098887852763	24.081980989990935
46	23.835471673189016	25.6378301477675	25.595245854766702	24.931452324276783
47	24.50421543978148	25.65339099344632	25.876308281138243	23.96608528563396
48	24.608093803719264	25.998168004741633	25.024898797036133	24.368839394502974
49	24.223973858708405	26.213249671930267	25.318292820704457	24.24448364865687
50	24.135602529685215	26.807988827252604	24.835289751854813	24.22111889120737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	21.0
2	20.0
3	69.0
4	118.0
5	146.0
6	174.0
7	192.5
8	211.0
9	309.0
10	407.0
11	600.5
12	794.0
13	1396.5
14	1999.0
15	2795.5
16	3592.0
17	3991.5
18	4391.0
19	4403.5
20	4416.0
21	4816.0
22	5216.0
23	5965.0
24	6714.0
25	7720.0
26	8726.0
27	11586.5
28	14447.0
29	17305.5
30	20164.0
31	23049.5
32	25935.0
33	30686.0
34	35437.0
35	41567.0
36	47697.0
37	51787.5
38	55878.0
39	57201.0
40	58524.0
41	60634.5
42	62745.0
43	62734.0
44	62723.0
45	67250.0
46	71777.0
47	75325.5
48	78874.0
49	81584.5
50	84295.0
51	80544.0
52	76793.0
53	75526.0
54	74259.0
55	74452.0
56	74645.0
57	72451.0
58	70257.0
59	63331.0
60	56405.0
61	49259.0
62	42113.0
63	36390.0
64	30667.0
65	25954.0
66	21241.0
67	18554.5
68	15868.0
69	14203.0
70	12538.0
71	9838.0
72	7138.0
73	6012.5
74	4887.0
75	3864.5
76	2842.0
77	2538.5
78	2235.0
79	1837.5
80	1440.0
81	1152.0
82	864.0
83	743.0
84	622.0
85	444.0
86	266.0
87	191.5
88	117.0
89	90.5
90	64.0
91	48.0
92	32.0
93	30.5
94	29.0
95	23.5
96	18.0
97	20.5
98	23.0
99	19.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008342929150281356
2	0.0010428661437851695
3	8.690551198209746E-5
4	0.0
5	0.0
6	3.4762204792838984E-4
7	0.0
8	0.0
9	0.001303582679731462
10	9.559606318030722E-4
11	8.690551198209746E-5
12	8.690551198209746E-5
13	4.3452755991048737E-4
14	1.7381102396419492E-4
15	0.004866708670997458
16	6.083385838746823E-4
17	0.0
18	2.6071653594629237E-4
19	8.690551198209746E-5
20	3.4762204792838984E-4
21	0.0
22	8.690551198209746E-5
23	0.0010428661437851695
24	1.7381102396419492E-4
25	0.0014773937036956569
26	0.005040519694961653
27	0.00547504725487214
28	0.0040845590631585806
29	0.002085732287570339
30	0.003997653551176483
31	0.0077345905664066746
32	0.0035631259912659963
33	0.004953614182979555
34	0.002607165359462924
35	0.002607165359462924
36	0.007995307102352966
37	0.003476220479283899
38	0.008777456710191844
39	0.0031285984313555084
40	0.002607165359462924
41	0.0035631259912659963
42	0.0017381102396419495
43	0.0012166771677493645
44	0.0015642992156777542
45	0.0015642992156777542
46	0.0013904881917135594
47	0.002085732287570339
48	0.0014773937036956569
49	4.3452755991048737E-4
50	0.0015642992156777542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1150675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.30830871911704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53685514362655	45.210428868240356
2	13.652569531105103	15.921164780577746
3	4.254703683422975	7.442537276441737
4	1.707750769786762	3.983042364001452
5	0.8386394281125235	2.4449823339204393
6	0.49053016517797887	1.716119058434224
7	0.3082368647636228	1.2580939188475033
8	0.201126685837237	0.9381885511560384
9	0.15562758689142012	0.8166943243508523
>10	0.7184366829501104	7.470599488644644
>50	0.06470819931440398	2.6885498792706195
>100	0.06451390551332235	7.465828191126836
>500	0.005851256819607434	2.3664553910816424
>1k	4.5009667843134103E-4	0.2773155739059113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.7381102396419492E-4
6	0.0	0.0	0.0	0.0	1.7381102396419492E-4
7	0.0	0.0	0.0	0.0	1.7381102396419492E-4
8	0.0	0.0	0.0	8.690551198209746E-5	1.7381102396419492E-4
9	0.0	0.0	0.0	8.690551198209746E-5	1.7381102396419492E-4
10	0.0	0.0	0.0	3.4762204792838984E-4	1.7381102396419492E-4
11	0.0	0.0	0.0	3.4762204792838984E-4	1.7381102396419492E-4
12	0.0	0.0	0.0	3.4762204792838984E-4	2.6071653594629237E-4
13	0.0	0.0	0.0	3.4762204792838984E-4	3.4762204792838984E-4
14	0.0	0.0	0.0	3.4762204792838984E-4	3.4762204792838984E-4
15	0.0	0.0	0.0	3.4762204792838984E-4	4.345275599104873E-4
16	0.0	0.0	0.0	3.4762204792838984E-4	4.345275599104873E-4
17	0.0	0.0	0.0	3.4762204792838984E-4	4.345275599104873E-4
18	0.0	0.0	0.0	3.4762204792838984E-4	4.345275599104873E-4
19	0.0	0.0	0.0	3.4762204792838984E-4	4.345275599104873E-4
20	0.0	0.0	0.0	6.083385838746823E-4	4.345275599104873E-4
21	0.0	0.0	0.0	6.083385838746823E-4	4.345275599104873E-4
22	0.0	0.0	0.0	8.690551198209746E-4	4.345275599104873E-4
23	0.0	0.0	0.0	0.0010428661437851695	4.345275599104873E-4
24	0.0	0.0	0.0	0.0015642992156777544	4.345275599104873E-4
25	0.0	0.0	0.0	0.0016512047276598517	4.345275599104873E-4
26	0.0	0.0	0.0	0.0019988267755882416	5.214330718925847E-4
27	0.0	0.0	0.0	0.0027809763834271187	5.214330718925847E-4
28	0.0	0.0	0.0	0.004345275599104873	5.214330718925847E-4
29	0.0	0.0	0.0	0.008342929150281356	6.083385838746823E-4
30	0.0	0.0	0.0	0.01442631498902818	6.083385838746823E-4
31	0.0	0.0	0.0	0.0308514567536446	6.083385838746823E-4
32	0.0	0.0	0.0	0.04684207095835053	6.083385838746823E-4
33	0.0	0.0	0.0	0.061876724531253396	6.083385838746823E-4
34	0.0	0.0	0.0	0.07899711039172659	6.083385838746823E-4
35	0.0	0.0	0.0	0.10002824429139417	6.083385838746823E-4
36	0.0	0.0	0.0	0.12844634670954005	6.083385838746823E-4
37	0.0	0.0	0.0	0.17224672474851718	6.083385838746823E-4
38	0.0	0.0	0.0	0.23029960675255828	6.083385838746823E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGT	30	0.005744512	29.33374	6
TATTAGG	100	1.7462298E-9	24.19823	2
GTAGGAC	1030	0.0	21.359518	3
TGTAGGA	1110	0.0	20.412924	2
AATCACG	550	0.0	20.401169	34
GGCGTTA	260	0.0	20.30709	42
GGCGAGA	755	0.0	19.813122	19
GATATAC	535	0.0	19.73859	1
GTATCAA	2590	0.0	19.367064	1
GCGAGAA	720	0.0	19.24943	20
TAGGACC	880	0.0	18.75026	4
CTGTAGG	1140	0.0	18.719557	1
AGGACCT	1055	0.0	18.350965	5
GTGTAGG	205	0.0	18.244156	1
CGGCGTT	285	0.0	17.754631	41
ATACGAA	285	0.0	17.754631	40
TGGCGAG	1165	0.0	17.749775	18
GTCCTAC	825	0.0	17.600243	1
TAAGGCG	75	0.0012906994	17.600243	5
CACCGGT	75	0.0012906994	17.600243	7
>>END_MODULE