FastQCFastQC Report
Thu 2 Feb 2017
SRR4064050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658103
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24280.36893920860412427No Hit
TATCAACGCAGAGTACTTTTTTTTT18780.2853656646451999No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14570.22139391554209598No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9670.14693748546959975No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9440.14344259181313562No Hit
GGTATCAACGCAGAGTACTTTTTTT9040.13736451588885024No Hit
GTACATGGGGTGGTATCAACGCAAA9030.1372125639907431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA250.006024979519.00191
TCCAACG453.52625E-516.89314718
TCTATAC456.765458E-414.7747633
CGGTTCT1203.6379788E-1214.25034212
CGCATCG602.5650785E-514.2492613
TAGAACC759.670548E-713.9304914
TAATCCC551.9621046E-413.8143145
GTATTAG903.621426E-813.7235941
GAACCGC903.62852E-813.7215086
ATTAGCT707.2626153E-613.5686613
GTATAGA500.001496815813.301331
AACCGCG500.001497673713.3003197
GACCGCG1501.8189894E-1212.666977
ATAGGAC1900.012.4974513
CGCCAGT701.0864963E-412.21736518
TCGCCAG701.0872679E-412.21643617
GAATAGG1950.012.1807051
CGCGGTT1501.4551915E-1112.03362210
GTCTAAC802.8575476E-511.8761881
ATCGCCA802.8575476E-511.87618816
Produced by FastQC (version 0.11.5)