##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064050_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658103
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14469467545354	32.0	32.0	32.0	32.0	32.0
2	30.700540796805363	32.0	32.0	32.0	32.0	32.0
3	30.72077319203833	32.0	32.0	32.0	32.0	32.0
4	30.75132767970971	32.0	32.0	32.0	32.0	32.0
5	30.638506434403126	32.0	32.0	32.0	32.0	32.0
6	34.287176931270636	36.0	36.0	36.0	32.0	36.0
7	34.20591153664396	36.0	36.0	36.0	32.0	36.0
8	34.13885668352826	36.0	36.0	36.0	32.0	36.0
9	34.306338065621944	36.0	36.0	36.0	32.0	36.0
10	33.97453286187724	36.0	36.0	36.0	32.0	36.0
11	34.317272524209734	36.0	36.0	36.0	32.0	36.0
12	34.0949106750767	36.0	36.0	36.0	32.0	36.0
13	34.193917973326364	36.0	36.0	36.0	32.0	36.0
14	34.082500763558286	36.0	36.0	36.0	32.0	36.0
15	34.015138967608415	36.0	36.0	36.0	32.0	36.0
16	34.02013514601818	36.0	36.0	36.0	32.0	36.0
17	33.951004629974335	36.0	36.0	36.0	32.0	36.0
18	33.93867373344294	36.0	36.0	36.0	32.0	36.0
19	33.96107296274291	36.0	36.0	36.0	32.0	36.0
20	33.92370647147939	36.0	36.0	36.0	32.0	36.0
21	33.905382592086646	36.0	36.0	36.0	32.0	36.0
22	33.87570942542429	36.0	36.0	36.0	32.0	36.0
23	33.84159774381822	36.0	36.0	36.0	32.0	36.0
24	33.833618749648615	36.0	36.0	36.0	32.0	36.0
25	33.42957865258174	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	8.0
5	12.0
6	37.0
7	15.0
8	37.0
9	28.0
10	67.0
11	12.0
12	32.0
13	44.0
14	75.0
15	129.0
16	194.0
17	269.0
18	375.0
19	463.0
20	762.0
21	1129.0
22	1638.0
23	2699.0
24	4001.0
25	6034.0
26	8239.0
27	11124.0
28	14754.0
29	19987.0
30	26391.0
31	35202.0
32	49457.0
33	68852.0
34	139740.0
35	266294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.904265927304124	17.673257694599492	11.961894859727847	25.460581518368535
2	16.1743945562105	20.452048487169318	38.050909400353454	25.322647556266727
3	18.439811149589644	24.82528905752583	29.161037978261234	27.573861814623296
4	12.287049348478517	15.988907039471185	36.793830490445615	34.93021312160468
5	14.257035741368593	37.14479365016648	33.85179770619406	14.74637290227086
6	32.819509793194	36.37676137676137	17.491124727966834	13.312604102077785
7	29.109379797126206	31.177178674237705	21.56894334793433	18.14449818070176
8	27.837872348187737	33.00818421164639	19.995682919562455	19.158260520603417
9	27.617214638736737	14.259739825384735	18.962767696024105	39.16027783985442
10	15.756216598374676	27.372646206943735	32.233414168750905	24.637723025930686
11	36.44196123147093	21.48962371721779	23.050247054351956	19.01816799695933
12	24.318003846943352	24.189518980027827	29.659172983205735	21.833304189823085
13	29.465734282747096	20.06343616621735	25.47101961625073	24.99980993478482
14	23.3238297257639	20.038166487998843	26.33959050855705	30.298413277680208
15	24.640717652425117	27.69195681921849	23.18017333130607	24.487152197050328
16	24.74967345405776	26.128845373083493	24.783430473465007	24.338050699393744
17	23.292649364195775	26.164754109577586	26.035662208003696	24.506934318222942
18	23.84302346800978	25.55582928817353	27.15321118775624	23.44793605606044
19	24.925258743130343	25.257526589031055	26.297365275653057	23.519849392185545
20	25.408325273281385	24.61615903547031	25.92112706900013	24.054388622248176
21	26.552836354733287	24.466268341679502	25.25658201880512	23.724313284782088
22	25.2417233146281	24.898641969676234	25.78782487035601	24.071809845339658
23	24.04716968219472	24.66842371806473	26.232128490846957	25.05227810889359
24	24.051327829288432	25.441583267054657	26.267950369310363	24.239138534346548
25	24.365919162487113	25.138921416253275	26.12133230732733	24.37382711393229
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	255.0
1	255.0
2	379.0
3	503.0
4	503.0
5	503.0
6	1202.5
7	1902.0
8	1902.0
9	1902.0
10	1945.0
11	1988.0
12	1988.0
13	1988.0
14	2127.0
15	2266.0
16	2266.0
17	2266.0
18	3872.5
19	5479.0
20	5479.0
21	5479.0
22	8679.0
23	11879.0
24	11879.0
25	11879.0
26	17316.5
27	22754.0
28	22754.0
29	22754.0
30	27856.0
31	32958.0
32	32958.0
33	32958.0
34	38989.0
35	45020.0
36	45020.0
37	45020.0
38	50741.0
39	56462.0
40	56462.0
41	56462.0
42	64069.0
43	71676.0
44	71676.0
45	71676.0
46	78705.5
47	85735.0
48	85735.0
49	85735.0
50	86866.0
51	87997.0
52	87997.0
53	87997.0
54	80640.5
55	73284.0
56	73284.0
57	73284.0
58	66266.0
59	59248.0
60	59248.0
61	59248.0
62	51235.5
63	43223.0
64	43223.0
65	43223.0
66	35205.0
67	27187.0
68	27187.0
69	27187.0
70	20361.0
71	13535.0
72	13535.0
73	13535.0
74	10322.0
75	7109.0
76	7109.0
77	7109.0
78	5692.0
79	4275.0
80	4275.0
81	4275.0
82	2932.0
83	1589.0
84	1589.0
85	1589.0
86	1230.0
87	871.0
88	871.0
89	871.0
90	616.5
91	362.0
92	362.0
93	362.0
94	257.5
95	153.0
96	153.0
97	153.0
98	273.0
99	393.0
100	393.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03631650364760531
2	0.004862460739428327
3	0.0024312303697141633
4	0.004254653146999785
5	0.010636632867499465
6	0.014435430320177845
7	0.02385644800282023
8	0.03813992642489093
9	0.04771289600564046
10	0.05895733646556846
11	0.053639020031818724
12	0.06655493137092522
13	0.06594712377849668
14	0.06959396933306793
15	0.06123661493717549
16	0.07020177692549646
17	0.06564321998228241
18	0.08007865030246025
19	0.07612790095167474
20	0.08190207307974587
21	0.07840717942328176
22	0.08099036169110306
23	0.08539696673620999
24	0.07992669840435312
25	0.0815981692835316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	658103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.06906396655692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72577696468491	50.912991911357196
2	12.685549951210001	16.001315226476333
3	3.3482075407874254	6.335049466896911
4	1.2265456102208476	3.094283341956729
5	0.5864031580392736	1.8491949142284965
6	0.32826213124421505	1.2421911211943821
7	0.2088489693532813	0.9220336305244109
8	0.13437653873391375	0.6780002013610975
9	0.09182570440487903	0.5212225102396917
>10	0.5331035280084796	6.404737284821292
>50	0.06748508306341026	3.0159657302747758
>100	0.060712242899760085	7.161570717807693
>500	0.002176933012294121	0.9822846703886301
>1k	7.256443374313735E-4	0.8791592724723832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2428	0.36893920860412427	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1878	0.2853656646451999	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1457	0.22139391554209598	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	967	0.14693748546959975	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	944	0.14344259181313562	No Hit
GGTATCAACGCAGAGTACTTTTTTT	904	0.13736451588885024	No Hit
GTACATGGGGTGGTATCAACGCAAA	903	0.1372125639907431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.558556943214056E-4
2	0.0	0.0	0.0	0.0	4.558556943214056E-4
3	0.0	0.0	0.0	0.0	4.558556943214056E-4
4	0.0	0.0	0.0	0.0	4.558556943214056E-4
5	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
6	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
7	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
8	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
9	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
10	0.0	0.0	0.0	3.039037962142704E-4	4.558556943214056E-4
11	0.0	0.0	0.0	3.039037962142704E-4	4.558556943214056E-4
12	0.0	0.0	0.0	3.039037962142704E-4	6.078075924285408E-4
13	0.0	0.0	0.0	3.039037962142704E-4	6.078075924285408E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	25	0.0060249795	19.0019	1
TCCAACG	45	3.52625E-5	16.893147	18
TCTATAC	45	6.765458E-4	14.774763	3
CGGTTCT	120	3.6379788E-12	14.250342	12
CGCATCG	60	2.5650785E-5	14.24926	13
TAGAACC	75	9.670548E-7	13.930491	4
TAATCCC	55	1.9621046E-4	13.814314	5
GTATTAG	90	3.621426E-8	13.723594	1
GAACCGC	90	3.62852E-8	13.721508	6
ATTAGCT	70	7.2626153E-6	13.568661	3
GTATAGA	50	0.0014968158	13.30133	1
AACCGCG	50	0.0014976737	13.300319	7
GACCGCG	150	1.8189894E-12	12.66697	7
ATAGGAC	190	0.0	12.497451	3
CGCCAGT	70	1.0864963E-4	12.217365	18
TCGCCAG	70	1.0872679E-4	12.216436	17
GAATAGG	195	0.0	12.180705	1
CGCGGTT	150	1.4551915E-11	12.033622	10
GTCTAAC	80	2.8575476E-5	11.876188	1
ATCGCCA	80	2.8575476E-5	11.876188	16
>>END_MODULE