FastQCFastQC Report
Thu 2 Feb 2017
SRR4064050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658103
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.10864560714660167No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.10074410844503064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT904.0735176E-722.00136842
GTATAGA1057.739982E-820.9504991
GATTTCG956.852424E-720.84340141
TGCACCG654.931866E-420.3058685
CGTCTTA550.004479865520.00124432
GTATTAG2150.019.4401151
CATCGTT1901.8189894E-1218.52887528
AGTGTGC2300.018.1722818
GGTCGGA1852.1827873E-1117.83759138
ACGCCGC2100.017.8079246
CTATAAC1002.4821424E-517.598423
AACGCCG2150.017.3937875
CCAGTCG2055.456968E-1217.17049420
GTATTAT902.2149607E-417.1095751
GTTATAT902.2149607E-417.1095751
ATCGCCA2201.8189894E-1216.99847416
ATACCGT1702.4629117E-916.8220186
TAACGCC2109.094947E-1216.7603994
AATAACG2109.094947E-1216.7603992
GTATCAA18250.016.6341231
Produced by FastQC (version 0.11.5)