##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658103
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264145582074537	32.0	32.0	32.0	32.0	32.0
2	31.38592287225556	32.0	32.0	32.0	32.0	32.0
3	31.461496148779144	32.0	32.0	32.0	32.0	32.0
4	31.543062408164072	32.0	32.0	32.0	32.0	32.0
5	31.48434059714057	32.0	32.0	32.0	32.0	32.0
6	35.05314973491991	36.0	36.0	36.0	36.0	36.0
7	35.07478768521037	36.0	36.0	36.0	36.0	36.0
8	35.02823874074423	36.0	36.0	36.0	36.0	36.0
9	35.141442904834044	36.0	36.0	36.0	36.0	36.0
10	34.983520816650284	36.0	36.0	36.0	36.0	36.0
11	35.14016954792791	36.0	36.0	36.0	36.0	36.0
12	35.04218640547148	36.0	36.0	36.0	36.0	36.0
13	35.07426041212394	36.0	36.0	36.0	36.0	36.0
14	35.029493863422594	36.0	36.0	36.0	36.0	36.0
15	34.99815682347596	36.0	36.0	36.0	36.0	36.0
16	35.01785434802759	36.0	36.0	36.0	36.0	36.0
17	34.96860673785107	36.0	36.0	36.0	36.0	36.0
18	34.96929811898745	36.0	36.0	36.0	36.0	36.0
19	34.96441286546331	36.0	36.0	36.0	36.0	36.0
20	34.96354370060614	36.0	36.0	36.0	36.0	36.0
21	34.95734406316336	36.0	36.0	36.0	36.0	36.0
22	34.948381940213004	36.0	36.0	36.0	36.0	36.0
23	34.896807946476464	36.0	36.0	36.0	32.0	36.0
24	34.870717805571466	36.0	36.0	36.0	32.0	36.0
25	34.842744980648924	36.0	36.0	36.0	32.0	36.0
26	34.79160404982199	36.0	36.0	36.0	32.0	36.0
27	34.78689202146169	36.0	36.0	36.0	32.0	36.0
28	34.746911957550715	36.0	36.0	36.0	32.0	36.0
29	34.72731320173286	36.0	36.0	36.0	32.0	36.0
30	34.706384866806566	36.0	36.0	36.0	32.0	36.0
31	34.68234151796907	36.0	36.0	36.0	32.0	36.0
32	34.64900175200538	36.0	36.0	36.0	32.0	36.0
33	34.60423368378506	36.0	36.0	36.0	32.0	36.0
34	34.60135571483491	36.0	36.0	36.0	32.0	36.0
35	34.546026989696145	36.0	36.0	36.0	32.0	36.0
36	34.52571102091922	36.0	36.0	36.0	32.0	36.0
37	34.5093199696704	36.0	36.0	36.0	32.0	36.0
38	34.460877704553845	36.0	36.0	36.0	32.0	36.0
39	34.45921231175059	36.0	36.0	36.0	32.0	36.0
40	34.44700601577565	36.0	36.0	36.0	32.0	36.0
41	34.40989632321992	36.0	36.0	36.0	32.0	36.0
42	34.33335663262438	36.0	36.0	36.0	32.0	36.0
43	34.319032127189814	36.0	36.0	36.0	32.0	36.0
44	34.242071529836515	36.0	36.0	36.0	32.0	36.0
45	34.21658919652395	36.0	36.0	36.0	32.0	36.0
46	34.15988834574527	36.0	36.0	36.0	32.0	36.0
47	34.122901734227014	36.0	36.0	36.0	32.0	36.0
48	34.0596851860575	36.0	36.0	36.0	32.0	36.0
49	34.02028709791628	36.0	36.0	36.0	32.0	36.0
50	33.446185475525866	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	6.0
21	21.0
22	64.0
23	236.0
24	536.0
25	1246.0
26	2684.0
27	4904.0
28	8232.0
29	12375.0
30	17689.0
31	25088.0
32	35668.0
33	56679.0
34	115354.0
35	377317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.970008449180305	18.691380012521808	12.447952441448395	25.89065909684949
2	15.398267740464973	21.24236438231272	37.66220939067011	25.697158486552198
3	17.68740664426889	25.409473621830088	29.520544718819757	27.382575015081272
4	11.959678044318292	16.771842705473155	36.69380021060533	34.574679039603225
5	13.68721917389831	37.77098721628681	33.594133441117876	14.947660168696997
6	32.72552389374883	36.8210958500291	17.27683136782956	13.176548888392512
7	28.830745339255405	31.36089639463731	21.69158931048787	18.11676895561941
8	27.6891307287765	33.28126448291529	19.945965905033102	19.08363888327511
9	27.592772406163906	14.069069685103383	18.935584700549622	39.40257320818309
10	15.602961891404407	27.623147488272938	32.26610828560705	24.50778233471561
11	36.561602059252124	21.468068068372276	22.98758704944363	18.98274282293197
12	24.273859866920528	24.32096495533374	29.582755282987616	21.822419894758116
13	29.438231271843186	20.119282783771464	25.530770399635315	24.911715544750038
14	23.304933437268748	20.04737874581561	26.347171634749074	30.300516182166565
15	24.724195989520968	27.851190440009972	23.181820944693317	24.242792625775746
16	24.72591509788041	26.128591594881623	24.830458639201698	24.31503466803627
17	23.196672861239044	26.322171453404707	26.04835413301565	24.432801552340592
18	23.94169301765833	25.492666041637857	27.265336884955698	23.30030405574811
19	25.000037987974526	25.23951417939137	26.388726384775634	23.371721447858466
20	25.535940045221366	24.588965743599715	25.927372298266516	23.9477219129124
21	26.523811622192877	24.490543273621302	25.32703847270108	23.658606631484737
22	25.121941583523526	24.807704580748883	25.954335346192536	24.116018489535058
23	23.99722229397794	24.626612794162565	26.393525574497378	24.982639337362123
24	24.30360127640176	25.24358000303905	26.290837258775262	24.161981461783924
25	24.25336959990275	24.961327478004527	26.42465316294124	24.360649759151485
26	24.01176175605753	25.81279964745124	26.593421622484104	23.58201697400713
27	24.60345440675442	25.290931802363925	25.896045637314646	24.20956815356701
28	23.979594937939922	25.338564777106455	26.485703691997227	24.196136592956393
29	23.952644487194682	25.268126050394628	26.342909589324194	24.436319873086497
30	23.84389550079627	25.675458004595242	26.46670880997824	24.013937684630253
31	24.414213574410336	25.384053114747125	25.69147796433993	24.510255346502603
32	24.201727153509392	25.488962537818523	25.691066546923842	24.618243761748246
33	23.98186813520791	25.225508610052454	26.116298011755624	24.676325242984007
34	24.536874527985074	25.292098413118104	26.129986643022235	24.041040415874594
35	24.875167532313604	25.181360377582124	26.20752429028601	23.73594779981826
36	24.02887318592812	25.91748347390016	25.83177570093458	24.22186763923714
37	24.72761587532177	25.679174809065188	25.718987718781054	23.874221596831987
38	24.239932891005633	25.5895592021081	26.023883264390594	24.146624642495677
39	24.733424610489173	25.378493744576936	25.59534030625968	24.29274133867421
40	24.87626978277882	25.539557959838017	26.012597156902224	23.571575100480942
41	23.972276968944552	25.861558075677614	26.351315415579542	23.814849539798292
42	24.83041678569909	26.154867767640607	25.634265534491036	23.38044991216927
43	24.299181872333182	25.41833311046547	25.864929918187237	24.417555099014113
44	24.076803617720266	25.822298942096417	25.441654231766282	24.659243208417035
45	24.09894680218997	26.14547228270862	25.476415875615988	24.27916503948542
46	23.894495154940106	25.75213533649499	25.53560059140577	24.817768917159125
47	24.4113249291888	25.64170749200715	25.785457264074108	24.161510314729945
48	24.844778069868863	26.107371332188606	24.903280706286374	24.144569891656158
49	24.190391675378667	26.16320415258564	25.101808854635188	24.5445953174005
50	24.240610004041976	26.312046607323573	25.057514579984012	24.389828808650435
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	71.0
2	30.0
3	86.5
4	143.0
5	154.0
6	165.0
7	170.5
8	176.0
9	240.5
10	305.0
11	454.5
12	604.0
13	1101.0
14	1598.0
15	2172.0
16	2746.0
17	2996.5
18	3247.0
19	3271.5
20	3296.0
21	3543.0
22	3790.0
23	4374.0
24	4958.0
25	5322.0
26	5686.0
27	6692.0
28	7698.0
29	8909.0
30	10120.0
31	11555.0
32	12990.0
33	14865.0
34	16740.0
35	18887.5
36	21035.0
37	23876.5
38	26718.0
39	28526.5
40	30335.0
41	32760.5
42	35186.0
43	36158.0
44	37130.0
45	40282.0
46	43434.0
47	45875.5
48	48317.0
49	49503.5
50	50690.0
51	48993.5
52	47297.0
53	46218.5
54	45140.0
55	44539.5
56	43939.0
57	42339.5
58	40740.0
59	36525.0
60	32310.0
61	28408.0
62	24506.0
63	21200.5
64	17895.0
65	15164.0
66	12433.0
67	10652.0
68	8871.0
69	7904.5
70	6938.0
71	5335.0
72	3732.0
73	3176.5
74	2621.0
75	2090.0
76	1559.0
77	1354.5
78	1150.0
79	952.0
80	754.0
81	598.0
82	442.0
83	363.0
84	284.0
85	213.0
86	142.0
87	91.5
88	41.0
89	29.5
90	18.0
91	15.5
92	13.0
93	12.0
94	11.0
95	10.0
96	9.0
97	8.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0077495468034638965
2	4.558556943214056E-4
3	3.039037962142704E-4
4	0.0
5	0.0
6	3.039037962142704E-4
7	0.0
8	0.0
9	0.0021273265734998927
10	0.001519518981071352
11	0.0
12	0.0
13	4.558556943214056E-4
14	3.039037962142704E-4
15	0.004102701248892651
16	6.078075924285408E-4
17	0.0
18	0.0
19	0.0
20	0.0010636632867499463
21	0.0
22	1.519518981071352E-4
23	9.117113886428112E-4
24	4.558556943214056E-4
25	0.0019753746753927576
26	0.005774172128071138
27	0.006837835414821084
28	0.004710508841321192
29	0.0025831822678212984
30	0.004710508841321192
31	0.00850930629399957
32	0.003950749350785515
33	0.005318316433749732
34	0.003039037962142704
35	0.003190989860249839
36	0.008053450599678167
37	0.00379879745267838
38	0.01048468096939233
39	0.005166364535642597
40	0.0027351341659284335
41	0.0033429417583569748
42	0.002887086064035569
43	0.002279278471607028
44	0.0013675670829642167
45	0.0018234227772856225
46	0.001519518981071352
47	0.002279278471607028
48	0.0019753746753927576
49	0.0010636632867499463
50	0.0013675670829642167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	658103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.46424992433654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51679704954606	54.17952772132447
2	12.317534386806352	16.373513678726134
3	3.265576879533473	6.511323536053433
4	1.1543701203397834	3.068973767337992
5	0.5338322908538606	1.7740381398496048
6	0.2738116369262942	1.0919211041316548
7	0.17827439220737473	0.8294211631146103
8	0.11439463609242669	0.6082522946600462
9	0.08455451243442892	0.5057867022005075
>10	0.45194038991840074	5.728430209918587
>50	0.06249910645536669	2.994510974578964
>100	0.04503594743421323	5.7976482817538555
>500	0.0013786514519994134	0.5366524263502234
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	715	0.10864560714660167	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.10074410844503064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.519518981071352E-4
9	0.0	0.0	0.0	0.0	1.519518981071352E-4
10	0.0	0.0	0.0	0.0	1.519518981071352E-4
11	0.0	0.0	0.0	0.0	1.519518981071352E-4
12	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
13	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
14	0.0	0.0	0.0	1.519518981071352E-4	4.558556943214056E-4
15	0.0	0.0	0.0	1.519518981071352E-4	9.117113886428112E-4
16	0.0	0.0	0.0	1.519518981071352E-4	9.117113886428112E-4
17	0.0	0.0	0.0	1.519518981071352E-4	9.117113886428112E-4
18	0.0	0.0	0.0	1.519518981071352E-4	9.117113886428112E-4
19	0.0	0.0	0.0	1.519518981071352E-4	9.117113886428112E-4
20	0.0	0.0	0.0	1.519518981071352E-4	0.0010636632867499463
21	0.0	0.0	0.0	4.558556943214056E-4	0.0010636632867499463
22	0.0	0.0	0.0	0.0010636632867499463	0.0010636632867499463
23	0.0	0.0	0.0	0.0019753746753927576	0.0010636632867499463
24	0.0	0.0	0.0	0.003039037962142704	0.0010636632867499463
25	0.0	0.0	0.0	0.0031909898602498392	0.0010636632867499463
26	0.0	0.0	0.0	0.004102701248892651	0.0013675670829642167
27	0.0	0.0	0.0	0.005470268331856867	0.0013675670829642167
28	0.0	0.0	0.0	0.009117113886428113	0.0013675670829642167
29	0.0	0.0	0.0	0.014283478422070709	0.0013675670829642167
30	0.0	0.0	0.0	0.023552544206605956	0.001519518981071352
31	0.0	0.0	0.0	0.04680118461699764	0.0016714708791784872
32	0.0	0.0	0.0	0.06959396933306793	0.0016714708791784872
33	0.0	0.0	0.0	0.08949966798510263	0.0016714708791784872
34	0.0	0.0	0.0	0.11669905774627984	0.0016714708791784872
35	0.0	0.0	0.0	0.15119213861659953	0.0016714708791784872
36	0.0	0.0	0.0	0.1937386700865974	0.0016714708791784872
37	0.0	0.0	0.0	0.24950501669191602	0.0016714708791784872
38	0.0	0.0	0.0	0.334901983428126	0.0016714708791784872
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTCGT	90	4.0735176E-7	22.001368	42
GTATAGA	105	7.739982E-8	20.950499	1
GATTTCG	95	6.852424E-7	20.843401	41
TGCACCG	65	4.931866E-4	20.305868	5
CGTCTTA	55	0.0044798655	20.001244	32
GTATTAG	215	0.0	19.440115	1
CATCGTT	190	1.8189894E-12	18.528875	28
AGTGTGC	230	0.0	18.172281	8
GGTCGGA	185	2.1827873E-11	17.837591	38
ACGCCGC	210	0.0	17.807924	6
CTATAAC	100	2.4821424E-5	17.59842	3
AACGCCG	215	0.0	17.393787	5
CCAGTCG	205	5.456968E-12	17.170494	20
GTATTAT	90	2.2149607E-4	17.109575	1
GTTATAT	90	2.2149607E-4	17.109575	1
ATCGCCA	220	1.8189894E-12	16.998474	16
ATACCGT	170	2.4629117E-9	16.822018	6
TAACGCC	210	9.094947E-12	16.760399	4
AATAACG	210	9.094947E-12	16.760399	2
GTATCAA	1825	0.0	16.634123	1
>>END_MODULE