FastQCFastQC Report
Thu 2 Feb 2017
SRR4064049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270606
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23940.18841403235936238No Hit
TATCAACGCAGAGTACTTTTTTTTT17670.13906750007476748No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13150.1034939233719973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT456.7766535E-414.7733071
AACCGCG759.671621E-713.9329597
TAGGAGT655.468474E-513.1493484
CTTATAC957.423114E-812.9945323
CGGTGAG1102.73576E-912.95725915
GCATCGC1850.012.8400214
CGCCTCG905.3963777E-712.66782211
AATCGAA751.478487E-512.6663267
GTCCTAG1650.012.6628341
TAATAGA701.0940844E-412.210114
CAAGACG2650.012.1845114
ATCGCCA1950.012.18155916
CGCATCG1950.012.18107913
ACGTTCG550.003068532812.0905837
GGCTAGT550.00307487512.0872511
AAGACGG2600.012.0564185
CGCAAGA2700.011.957462
GTACTAA658.042038E-411.688771
TCGCCAG2150.011.49032617
GTTTAGA752.0790796E-411.3965511
Produced by FastQC (version 0.11.5)