##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064049_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1270606
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193174752834473	32.0	32.0	32.0	32.0	32.0
2	30.897738559396068	32.0	32.0	32.0	32.0	32.0
3	30.914650961824517	32.0	32.0	32.0	32.0	32.0
4	30.93939112517964	32.0	32.0	32.0	32.0	32.0
5	30.890938654468812	32.0	32.0	32.0	32.0	32.0
6	34.47447517168973	36.0	36.0	36.0	32.0	36.0
7	34.422862791455415	36.0	36.0	36.0	32.0	36.0
8	34.39770314322457	36.0	36.0	36.0	32.0	36.0
9	34.49245871654943	36.0	36.0	36.0	32.0	36.0
10	34.29230068172195	36.0	36.0	36.0	32.0	36.0
11	34.46274061353402	36.0	36.0	36.0	32.0	36.0
12	34.33121518393585	36.0	36.0	36.0	32.0	36.0
13	34.379518119700364	36.0	36.0	36.0	32.0	36.0
14	34.31413671901439	36.0	36.0	36.0	32.0	36.0
15	34.261600370217046	36.0	36.0	36.0	32.0	36.0
16	34.26736690996265	36.0	36.0	36.0	32.0	36.0
17	34.20406010990032	36.0	36.0	36.0	32.0	36.0
18	34.19100807016495	36.0	36.0	36.0	32.0	36.0
19	34.19580341978552	36.0	36.0	36.0	32.0	36.0
20	34.17329054010449	36.0	36.0	36.0	32.0	36.0
21	34.15437987857762	36.0	36.0	36.0	32.0	36.0
22	34.13770909314138	36.0	36.0	36.0	32.0	36.0
23	34.09069766709743	36.0	36.0	36.0	32.0	36.0
24	34.08007989888289	36.0	36.0	36.0	32.0	36.0
25	33.664970100881	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	3.0
4	9.0
5	14.0
6	50.0
7	22.0
8	68.0
9	79.0
10	127.0
11	48.0
12	73.0
13	58.0
14	126.0
15	249.0
16	343.0
17	500.0
18	639.0
19	880.0
20	1242.0
21	1832.0
22	2692.0
23	4080.0
24	6232.0
25	9138.0
26	13189.0
27	17547.0
28	24538.0
29	33395.0
30	44511.0
31	61088.0
32	86662.0
33	123123.0
34	265885.0
35	572162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13734756109563	17.29211659878482	11.35006011342423	26.22047572669532
2	16.46508523561297	19.650667394967638	37.808265324032284	26.075982045387107
3	18.70988560387538	23.64863429050398	28.736526875415656	28.90495323020498
4	12.460834106616232	15.407966742145373	36.104873689255975	36.026325461982424
5	14.41490935880564	36.57359599025269	34.04955828553078	14.96193636541089
6	34.24999665470494	35.66619780060625	16.82691285939292	13.256892685295893
7	30.083372496601257	30.434228145125296	20.98749688071459	18.49490247755886
8	27.8490908890502	33.3503655496787	19.50364604902444	19.296897512246662
9	27.21791592911992	14.059950739226569	18.50657020290147	40.21556312875204
10	15.407942772725697	27.007899934167217	32.293815813097886	25.2903414800092
11	37.00484446068364	21.024581385679546	22.78266703624841	19.1879071173884
12	24.26814257487496	23.596234733036336	29.538193298719424	22.597429393369282
13	29.38380750324067	19.58476860099008	25.37167328976735	25.659750606001904
14	23.259927576066396	19.67314722787077	25.551015714954012	31.51590948110882
15	24.81275067312107	27.66202746664714	22.352108272675654	25.173113587556138
16	25.399583071144882	25.8958181894073	24.102409823580974	24.602188915866844
17	23.76222419275833	26.08421049315729	25.56547473732456	24.588090576759818
18	24.43208400478245	25.367938363727728	26.406892651629356	23.793084979860467
19	25.260381388637732	25.16901949788765	25.49122768328298	24.07937143019164
20	25.463291147216633	24.719205552562514	25.194705154015555	24.622798146205298
21	26.2701625606452	24.385671980341503	24.74206099174595	24.602104467267345
22	25.40738086142868	24.681975528399892	25.165421558164354	24.745222052007072
23	24.283055382489923	24.469887499025276	25.697213822741062	25.54984329574374
24	24.59784255843333	25.124128501353127	25.526679751616975	24.75134918859656
25	24.712376154571384	24.764832505924968	25.544431553519264	24.978359785984388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	237.5
3	390.0
4	390.0
5	390.0
6	1181.5
7	1973.0
8	1973.0
9	1973.0
10	2159.5
11	2346.0
12	2346.0
13	2346.0
14	2883.0
15	3420.0
16	3420.0
17	3420.0
18	5926.0
19	8432.0
20	8432.0
21	8432.0
22	13506.0
23	18580.0
24	18580.0
25	18580.0
26	27682.0
27	36784.0
28	36784.0
29	36784.0
30	46649.5
31	56515.0
32	56515.0
33	56515.0
34	69417.5
35	82320.0
36	82320.0
37	82320.0
38	94979.5
39	107639.0
40	107639.0
41	107639.0
42	122644.0
43	137649.0
44	137649.0
45	137649.0
46	151928.5
47	166208.0
48	166208.0
49	166208.0
50	170183.0
51	174158.0
52	174158.0
53	174158.0
54	161749.5
55	149341.0
56	149341.0
57	149341.0
58	135678.5
59	122016.0
60	122016.0
61	122016.0
62	106160.0
63	90304.0
64	90304.0
65	90304.0
66	73152.5
67	56001.0
68	56001.0
69	56001.0
70	41883.5
71	27766.0
72	27766.0
73	27766.0
74	20851.0
75	13936.0
76	13936.0
77	13936.0
78	11221.5
79	8507.0
80	8507.0
81	8507.0
82	5770.0
83	3033.0
84	3033.0
85	3033.0
86	2341.0
87	1649.0
88	1649.0
89	1649.0
90	1196.0
91	743.0
92	743.0
93	743.0
94	491.5
95	240.0
96	240.0
97	240.0
98	405.5
99	571.0
100	571.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03990221988562938
2	0.005430479629405182
3	0.001652754669818968
4	0.0038564275629109257
5	0.008027665539120703
6	0.012985929548577609
7	0.022272836740893715
8	0.03651800794266673
9	0.04958264009456905
10	0.056823279600442624
11	0.05524922753394836
12	0.06209635402319838
13	0.06359170348636792
14	0.06768423885925298
15	0.060994517576652404
16	0.0665036998093823
17	0.06201765141987367
18	0.07586930960502312
19	0.07319342109198289
20	0.0782303877047645
21	0.07287861067868402
22	0.0764202278282961
23	0.08004054758123288
24	0.07508228357177599
25	0.07704984865489381
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1270606.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.47795959385474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45472179420975	46.46350010622767
2	12.476230830154908	14.59169044738807
3	3.7071566206745223	6.503608652156874
4	1.486431352774466	3.476938903463363
5	0.7725972371745731	2.258995500890925
6	0.4637669866612998	1.6272088264165954
7	0.30574309743658334	1.251546274859756
8	0.206878636117954	0.9678272418989995
9	0.16538180749906783	0.8704071590840237
>10	0.8265243594704507	8.65746640259744
>50	0.06368113890479522	2.6082956573569662
>100	0.06427011352987748	7.562794282503273
>500	0.004860753349059054	1.898611630486833
>1k	0.0017552720427157695	1.2611089146692047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2394	0.18841403235936238	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1767	0.13906750007476748	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1315	0.1034939233719973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.870260332471277E-5
2	0.0	0.0	0.0	0.0	7.870260332471277E-5
3	0.0	0.0	0.0	0.0	7.870260332471277E-5
4	0.0	0.0	0.0	0.0	7.870260332471277E-5
5	0.0	0.0	0.0	0.0	7.870260332471277E-5
6	0.0	0.0	0.0	0.0	7.870260332471277E-5
7	0.0	0.0	0.0	0.0	7.870260332471277E-5
8	0.0	0.0	0.0	0.0	7.870260332471277E-5
9	0.0	0.0	0.0	0.0	7.870260332471277E-5
10	0.0	0.0	0.0	0.0	7.870260332471277E-5
11	0.0	0.0	0.0	0.0	7.870260332471277E-5
12	0.0	0.0	0.0	0.0	1.5740520664942555E-4
13	0.0	0.0	0.0	0.0	1.5740520664942555E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAT	45	6.7766535E-4	14.773307	1
AACCGCG	75	9.671621E-7	13.932959	7
TAGGAGT	65	5.468474E-5	13.149348	4
CTTATAC	95	7.423114E-8	12.994532	3
CGGTGAG	110	2.73576E-9	12.957259	15
GCATCGC	185	0.0	12.84002	14
CGCCTCG	90	5.3963777E-7	12.667822	11
AATCGAA	75	1.478487E-5	12.666326	7
GTCCTAG	165	0.0	12.662834	1
TAATAGA	70	1.0940844E-4	12.21011	4
CAAGACG	265	0.0	12.184511	4
ATCGCCA	195	0.0	12.181559	16
CGCATCG	195	0.0	12.181079	13
ACGTTCG	55	0.0030685328	12.090583	7
GGCTAGT	55	0.003074875	12.087251	1
AAGACGG	260	0.0	12.056418	5
CGCAAGA	270	0.0	11.95746	2
GTACTAA	65	8.042038E-4	11.68877	1
TCGCCAG	215	0.0	11.490326	17
GTTTAGA	75	2.0790796E-4	11.396551	1
>>END_MODULE