FastQCFastQC Report
Thu 2 Feb 2017
SRR4064049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270606
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.023.875541
GTACGTA500.002581957521.99787511
GTATAAA1850.020.2158551
TAGGACG2550.018.9793054
ACGTAGT708.128914E-418.8553213
AACGCAG24550.018.459236
CGCAATA2400.018.33444836
CGTAGTC600.007416436418.33156214
GGATAGT1254.855865E-717.5989916
TCAACGC26850.016.6321954
CGTTTAG1206.398559E-616.50230229
ATCAACG27100.016.4781133
CAACGCA27350.016.4085665
TATCAAC27900.016.3998492
TAAGACT2051.05501385E-1016.096644
TAGCGTA2600.016.0760027
CGTATAT2650.015.77268210
ATAGCGT2650.015.7726826
CGTAAAC1401.5865808E-615.714004540
AAGACGG5500.015.5991085
Produced by FastQC (version 0.11.5)