##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1270606
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262506237181313	32.0	32.0	32.0	32.0	32.0
2	31.25694825933452	32.0	32.0	32.0	32.0	32.0
3	31.374315090594568	32.0	32.0	32.0	32.0	32.0
4	31.482598854404905	32.0	32.0	32.0	32.0	32.0
5	31.406076313192287	32.0	32.0	32.0	32.0	32.0
6	34.96876608484455	36.0	36.0	36.0	36.0	36.0
7	34.9981961363318	36.0	36.0	36.0	36.0	36.0
8	34.92686560586051	36.0	36.0	36.0	36.0	36.0
9	35.07148636162587	36.0	36.0	36.0	36.0	36.0
10	34.88615589726477	36.0	36.0	36.0	32.0	36.0
11	35.06869241920784	36.0	36.0	36.0	36.0	36.0
12	34.964990720963065	36.0	36.0	36.0	36.0	36.0
13	35.0179646562349	36.0	36.0	36.0	36.0	36.0
14	34.95812785395315	36.0	36.0	36.0	32.0	36.0
15	34.91673107163039	36.0	36.0	36.0	32.0	36.0
16	34.94005616217773	36.0	36.0	36.0	36.0	36.0
17	34.89694208905042	36.0	36.0	36.0	32.0	36.0
18	34.89810767460566	36.0	36.0	36.0	32.0	36.0
19	34.89933858332166	36.0	36.0	36.0	32.0	36.0
20	34.89468647243913	36.0	36.0	36.0	32.0	36.0
21	34.88730259419521	36.0	36.0	36.0	32.0	36.0
22	34.8679126338141	36.0	36.0	36.0	32.0	36.0
23	34.81567063275319	36.0	36.0	36.0	32.0	36.0
24	34.789955344142875	36.0	36.0	36.0	32.0	36.0
25	34.756951407438656	36.0	36.0	36.0	32.0	36.0
26	34.699542580469476	36.0	36.0	36.0	32.0	36.0
27	34.68807561116507	36.0	36.0	36.0	32.0	36.0
28	34.67144732513462	36.0	36.0	36.0	32.0	36.0
29	34.635651807090476	36.0	36.0	36.0	32.0	36.0
30	34.618112931939564	36.0	36.0	36.0	32.0	36.0
31	34.610083692348375	36.0	36.0	36.0	32.0	36.0
32	34.574553402077434	36.0	36.0	36.0	32.0	36.0
33	34.55285981649701	36.0	36.0	36.0	32.0	36.0
34	34.53841080555262	36.0	36.0	36.0	32.0	36.0
35	34.50649611287842	36.0	36.0	36.0	32.0	36.0
36	34.47822771181625	36.0	36.0	36.0	32.0	36.0
37	34.47594061416363	36.0	36.0	36.0	32.0	36.0
38	34.43058036873744	36.0	36.0	36.0	32.0	36.0
39	34.42741101490155	36.0	36.0	36.0	32.0	36.0
40	34.406831858184205	36.0	36.0	36.0	32.0	36.0
41	34.394204812506786	36.0	36.0	36.0	32.0	36.0
42	34.325252674707976	36.0	36.0	36.0	32.0	36.0
43	34.329534883354874	36.0	36.0	36.0	32.0	36.0
44	34.25808472492653	36.0	36.0	36.0	32.0	36.0
45	34.23466125612503	36.0	36.0	36.0	32.0	36.0
46	34.2130432250438	36.0	36.0	36.0	32.0	36.0
47	34.18854231760278	36.0	36.0	36.0	32.0	36.0
48	34.15161977827903	36.0	36.0	36.0	32.0	36.0
49	34.13482228165143	36.0	36.0	36.0	32.0	36.0
50	33.49692587631414	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	6.0
20	12.0
21	31.0
22	119.0
23	364.0
24	979.0
25	2315.0
26	4770.0
27	9013.0
28	15259.0
29	23759.0
30	35106.0
31	50291.0
32	74650.0
33	120443.0
34	244136.0
35	689350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29062832792469	18.274788095071308	11.94664458610357	26.487938990900428
2	15.817699011964406	20.459514466371058	37.073607628509954	26.649178893154584
3	17.987601162597603	24.34458284767154	29.05628272560351	28.61153326412735
4	12.102571528861032	16.035655427410227	36.26836328492074	35.593409758808
5	14.071317151028722	37.15290184368719	33.65024248272084	15.12553852256325
6	34.40033244031972	35.995636720803645	16.519702440728974	13.084328398147651
7	29.886762694336404	30.668279545350803	20.969442927233146	18.475514833079647
8	27.933206674610382	33.58853964171427	19.19879175763376	19.279461926041588
9	27.308086734713953	13.888856095647132	18.358365070632708	40.444692099006204
10	15.3482602922901	27.230406103635563	32.25847580685148	25.16285779722286
11	37.35532493943835	20.954095919584827	22.631169693831133	19.059409447145693
12	24.358553602417746	23.594744236013554	29.381908618335363	22.66479354323334
13	29.54074489159067	19.577507022267415	25.223811408931677	25.657936677210238
14	23.190527347208615	19.652527732851674	25.51304299920117	31.64390192073855
15	24.93010955799018	27.68306888301221	22.289462913990683	25.097358645006928
16	25.521092397292616	25.882889973240992	23.93813946167165	24.657878167794742
17	23.942276362617523	26.153347300422002	25.342238270557516	24.562138066402962
18	24.609475838186682	25.299779631670077	26.269557689280653	23.821186840862584
19	25.441010037730027	25.098811118474178	25.38371454250964	24.07646430128616
20	25.6872748107584	24.725995161333483	25.013418878354916	24.573311149553202
21	26.52718466621439	24.388205313055344	24.578901720911126	24.505708299819144
22	25.514911782251932	24.741029083760033	25.05883019598522	24.685228938002812
23	24.457736502439793	24.375255784668663	25.545726428458998	25.621281284432552
24	24.78773807649929	25.192192664882736	25.301038879269637	24.71903037934834
25	24.913897861141017	24.757040856802856	25.36424195488059	24.964819327175537
26	24.572190451004374	25.465431288379293	25.728783464064943	24.233594796551394
27	24.974892328228893	25.0970462673786	25.056826768083823	24.871234636308685
28	24.449021610977223	24.904884310672948	25.70870436453904	24.93738971381078
29	24.583928208825302	25.023572023353225	25.502410721453455	24.89008904636802
30	24.58272041027869	25.239600771633725	25.609519019700898	24.568159798386684
31	25.02081834596077	24.853878036763074	25.024202802960062	25.101100814316098
32	24.73305225147513	25.163490000920845	24.83584100641682	25.267616741187204
33	24.48450971163397	24.82106005487439	25.411990825933582	25.282439407558062
34	25.069378092106405	24.895283549010134	25.47431318443475	24.561025174448716
35	25.449482595240568	24.881155888843765	25.44775109163432	24.221610424281348
36	24.548388950237975	25.4512568604502	25.123828518851138	24.87652567046069
37	25.288573996621956	25.101254724302237	24.952894712401953	24.657276566673854
38	24.691204493345538	25.02422272788451	25.327488919725365	24.95708385904458
39	25.27147585110444	24.87755351008024	24.848196260689225	25.002774378126098
40	25.479527740633557	25.066407046269674	25.2719048807113	24.182160332385465
41	24.577057572115667	25.189305843157715	25.700811372596245	24.532825212130373
42	25.505379830770064	25.55338943898805	24.991834431389155	23.949396298852726
43	24.86557404918038	24.90264350048954	25.336300988203885	24.895481462126195
44	24.68977035096266	25.280361658472316	24.904080069825774	25.125787920739246
45	24.704724022128286	25.45823164181078	25.041732548081956	24.79531178797898
46	24.638593663426185	25.179207802347253	24.941365914471366	25.2408326197552
47	24.90335092115702	25.026916925866693	25.329771694097797	24.739960458878492
48	25.454732913525387	25.465987607244244	24.51335255288722	24.565926926343145
49	24.765917123997617	25.531069253272282	24.742306175758326	24.960707446971778
50	24.859435159154657	25.672051050379824	24.65818975151662	24.8103240389489
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	15.0
2	17.0
3	63.0
4	109.0
5	139.5
6	170.0
7	164.0
8	158.0
9	215.0
10	272.0
11	396.0
12	520.0
13	1032.5
14	1545.0
15	2054.5
16	2564.0
17	2706.0
18	2848.0
19	2964.5
20	3081.0
21	3571.5
22	4062.0
23	4802.0
24	5542.0
25	6891.0
26	8240.0
27	10233.0
28	12226.0
29	14401.5
30	16577.0
31	18941.5
32	21306.0
33	24707.0
34	28108.0
35	32375.5
36	36643.0
37	43090.0
38	49537.0
39	53449.0
40	57361.0
41	62276.5
42	67192.0
43	69603.0
44	72014.0
45	78080.5
46	84147.0
47	89411.0
48	94675.0
49	97051.5
50	99428.0
51	97729.0
52	96030.0
53	94525.0
54	93020.0
55	92464.0
56	91908.0
57	88453.5
58	84999.0
59	76823.5
60	68648.0
61	60418.5
62	52189.0
63	44674.0
64	37159.0
65	31401.5
66	25644.0
67	21449.0
68	17254.0
69	15374.5
70	13495.0
71	10501.5
72	7508.0
73	6454.5
74	5401.0
75	4271.5
76	3142.0
77	2688.0
78	2234.0
79	1881.0
80	1528.0
81	1206.0
82	884.0
83	749.0
84	614.0
85	453.0
86	292.0
87	203.0
88	114.0
89	90.5
90	67.0
91	55.0
92	43.0
93	33.5
94	24.0
95	25.0
96	26.0
97	19.0
98	12.0
99	14.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008421178555744268
2	3.148104132988511E-4
3	2.3610780997413835E-4
4	0.0
5	0.0
6	1.5740520664942555E-4
7	0.0
8	0.0
9	0.0014953494631695426
10	0.0014953494631695426
11	0.0
12	7.870260332471277E-5
13	3.9351301662356387E-4
14	7.870260332471277E-5
15	0.0036203197529367875
16	4.722156199482767E-4
17	0.0
18	4.722156199482767E-4
19	0.0
20	6.296208265977022E-4
21	0.0
22	0.0
23	4.722156199482767E-4
24	4.722156199482767E-4
25	0.0017314572731436811
26	0.0050369666127816175
27	0.006138803059327597
28	0.0037777249595862135
29	0.0025184833063908087
30	0.004328643182859203
31	0.007240639505873575
32	0.0030694015296637983
33	0.005351777026080469
34	0.0025184833063908087
35	0.0026758885130402344
36	0.007791557729146565
37	0.0037777249595862135
38	0.008263773349094842
39	0.004013832769560351
40	0.0025971859097155218
41	0.003384211942962649
42	0.002124970289767245
43	0.00141664685984483
44	0.0011805390498706915
45	0.0011805390498706915
46	0.0011018364465459787
47	0.002361078099741383
48	0.001967565083117819
49	7.08323429922415E-4
50	0.00141664685984483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1270606.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.48790534975166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30227454710037	49.376186567218795
2	12.288606528166584	15.112013501684945
3	3.37941803648006	6.2338000909298845
4	1.4176167161525106	3.486651298600454
5	0.7082868789557247	2.177553828685031
6	0.45211790091061255	1.667986961887209
7	0.2870084049397864	1.235328194626455
8	0.1845114454956212	0.9076177837264495
9	0.14078880201028438	0.7791127679081964
>10	0.7246805768321258	7.88132105887383
>50	0.057010499960600765	2.430864468515423
>100	0.05252970841905095	6.402241848882853
>500	0.004119963661419992	1.6440191374392856
>1k	0.001029990915354998	0.6653024910213203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5740520664942555E-4
2	0.0	0.0	0.0	0.0	1.5740520664942555E-4
3	0.0	0.0	0.0	0.0	1.5740520664942555E-4
4	0.0	0.0	0.0	0.0	1.5740520664942555E-4
5	0.0	0.0	0.0	0.0	1.5740520664942555E-4
6	0.0	0.0	0.0	0.0	2.3610780997413832E-4
7	0.0	0.0	0.0	0.0	3.148104132988511E-4
8	0.0	0.0	0.0	0.0	3.148104132988511E-4
9	0.0	0.0	0.0	0.0	3.148104132988511E-4
10	0.0	0.0	0.0	0.0	3.148104132988511E-4
11	0.0	0.0	0.0	0.0	3.148104132988511E-4
12	0.0	0.0	0.0	0.0	7.870260332471277E-4
13	0.0	0.0	0.0	0.0	7.870260332471277E-4
14	0.0	0.0	0.0	7.870260332471277E-5	7.870260332471277E-4
15	0.0	0.0	0.0	7.870260332471277E-5	0.001023133843221266
16	0.0	0.0	0.0	7.870260332471277E-5	0.001023133843221266
17	0.0	0.0	0.0	7.870260332471277E-5	0.001023133843221266
18	0.0	0.0	0.0	7.870260332471277E-5	0.001023133843221266
19	0.0	0.0	0.0	7.870260332471277E-5	0.001023133843221266
20	0.0	0.0	0.0	7.870260332471277E-5	0.0011805390498706915
21	0.0	0.0	0.0	7.870260332471277E-5	0.0011805390498706915
22	0.0	0.0	0.0	7.870260332471277E-5	0.0011805390498706915
23	0.0	0.0	0.0	7.870260332471277E-5	0.0011805390498706915
24	0.0	0.0	0.0	2.3610780997413832E-4	0.0011805390498706915
25	0.0	0.0	0.0	2.3610780997413832E-4	0.0011805390498706915
26	0.0	0.0	0.0	2.3610780997413832E-4	0.0011805390498706915
27	0.0	0.0	0.0	2.3610780997413832E-4	0.0011805390498706915
28	0.0	0.0	0.0	5.509182232729895E-4	0.0011805390498706915
29	0.0	0.0	0.0	0.0011805390498706915	0.0011805390498706915
30	0.0	0.0	0.0	0.0029906989263390853	0.0011805390498706915
31	0.0	0.0	0.0	0.00708323429922415	0.0011805390498706915
32	0.0	0.0	0.0	0.01219890351533048	0.0011805390498706915
33	0.0	0.0	0.0	0.018495111781307503	0.0011805390498706915
34	0.0	0.0	0.0	0.025971859097155214	0.0011805390498706915
35	0.0	0.0	0.0	0.033684714222977065	0.0011805390498706915
36	0.0	0.0	0.0	0.044781781291761566	0.0011805390498706915
37	0.0	0.0	0.0	0.06508705294953747	0.0011805390498706915
38	0.0	0.0	0.0	0.09160983026996566	0.0012592416531954044
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1935	0.0	23.87554	1
GTACGTA	50	0.0025819575	21.997875	11
GTATAAA	185	0.0	20.215855	1
TAGGACG	255	0.0	18.979305	4
ACGTAGT	70	8.128914E-4	18.85532	13
AACGCAG	2455	0.0	18.45923	6
CGCAATA	240	0.0	18.334448	36
CGTAGTC	60	0.0074164364	18.331562	14
GGATAGT	125	4.855865E-7	17.598991	6
TCAACGC	2685	0.0	16.632195	4
CGTTTAG	120	6.398559E-6	16.502302	29
ATCAACG	2710	0.0	16.478113	3
CAACGCA	2735	0.0	16.408566	5
TATCAAC	2790	0.0	16.399849	2
TAAGACT	205	1.05501385E-10	16.09664	4
TAGCGTA	260	0.0	16.076002	7
CGTATAT	265	0.0	15.772682	10
ATAGCGT	265	0.0	15.772682	6
CGTAAAC	140	1.5865808E-6	15.7140045	40
AAGACGG	550	0.0	15.599108	5
>>END_MODULE