FastQCFastQC Report
Thu 2 Feb 2017
SRR4064048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44244
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG2310.522104692161649No Hit
GTATCAACGCAGAGTACTTTTTTTT1110.25088147545429884No Hit
TATCAACGCAGAGTACTTTTTTTTT750.16951451044209384No Hit
TCCTAACACTCCTCGTCCCCATTCT620.14013199529879758No Hit
GTATCAACGCAGAGTACATGGGGTG540.12205044751830757No Hit
GTATAGGGGTCCTAGGAAGATAATA520.11753006057318507No Hit
GTATCAACGCAGAGTACATGGGGAG510.11526986710062381No Hit
GTATCAACGCAGAGTACATGGGGGG500.11300967362806255No Hit
GTGTAGGGACACATCAGTGCATAGC490.1107494801555013No Hit
GGTATCAACGCAGAGTACATGGGGA490.1107494801555013No Hit
CATCTAGCCTATCAGTTTACTCCAT450.10170870626525631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1100.016.3778291
ACTTTTT350.002120497216.29154416
AGTACTT400.00516000314.25510213
GAGTACT400.00516000314.25510212
TACTTTT400.00516000314.25510215
CTTTTTT400.00516000314.25510217
TATCAAC1501.0913936E-1112.0240092
ATCAACG1552.1827873E-1111.6361383
TCAACGC1603.8198777E-1111.2725094
AACGCAG1709.094947E-1211.1804726
CAACGCA1709.094947E-1211.167815
ACGCAGA1751.6370905E-1110.861037
CAGAGTA1751.6370905E-1110.8610310
AGAGTAC1751.6370905E-1110.8610311
CGCAGAG1751.6370905E-1110.861038
GCAGAGT1802.910383E-1110.5593369
AGTACAT1409.577634E-79.50340113
GAGTACA1409.577634E-79.50340112
CATGGGG1000.0027820538.55306117
ACATGGG2205.5025946E-56.479591416
Produced by FastQC (version 0.11.5)