##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064048_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44244
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326733568393454	32.0	32.0	32.0	32.0	32.0
2	30.99037157580689	32.0	32.0	32.0	32.0	32.0
3	30.93895217430612	32.0	32.0	32.0	32.0	32.0
4	30.996926136877317	32.0	32.0	32.0	32.0	32.0
5	30.965690263086522	32.0	32.0	32.0	32.0	32.0
6	34.599561522466324	36.0	36.0	36.0	32.0	36.0
7	34.50137871801826	36.0	36.0	36.0	32.0	36.0
8	34.50619293011482	36.0	36.0	36.0	32.0	36.0
9	34.60663592803544	36.0	36.0	36.0	32.0	36.0
10	34.313692252056775	36.0	36.0	36.0	32.0	36.0
11	34.632424735557365	36.0	36.0	36.0	32.0	36.0
12	34.4485579965645	36.0	36.0	36.0	32.0	36.0
13	34.487455926227284	36.0	36.0	36.0	32.0	36.0
14	34.44688545339481	36.0	36.0	36.0	32.0	36.0
15	34.3753955338577	36.0	36.0	36.0	32.0	36.0
16	34.397274206672094	36.0	36.0	36.0	32.0	36.0
17	34.319274929934004	36.0	36.0	36.0	32.0	36.0
18	34.32040502667029	36.0	36.0	36.0	32.0	36.0
19	34.31342102884007	36.0	36.0	36.0	32.0	36.0
20	34.29393816110659	36.0	36.0	36.0	32.0	36.0
21	34.329943043124494	36.0	36.0	36.0	32.0	36.0
22	34.3349832745683	36.0	36.0	36.0	32.0	36.0
23	34.23506012114637	36.0	36.0	36.0	32.0	36.0
24	34.22391736732664	36.0	36.0	36.0	32.0	36.0
25	33.83505108037248	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	1.0
8	2.0
9	5.0
10	7.0
11	2.0
12	3.0
13	5.0
14	1.0
15	7.0
16	13.0
17	16.0
18	11.0
19	24.0
20	36.0
21	53.0
22	83.0
23	132.0
24	168.0
25	286.0
26	467.0
27	559.0
28	814.0
29	1098.0
30	1395.0
31	2012.0
32	2751.0
33	3955.0
34	9008.0
35	21328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99099934417332	19.72229132273457	13.727130871344897	24.559578461747215
2	15.337145957187099	21.851759759488235	39.13515224123511	23.67594204208956
3	17.634428045114483	26.112605383902537	30.334742219108108	25.918224351874876
4	11.936621310067357	18.3264771032051	38.698521766647076	31.038379820080465
5	12.922110212015731	38.68948058406039	34.833416210840376	13.554992993083495
6	28.743557283660365	39.483678451939596	19.1246948186997	12.648069445700335
7	28.358276438938372	32.2625129990505	22.338020527196274	17.04119003481485
8	27.460202604920404	32.94591172214182	21.422756874095512	18.171128798842258
9	27.65100974694136	15.314683733236844	20.317058278115745	36.71724824170605
10	14.772007599746676	29.458065683524836	33.956844295666336	21.813082421062155
11	32.58622639375777	24.523351803686534	24.132081872667648	18.758339929888045
12	22.737246917769482	27.490102929532856	30.82230516909852	18.95034498359914
13	28.80182768214617	22.38232034201181	26.57829853649709	22.23755343934493
14	22.490668476416694	21.230630019228595	28.397240131206875	27.881461373147836
15	24.247359254484177	29.080997941688718	24.107122661781005	22.564520142046096
16	22.763050755451008	27.763955487198043	26.63982629150457	22.833167465846376
17	22.08273958969487	28.90683314107349	26.934473321119178	22.075953948112463
18	22.11085972850679	29.00678733031674	27.665158371040725	21.217194570135746
19	24.286231392244694	26.406044975340482	27.90145242296729	21.406271209447535
20	24.35178062355763	26.600751165211094	27.073623240870624	21.973844970360652
21	24.269296896208488	26.712514704551626	27.28033662112026	21.737851778119627
22	24.40497737556561	26.64932126696832	27.762443438914026	21.183257918552037
23	24.11077925604127	27.35315413159562	26.375690107702056	22.160376504661055
24	22.525396502183305	28.389782574266388	26.9983483789226	22.086472544627707
25	23.988145064591297	26.897581502680936	27.524264156919525	21.59000927580824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	6.0
3	9.0
4	9.0
5	9.0
6	16.5
7	24.0
8	24.0
9	24.0
10	57.5
11	91.0
12	91.0
13	91.0
14	108.5
15	126.0
16	126.0
17	126.0
18	329.5
19	533.0
20	533.0
21	533.0
22	822.5
23	1112.0
24	1112.0
25	1112.0
26	1640.0
27	2168.0
28	2168.0
29	2168.0
30	2624.5
31	3081.0
32	3081.0
33	3081.0
34	3669.0
35	4257.0
36	4257.0
37	4257.0
38	4833.0
39	5409.0
40	5409.0
41	5409.0
42	5608.0
43	5807.0
44	5807.0
45	5807.0
46	5848.0
47	5889.0
48	5889.0
49	5889.0
50	5689.0
51	5489.0
52	5489.0
53	5489.0
54	4744.0
55	3999.0
56	3999.0
57	3999.0
58	3301.0
59	2603.0
60	2603.0
61	2603.0
62	2172.5
63	1742.0
64	1742.0
65	1742.0
66	1322.5
67	903.0
68	903.0
69	903.0
70	628.5
71	354.0
72	354.0
73	354.0
74	303.5
75	253.0
76	253.0
77	253.0
78	173.0
79	93.0
80	93.0
81	93.0
82	69.0
83	45.0
84	45.0
85	45.0
86	26.0
87	7.0
88	7.0
89	7.0
90	5.5
91	4.0
92	4.0
93	4.0
94	3.5
95	3.0
96	3.0
97	3.0
98	121.5
99	240.0
100	240.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056504836814031276
2	0.011300967362806256
3	0.002260193472561251
4	0.004520386945122502
5	0.004520386945122502
6	0.01808154778049001
7	0.02260193472561251
8	0.04520386945122502
9	0.056504836814031276
10	0.07232619112196004
11	0.0655456107042763
12	0.0881475454298888
13	0.0791067715396438
14	0.0881475454298888
15	0.07458638459452128
16	0.07232619112196004
17	0.07458638459452128
18	0.09944851279269505
19	0.09492812584757254
20	0.10396889973781755
21	0.09040773890245005
22	0.09944851279269505
23	0.10848928668294007
24	0.10170870626525631
25	0.0971883193201338
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	44244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.22366874604466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.88146279949559	23.86086248982913
2	16.694994243105434	13.76457824789802
3	8.229617851855913	10.177651206943313
4	5.181205109929272	8.54353132628153
5	3.0429299851965568	6.272036886357473
6	2.4453095016174133	6.048277732573908
7	1.4693788036624815	4.2401229545249075
8	0.964965184494764	3.1823524093662416
9	0.8553100498930863	3.173311635475997
>10	3.1964471736389055	19.299792062200524
>50	0.02741378365041943	0.6644968809330078
>100	0.010965513460167772	0.772986167615948
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	231	0.522104692161649	No Hit
GTATCAACGCAGAGTACTTTTTTTT	111	0.25088147545429884	No Hit
TATCAACGCAGAGTACTTTTTTTTT	75	0.16951451044209384	No Hit
TCCTAACACTCCTCGTCCCCATTCT	62	0.14013199529879758	No Hit
GTATCAACGCAGAGTACATGGGGTG	54	0.12205044751830757	No Hit
GTATAGGGGTCCTAGGAAGATAATA	52	0.11753006057318507	No Hit
GTATCAACGCAGAGTACATGGGGAG	51	0.11526986710062381	No Hit
GTATCAACGCAGAGTACATGGGGGG	50	0.11300967362806255	No Hit
GTGTAGGGACACATCAGTGCATAGC	49	0.1107494801555013	No Hit
GGTATCAACGCAGAGTACATGGGGA	49	0.1107494801555013	No Hit
CATCTAGCCTATCAGTTTACTCCAT	45	0.10170870626525631	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	110	0.0	16.377829	1
ACTTTTT	35	0.0021204972	16.291544	16
AGTACTT	40	0.005160003	14.255102	13
GAGTACT	40	0.005160003	14.255102	12
TACTTTT	40	0.005160003	14.255102	15
CTTTTTT	40	0.005160003	14.255102	17
TATCAAC	150	1.0913936E-11	12.024009	2
ATCAACG	155	2.1827873E-11	11.636138	3
TCAACGC	160	3.8198777E-11	11.272509	4
AACGCAG	170	9.094947E-12	11.180472	6
CAACGCA	170	9.094947E-12	11.16781	5
ACGCAGA	175	1.6370905E-11	10.86103	7
CAGAGTA	175	1.6370905E-11	10.86103	10
AGAGTAC	175	1.6370905E-11	10.86103	11
CGCAGAG	175	1.6370905E-11	10.86103	8
GCAGAGT	180	2.910383E-11	10.559336	9
AGTACAT	140	9.577634E-7	9.503401	13
GAGTACA	140	9.577634E-7	9.503401	12
CATGGGG	100	0.002782053	8.553061	17
ACATGGG	220	5.5025946E-5	6.4795914	16
>>END_MODULE