FastQCFastQC Report
Thu 2 Feb 2017
SRR4064048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44244
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT460.10396889973781755No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT460.10396889973781755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTGC207.774511E-444.019
CAATTTG207.774511E-444.018
ATGTCTT207.774511E-444.011
CTTCTCA301.2809587E-436.66666429
GGTATCA505.7278157E-835.21
CTCAAGG250.002328431735.232
CTTCAAT300.00568609929.33333215
CCTGTAA300.00568609929.33333242
TTCTTCT406.924802E-427.527
TTCTCAA406.924802E-427.530
TTTGCTT406.924802E-427.521
GTATCAA1151.2732926E-1124.8695661
TTTCTTC450.001377042224.44444526
GAGTACT450.001377042224.44444512
ATGGGGT602.8126198E-422.05
ACTTTTT500.002541676222.016
AGTACTT500.002541676222.013
GTCTTCA500.002541676222.013
TCTTCTC500.002541676222.028
CTTTTTT500.002541676222.017
Produced by FastQC (version 0.11.5)