##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44244
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36332610071422	32.0	32.0	32.0	32.0	32.0
2	31.483432781846126	32.0	32.0	32.0	32.0	32.0
3	31.526805894584577	32.0	32.0	32.0	32.0	32.0
4	31.62410722357834	32.0	32.0	32.0	32.0	32.0
5	31.57840611156315	32.0	32.0	32.0	32.0	32.0
6	35.167638549859866	36.0	36.0	36.0	36.0	36.0
7	35.18811590272127	36.0	36.0	36.0	36.0	36.0
8	35.169469306572644	36.0	36.0	36.0	36.0	36.0
9	35.238292197812136	36.0	36.0	36.0	36.0	36.0
10	35.103833288129465	36.0	36.0	36.0	36.0	36.0
11	35.25881475454299	36.0	36.0	36.0	36.0	36.0
12	35.17105144200344	36.0	36.0	36.0	36.0	36.0
13	35.18752825241841	36.0	36.0	36.0	36.0	36.0
14	35.11908959406925	36.0	36.0	36.0	36.0	36.0
15	35.11217340204321	36.0	36.0	36.0	36.0	36.0
16	35.13558900641895	36.0	36.0	36.0	36.0	36.0
17	35.09153783563873	36.0	36.0	36.0	36.0	36.0
18	35.1024545701112	36.0	36.0	36.0	36.0	36.0
19	35.087514691257574	36.0	36.0	36.0	36.0	36.0
20	35.095741795497695	36.0	36.0	36.0	36.0	36.0
21	35.07343368592352	36.0	36.0	36.0	36.0	36.0
22	35.07205496790525	36.0	36.0	36.0	36.0	36.0
23	35.002395805080916	36.0	36.0	36.0	36.0	36.0
24	35.01643160654552	36.0	36.0	36.0	36.0	36.0
25	35.00890516228189	36.0	36.0	36.0	36.0	36.0
26	34.91849742337944	36.0	36.0	36.0	36.0	36.0
27	34.89752282795407	36.0	36.0	36.0	36.0	36.0
28	34.94552933731127	36.0	36.0	36.0	36.0	36.0
29	34.92179730584938	36.0	36.0	36.0	36.0	36.0
30	34.90016725431697	36.0	36.0	36.0	36.0	36.0
31	34.904936262544076	36.0	36.0	36.0	36.0	36.0
32	34.87238947653919	36.0	36.0	36.0	32.0	36.0
33	34.83358195461531	36.0	36.0	36.0	36.0	36.0
34	34.81357924238315	36.0	36.0	36.0	32.0	36.0
35	34.75298345538378	36.0	36.0	36.0	32.0	36.0
36	34.76475906337583	36.0	36.0	36.0	32.0	36.0
37	34.701948286773344	36.0	36.0	36.0	32.0	36.0
38	34.66849742337944	36.0	36.0	36.0	32.0	36.0
39	34.67229454841335	36.0	36.0	36.0	32.0	36.0
40	34.651161739444895	36.0	36.0	36.0	32.0	36.0
41	34.64935358466685	36.0	36.0	36.0	32.0	36.0
42	34.644765391917545	36.0	36.0	36.0	32.0	36.0
43	34.577298616761595	36.0	36.0	36.0	32.0	36.0
44	34.535462435584485	36.0	36.0	36.0	32.0	36.0
45	34.51792333423741	36.0	36.0	36.0	32.0	36.0
46	34.48718470301058	36.0	36.0	36.0	32.0	36.0
47	34.474301600216975	36.0	36.0	36.0	32.0	36.0
48	34.41625983184161	36.0	36.0	36.0	32.0	36.0
49	34.415287948648405	36.0	36.0	36.0	32.0	36.0
50	33.8174215712865	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	9.0
23	14.0
24	38.0
25	79.0
26	177.0
27	321.0
28	474.0
29	753.0
30	980.0
31	1421.0
32	1904.0
33	3118.0
34	6297.0
35	28657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06419674947445	20.601731503876668	13.75709215850268	25.576979588146205
2	14.162372299068801	22.642618208118613	39.30476448784016	23.890245004972428
3	16.169424102703193	27.3099177289576	30.97821173492451	25.5424464334147
4	11.086248982912936	19.331434770816383	38.45493174215713	31.127384504113554
5	12.19148359099539	38.916011210559624	35.37202784558358	13.520477352861404
6	28.19365337672905	40.30829038965735	18.93590091311816	12.562155320495435
7	27.29409637464967	32.96040141036073	22.28550763945394	17.459994575535664
8	26.195642346984904	33.88256034716572	21.76792333423741	18.15387397161197
9	27.73257390832655	15.170418587831119	20.188048096917097	36.908959406925234
10	14.207576168520026	29.8413344182262	34.34363981556821	21.607449597685562
11	32.24844046650393	24.76493987885363	24.177289575987704	18.80933007865473
12	22.330711508905164	28.03770002712232	30.085435313262813	19.5461531507097
13	28.458096013018714	22.095651387758792	27.104240122954526	22.342012476267968
14	22.18379893318868	21.218696320405027	28.43323388482054	28.16427086158575
15	23.692418968401068	29.636996519144702	24.031463315401655	22.639121197052575
16	23.043193273512195	28.135524263725337	25.9340460637841	22.88723639897837
17	22.17927854624356	29.2288219871621	26.921164451677065	21.670735014917277
18	21.880933007865476	28.132628152969897	28.225296085344905	21.761142753819726
19	23.44724708435042	26.776512069433146	28.184612602838804	21.591628243377635
20	23.70425623290613	27.149024660382903	27.24395922334486	21.90275988336611
21	24.611246722719464	26.530150980923967	26.79007323026851	22.068529066088058
22	23.655184883826056	27.264713859506372	27.630865202061294	21.449236054606274
23	23.671006238133984	27.343820631046018	26.887261549588644	22.09791158123135
24	22.748847301328993	27.74613506916192	27.011572190579514	22.49344543892957
25	23.17533509640379	27.632738862141455	27.497118057910086	21.694807983544674
26	22.553229962479094	27.33601555083405	27.948555671081778	22.16219881560508
27	23.359479192567644	26.994281064219354	27.473496236352542	22.172743506860463
28	21.80005876635852	27.18848179373008	28.110661573582263	22.900797866329135
29	22.44648871007843	27.27663133151007	27.355739891056214	22.92114006735529
30	22.725012431626055	26.87943583020659	27.31115229872067	23.084399439446678
31	22.718231544686045	26.777722526106412	28.12033814022874	22.3837077889788
32	22.672513165924553	27.868815405826908	26.942115136857808	22.516556291390728
33	22.693368292572668	27.600470141494508	27.812937932281546	21.89322363365128
34	23.038672784395274	26.469724024139413	27.999909590217666	22.491693601247658
35	22.84758481951131	27.653081982776158	27.700549264257134	21.798783933455393
36	22.766726943942135	26.774412296564194	27.655967450271245	22.802893309222423
37	23.407092647424452	26.31828763872251	27.473272608096195	22.801347105756843
38	22.39726956285882	27.281768455313955	27.71574521947471	22.605216762352516
39	22.174001491828847	27.225876449447345	27.354716213467146	23.245405845256663
40	22.008001265736954	27.174920326379315	27.871075650385375	22.94600275749836
41	22.63234031011256	26.280457483838887	28.78034446905655	22.306857736991997
42	23.33250457699523	25.913703862757952	27.484573830888504	23.269217729358317
43	21.779716565332368	26.14424880772099	28.52880681689759	23.547227810049048
44	22.174758159298435	26.955067353765482	27.94955248169243	22.920622005243647
45	22.482144471566766	26.726787813036797	27.54271765663141	23.24835005876503
46	22.256125124310643	26.211463701292832	28.329264985082723	23.203146189313806
47	22.200122053206155	27.421286983251587	27.690256085708477	22.688334877833782
48	21.11520466514477	27.251768641366997	28.15812670930995	23.474899984178286
49	21.780208851317752	26.49066497897925	28.391573617829213	23.337552551873785
50	21.25711960943857	26.81945574541181	29.045746315884642	22.877678329264985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	4.0
6	7.0
7	7.5
8	8.0
9	9.5
10	11.0
11	17.0
12	23.0
13	38.0
14	53.0
15	78.0
16	103.0
17	95.0
18	87.0
19	75.0
20	63.0
21	110.5
22	158.0
23	243.5
24	329.0
25	473.5
26	618.0
27	705.0
28	792.0
29	855.5
30	919.0
31	1166.5
32	1414.0
33	1545.0
34	1676.0
35	1915.5
36	2155.0
37	2363.0
38	2571.0
39	2989.0
40	3407.0
41	3611.5
42	3816.0
43	3661.5
44	3507.0
45	3483.5
46	3460.0
47	3570.5
48	3681.0
49	3463.0
50	3245.0
51	3034.0
52	2823.0
53	2511.5
54	2200.0
55	2141.5
56	2083.0
57	1777.0
58	1471.0
59	1308.0
60	1145.0
61	1009.0
62	873.0
63	695.0
64	517.0
65	389.0
66	261.0
67	235.5
68	210.0
69	161.0
70	112.0
71	141.5
72	171.0
73	177.0
74	183.0
75	119.0
76	55.0
77	34.5
78	14.0
79	16.0
80	18.0
81	10.0
82	2.0
83	1.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011300967362806256
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.004520386945122502
16	0.002260193472561251
17	0.0
18	0.0
19	0.0
20	0.006780580417683754
21	0.0
22	0.0
23	0.0
24	0.0
25	0.006780580417683754
26	0.004520386945122502
27	0.011300967362806256
28	0.002260193472561251
29	0.002260193472561251
30	0.004520386945122502
31	0.004520386945122502
32	0.002260193472561251
33	0.004520386945122502
34	0.002260193472561251
35	0.006780580417683754
36	0.009040773890245005
37	0.002260193472561251
38	0.004520386945122502
39	0.006780580417683754
40	0.002260193472561251
41	0.004520386945122502
42	0.002260193472561251
43	0.002260193472561251
44	0.0
45	0.0
46	0.0
47	0.002260193472561251
48	0.002260193472561251
49	0.004520386945122502
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	44244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.38821083084712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.014032352367956	29.231082180634658
2	15.396608848177742	14.284422746587108
3	7.225687000584681	10.055600759425007
4	4.487429351003703	8.32655275291565
5	2.8064704735918924	6.509357200976404
6	1.8466185928668875	5.139679956604285
7	1.2034691093354122	3.9078745140584035
8	0.794192165269928	2.9472922882198715
9	0.7552134086922627	3.1529698942229456
>10	2.47027869810953	16.445167706355665
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT	46	0.10396889973781755	No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT	46	0.10396889973781755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.002260193472561251	0.0
26	0.0	0.0	0.0	0.002260193472561251	0.0
27	0.0	0.0	0.0	0.002260193472561251	0.0
28	0.0	0.0	0.0	0.0067805804176837535	0.0
29	0.0	0.0	0.0	0.029382515143296265	0.0
30	0.0	0.0	0.0	0.06554561070427628	0.0
31	0.0	0.0	0.0	0.13787180182623632	0.0
32	0.0	0.0	0.0	0.22375915378356387	0.0
33	0.0	0.0	0.0	0.30060573185064643	0.0
34	0.0	0.0	0.0	0.40457463158846396	0.0
35	0.0	0.0	0.0	0.49724256396347527	0.0
36	0.0	0.0	0.0	0.6464153331525179	0.0
37	0.0	0.0	0.0	0.8385317783202242	0.0
38	0.0	0.0	0.0	1.114275381972697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTGC	20	7.774511E-4	44.0	19
CAATTTG	20	7.774511E-4	44.0	18
ATGTCTT	20	7.774511E-4	44.0	11
CTTCTCA	30	1.2809587E-4	36.666664	29
GGTATCA	50	5.7278157E-8	35.2	1
CTCAAGG	25	0.0023284317	35.2	32
CTTCAAT	30	0.005686099	29.333332	15
CCTGTAA	30	0.005686099	29.333332	42
TTCTTCT	40	6.924802E-4	27.5	27
TTCTCAA	40	6.924802E-4	27.5	30
TTTGCTT	40	6.924802E-4	27.5	21
GTATCAA	115	1.2732926E-11	24.869566	1
TTTCTTC	45	0.0013770422	24.444445	26
GAGTACT	45	0.0013770422	24.444445	12
ATGGGGT	60	2.8126198E-4	22.0	5
ACTTTTT	50	0.0025416762	22.0	16
AGTACTT	50	0.0025416762	22.0	13
GTCTTCA	50	0.0025416762	22.0	13
TCTTCTC	50	0.0025416762	22.0	28
CTTTTTT	50	0.0025416762	22.0	17
>>END_MODULE