FastQCFastQC Report
Thu 2 Feb 2017
SRR4064047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173129
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28820.24566778248598406No Hit
TATCAACGCAGAGTACTTTTTTTTT19180.1634943812658284No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13170.11226386867940355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG801.9981562E-613.062537
GAACCGC1102.743036E-912.9545756
CGGAATT604.0847334E-412.66885715
CCGTCGT1301.9826984E-1012.4231059
AGAACCG1301.9826984E-1012.4209865
CGTGCAA701.0900035E-412.21483311
TTAAGGT550.003071543312.0888744
CGTCGTA1302.6102498E-911.69283210
AATACTA658.032179E-411.690345
TACCGTC1552.5465852E-1111.6451877
GATATAC2050.011.5858861
TTAGCTC1251.8339051E-811.3980824
CAGTACT855.329176E-511.1745894
ATAGAAC1701.0913936E-1111.173163
GTATCAA17800.010.9415021
CACGCCC700.001488781310.85901916
TAAATCT700.001493024810.8553154
CTAAGAC700.001494618810.8539273
TAGAACT1158.826737E-710.7373234
ATACCGT1605.4387783E-1010.6875246
Produced by FastQC (version 0.11.5)