##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064047_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173129
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243567416712057	32.0	32.0	32.0	32.0	32.0
2	30.822330707023696	32.0	32.0	32.0	32.0	32.0
3	30.830442346920073	32.0	32.0	32.0	32.0	32.0
4	30.866184366766145	32.0	32.0	32.0	32.0	32.0
5	30.758232044387274	32.0	32.0	32.0	32.0	32.0
6	34.43111797594297	36.0	36.0	36.0	32.0	36.0
7	34.3611682943649	36.0	36.0	36.0	32.0	36.0
8	34.317244736086145	36.0	36.0	36.0	32.0	36.0
9	34.46924336539289	36.0	36.0	36.0	32.0	36.0
10	34.14846363869617	36.0	36.0	36.0	32.0	36.0
11	34.446116326508	36.0	36.0	36.0	32.0	36.0
12	34.244787231412744	36.0	36.0	36.0	32.0	36.0
13	34.34146457891673	36.0	36.0	36.0	32.0	36.0
14	34.226422669629684	36.0	36.0	36.0	32.0	36.0
15	34.16755190605637	36.0	36.0	36.0	32.0	36.0
16	34.166311633247496	36.0	36.0	36.0	32.0	36.0
17	34.09548992480793	36.0	36.0	36.0	32.0	36.0
18	34.089503370899536	36.0	36.0	36.0	32.0	36.0
19	34.09482333144948	36.0	36.0	36.0	32.0	36.0
20	34.07268169144229	36.0	36.0	36.0	32.0	36.0
21	34.06712731506936	36.0	36.0	36.0	32.0	36.0
22	34.040012649930226	36.0	36.0	36.0	32.0	36.0
23	33.985310225900136	36.0	36.0	36.0	32.0	36.0
24	33.96136230542421	36.0	36.0	36.0	32.0	36.0
25	33.563159720712726	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	10.0
5	20.0
6	49.0
7	12.0
8	58.0
9	83.0
10	107.0
11	24.0
12	70.0
13	76.0
14	119.0
15	209.0
16	331.0
17	438.0
18	563.0
19	788.0
20	1166.0
21	1733.0
22	2707.0
23	4299.0
24	6464.0
25	9530.0
26	13492.0
27	17867.0
28	24224.0
29	32859.0
30	43416.0
31	58314.0
32	81532.0
33	114005.0
34	240667.0
35	517893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31736610690981	17.344446604789827	11.298919031392092	26.039268256908272
2	16.671071071753726	19.736792751997324	37.82091063774649	25.771225538502456
3	19.083953197750244	23.79401349920638	28.558800313015194	28.56323299002818
4	12.684439208478853	15.309146923107742	36.01237082116099	35.99404304725241
5	14.56672205063328	36.66671497489819	33.62798512902134	15.138577845447191
6	34.05886184295779	35.49025391650696	16.921032170941803	13.529852069593447
7	30.009395867864423	30.319493612174043	20.99379633987463	18.6773141800869
8	27.75475398652682	33.29947983286433	19.60526988999744	19.34049629061141
9	27.5682000574819	13.952579990669852	18.52716702074039	39.95205293110786
10	15.925840950554097	26.622900875344257	31.847502629103257	25.603755544998393
11	37.11513071482913	20.903199724344038	22.796713962588573	19.184955598238258
12	24.842966860121944	23.482041413758868	29.156217693480862	22.518774032638326
13	29.762809538797747	19.54801855078991	25.129035807596544	25.5601361028158
14	23.35804592259338	19.854778350168093	25.298798833381532	31.488376893856994
15	24.878243083951286	27.77047094125133	22.233793749328314	25.117492225469075
16	25.354317275499543	25.68792783571109	24.308276288571857	24.649478600217517
17	23.785778806646668	25.971765055097464	25.513806565635456	24.728649572620412
18	24.409559656570345	24.97210316539496	26.796487899428072	23.821849278606624
19	25.698413619193534	24.600163108200793	25.4054671958015	24.29595607680417
20	25.805992024965917	24.463681517338106	25.151472753673136	24.578853704022837
21	26.620064477546745	24.066726327023495	24.478945837325146	24.834263358104607
22	25.777379657474352	24.675922963721288	24.968456075244738	24.578241303559622
23	24.321550420135804	24.537142496380493	25.65912563634642	25.482181447137283
24	24.926739504874224	24.851580848448243	25.504974888818932	24.7167047578586
25	24.725133167946876	24.66132174945658	25.61934612064112	24.994198961955426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	275.5
3	442.0
4	442.0
5	442.0
6	1159.5
7	1877.0
8	1877.0
9	1877.0
10	2041.0
11	2205.0
12	2205.0
13	2205.0
14	2567.0
15	2929.0
16	2929.0
17	2929.0
18	5167.5
19	7406.0
20	7406.0
21	7406.0
22	12081.5
23	16757.0
24	16757.0
25	16757.0
26	25548.0
27	34339.0
28	34339.0
29	34339.0
30	44034.0
31	53729.0
32	53729.0
33	53729.0
34	64843.0
35	75957.0
36	75957.0
37	75957.0
38	86312.0
39	96667.0
40	96667.0
41	96667.0
42	110833.0
43	124999.0
44	124999.0
45	124999.0
46	138627.0
47	152255.0
48	152255.0
49	152255.0
50	155091.5
51	157928.0
52	157928.0
53	157928.0
54	147141.0
55	136354.0
56	136354.0
57	136354.0
58	124917.5
59	113481.0
60	113481.0
61	113481.0
62	99045.5
63	84610.0
64	84610.0
65	84610.0
66	69183.5
67	53757.0
68	53757.0
69	53757.0
70	40650.5
71	27544.0
72	27544.0
73	27544.0
74	20991.5
75	14439.0
76	14439.0
77	14439.0
78	11494.0
79	8549.0
80	8549.0
81	8549.0
82	5922.5
83	3296.0
84	3296.0
85	3296.0
86	2544.5
87	1793.0
88	1793.0
89	1793.0
90	1270.5
91	748.0
92	748.0
93	748.0
94	538.0
95	328.0
96	328.0
97	328.0
98	479.5
99	631.0
100	631.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04066049002283636
2	0.00519976916434595
3	0.001960568701310768
4	0.003921137402621536
5	0.009035664449519192
6	0.01236010703000267
7	0.023271098063384336
8	0.03656886838531824
9	0.04995188082470044
10	0.05762367139504692
11	0.05566310269373616
12	0.0652102198479451
13	0.06648885160966952
14	0.07262628406594672
15	0.060607145505737224
16	0.0685346624284286
17	0.06410207232111728
18	0.08123573792822443
19	0.07680314782091313
20	0.08277009604229373
21	0.07995710616650001
22	0.08132098004567272
23	0.08592405438788062
24	0.0802980746362932
25	0.0788489586396722
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1173129.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.82764213754169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65037823908231	45.263431906904074
2	12.228069025213696	13.897846611960041
3	3.5360643649393952	6.028386009182687
4	1.5172514770954286	3.4488729589214215
5	0.7898995949011784	2.2444065751816655
6	0.491046477340919	1.674300809233813
7	0.33910015759581386	1.3489183683247228
8	0.23469364815988983	1.066966931966747
9	0.1758142064502434	0.8991996126165275
>10	0.8845784950711039	9.067559347880628
>50	0.0704800774269953	2.8062164205608484
>100	0.07419626783996484	8.453236278305589
>500	0.007825971105734558	3.1912049834256204
>1k	6.019977773641967E-4	0.6094531855356302
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2882	0.24566778248598406	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1918	0.1634943812658284	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1317	0.11226386867940355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.524211744829426E-5	0.0
5	0.0	0.0	0.0	8.524211744829426E-5	0.0
6	0.0	0.0	0.0	8.524211744829426E-5	0.0
7	0.0	0.0	0.0	8.524211744829426E-5	0.0
8	0.0	0.0	0.0	8.524211744829426E-5	0.0
9	0.0	0.0	0.0	1.7048423489658852E-4	0.0
10	0.0	0.0	0.0	1.7048423489658852E-4	8.524211744829426E-5
11	0.0	0.0	0.0	1.7048423489658852E-4	8.524211744829426E-5
12	0.0	0.0	0.0	1.7048423489658852E-4	8.524211744829426E-5
13	0.0	0.0	0.0	1.7048423489658852E-4	1.7048423489658852E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	80	1.9981562E-6	13.06253	7
GAACCGC	110	2.743036E-9	12.954575	6
CGGAATT	60	4.0847334E-4	12.668857	15
CCGTCGT	130	1.9826984E-10	12.423105	9
AGAACCG	130	1.9826984E-10	12.420986	5
CGTGCAA	70	1.0900035E-4	12.214833	11
TTAAGGT	55	0.0030715433	12.088874	4
CGTCGTA	130	2.6102498E-9	11.692832	10
AATACTA	65	8.032179E-4	11.69034	5
TACCGTC	155	2.5465852E-11	11.645187	7
GATATAC	205	0.0	11.585886	1
TTAGCTC	125	1.8339051E-8	11.398082	4
CAGTACT	85	5.329176E-5	11.174589	4
ATAGAAC	170	1.0913936E-11	11.17316	3
GTATCAA	1780	0.0	10.941502	1
CACGCCC	70	0.0014887813	10.859019	16
TAAATCT	70	0.0014930248	10.855315	4
CTAAGAC	70	0.0014946188	10.853927	3
TAGAACT	115	8.826737E-7	10.737323	4
ATACCGT	160	5.4387783E-10	10.687524	6
>>END_MODULE