##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173129
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332192793801877	32.0	32.0	32.0	32.0	32.0
2	31.41944065827373	32.0	32.0	32.0	32.0	32.0
3	31.489103073916	32.0	32.0	32.0	32.0	32.0
4	31.568525712006096	32.0	32.0	32.0	32.0	32.0
5	31.522696139981196	32.0	32.0	32.0	32.0	32.0
6	35.10647422406232	36.0	36.0	36.0	36.0	36.0
7	35.1269860347839	36.0	36.0	36.0	36.0	36.0
8	35.07531055834439	36.0	36.0	36.0	36.0	36.0
9	35.18293043646521	36.0	36.0	36.0	36.0	36.0
10	35.042111310861806	36.0	36.0	36.0	36.0	36.0
11	35.17691916234276	36.0	36.0	36.0	36.0	36.0
12	35.082797373519874	36.0	36.0	36.0	36.0	36.0
13	35.13183545884553	36.0	36.0	36.0	36.0	36.0
14	35.07432856915139	36.0	36.0	36.0	36.0	36.0
15	35.0415137636185	36.0	36.0	36.0	36.0	36.0
16	35.05352949249401	36.0	36.0	36.0	36.0	36.0
17	35.0191283311554	36.0	36.0	36.0	36.0	36.0
18	35.01926812822801	36.0	36.0	36.0	36.0	36.0
19	35.011235763500856	36.0	36.0	36.0	36.0	36.0
20	35.00485112890398	36.0	36.0	36.0	36.0	36.0
21	35.00233563401808	36.0	36.0	36.0	36.0	36.0
22	34.98284076175766	36.0	36.0	36.0	36.0	36.0
23	34.938724556293465	36.0	36.0	36.0	36.0	36.0
24	34.91030739159973	36.0	36.0	36.0	32.0	36.0
25	34.878427692095244	36.0	36.0	36.0	32.0	36.0
26	34.82241168703527	36.0	36.0	36.0	32.0	36.0
27	34.81219115715322	36.0	36.0	36.0	32.0	36.0
28	34.78366147286445	36.0	36.0	36.0	32.0	36.0
29	34.75452571712062	36.0	36.0	36.0	32.0	36.0
30	34.736525139179065	36.0	36.0	36.0	32.0	36.0
31	34.727448558513174	36.0	36.0	36.0	32.0	36.0
32	34.6861010170237	36.0	36.0	36.0	32.0	36.0
33	34.66765121312319	36.0	36.0	36.0	32.0	36.0
34	34.665646318520814	36.0	36.0	36.0	32.0	36.0
35	34.622673209851605	36.0	36.0	36.0	32.0	36.0
36	34.59250176238078	36.0	36.0	36.0	32.0	36.0
37	34.577861428709035	36.0	36.0	36.0	32.0	36.0
38	34.528868521705625	36.0	36.0	36.0	32.0	36.0
39	34.51681784356196	36.0	36.0	36.0	32.0	36.0
40	34.5030734045446	36.0	36.0	36.0	32.0	36.0
41	34.48825576726856	36.0	36.0	36.0	32.0	36.0
42	34.41391611664191	36.0	36.0	36.0	32.0	36.0
43	34.41323162243879	36.0	36.0	36.0	32.0	36.0
44	34.34561246035176	36.0	36.0	36.0	32.0	36.0
45	34.317102381750004	36.0	36.0	36.0	32.0	36.0
46	34.27459725230558	36.0	36.0	36.0	32.0	36.0
47	34.24631221289389	36.0	36.0	36.0	32.0	36.0
48	34.208172332284	36.0	36.0	36.0	32.0	36.0
49	34.177502218426106	36.0	36.0	36.0	32.0	36.0
50	33.58908866799815	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	3.0
20	9.0
21	36.0
22	110.0
23	312.0
24	890.0
25	2123.0
26	4482.0
27	8088.0
28	13455.0
29	20523.0
30	29875.0
31	42519.0
32	60574.0
33	97222.0
34	200475.0
35	692430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46641478036105	18.2625812435636	11.903247696536107	26.36775627953924
2	15.974925114608322	20.636743197235422	37.309376827382565	26.078954860773695
3	18.327954541967358	24.412381960676004	28.88419487761758	28.37546861973906
4	12.388577897230398	16.048874420460155	36.010362031797015	35.552185650512435
5	14.206366051815273	37.12439126472877	33.46852733160633	15.200715351849626
6	34.0072302487284	35.98629986352714	16.655997176776257	13.350472710968205
7	29.880686608207625	30.551286346173352	21.039288944353093	18.528738101265933
8	27.6905608846086	33.667056223143405	19.412187406500053	19.230195485747945
9	27.586392080544808	13.81361184065276	18.48239424913755	40.117601829664885
10	15.81916954858024	27.046996857101696	31.84172817637737	25.29210541794069
11	37.41310631652615	20.83862899987981	22.70108402400759	19.047180659586456
12	24.859179901937384	23.576199698583615	29.132967587509633	22.431652811969368
13	29.833034841307658	19.600569081227054	25.24569118733905	25.32070489012624
14	23.36428761396881	19.842762256122093	25.233563600902887	31.55938652900621
15	24.88167940232754	27.91283488650429	22.124673083939427	25.080812627228738
16	25.30913343755701	25.75265233335436	24.316991129622085	24.621223099466548
17	23.86540610623384	26.037630985168725	25.441191889383006	24.655771019214427
18	24.59068656909804	24.902248101861442	26.74272007283124	23.76434525620928
19	25.68933169327499	24.66250514649284	25.427808876943626	24.22035428328854
20	25.891235245529227	24.520359827093415	25.151156873258383	24.437248054118974
21	26.733291905664252	24.076806557505613	24.550156035695988	24.639745501134147
22	25.872327439678262	24.59394813515343	25.124070219226237	24.409654205942072
23	24.319442443605467	24.504504658095215	25.725864128021115	25.45018877027821
24	25.08505061267981	24.78789557805008	25.549025039957378	24.57802876931273
25	24.752815377231993	24.705420032716425	25.69253889870609	24.84922569134549
26	24.30421054067325	25.444309564078782	26.13788259043047	24.113597304817503
27	24.936704112826092	24.958271600307572	25.266609949005254	24.838414337861085
28	24.341242149931933	24.923470627297906	25.834236227909628	24.901050994860533
29	24.56585116358367	24.788168101611117	25.482226579149263	25.163754155655955
30	24.301649070612957	25.046522630499922	25.813730462839434	24.838097836047687
31	25.180981511413002	24.61595774426963	25.02020377682755	25.18285696748982
32	24.798801118069925	24.97559869507362	24.921894513052248	25.303705673804206
33	24.306441163433014	24.768984212500424	25.657414669076278	25.26715995499028
34	25.07503654861712	24.77812964849394	25.80413351007378	24.34270029281516
35	25.44913165748442	24.78772002656217	25.612289520815413	24.150858795137996
36	24.376302699384933	25.43662007851361	25.352906324991796	24.834170897109658
37	25.272340255039545	24.95837854933154	24.941670296733452	24.827610898895465
38	24.672132824568045	25.09113104798148	25.41627025939528	24.8204658680552
39	25.39584033061853	24.622146599447266	24.912068633351346	25.06994443658286
40	25.662094150862337	24.849031963283483	25.439776454782898	24.049097431071285
41	24.79306717495563	24.93090873132916	25.839708256683654	24.436315837031554
42	25.492920527486767	25.5606038649402	25.23770149431852	23.70877411325451
43	24.873861471381748	24.91008980364244	25.443797070193458	24.772251654782355
44	24.482614588572257	25.322859748872656	24.992200219928225	25.202325442626865
45	24.571885476967616	25.475723057277712	25.10133329468958	24.851058171065095
46	24.582011357766227	24.929631972231267	25.13242487115533	25.355931798847177
47	24.98885430811892	24.902246101991395	25.377653548421748	24.73124604146794
48	25.38080499050811	25.36929708884185	24.57260931867255	24.677288601977484
49	24.881065530101225	25.31119872136388	24.811848694725626	24.99588705380927
50	24.650609701521166	25.922948730516616	24.53698060292469	24.88946096503753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	31.5
2	34.0
3	80.5
4	127.0
5	156.0
6	185.0
7	182.0
8	179.0
9	257.0
10	335.0
11	507.5
12	680.0
13	1244.5
14	1809.0
15	2431.0
16	3053.0
17	3235.5
18	3418.0
19	3415.0
20	3412.0
21	3737.0
22	4062.0
23	4694.5
24	5327.0
25	6247.0
26	7167.0
27	9258.0
28	11349.0
29	13377.5
30	15406.0
31	18072.0
32	20738.0
33	24466.0
34	28194.0
35	32728.5
36	37263.0
37	42116.5
38	46970.0
39	49635.5
40	52301.0
41	55920.0
42	59539.0
43	61002.0
44	62465.0
45	68438.0
46	74411.0
47	78714.0
48	83017.0
49	86402.5
50	89788.0
51	87910.0
52	86032.0
53	85449.0
54	84866.0
55	84828.0
56	84790.0
57	82014.5
58	79239.0
59	71784.5
60	64330.0
61	56560.5
62	48791.0
63	42339.5
64	35888.0
65	30426.5
66	24965.0
67	21073.0
68	17181.0
69	15462.5
70	13744.0
71	10619.5
72	7495.0
73	6452.0
74	5409.0
75	4345.0
76	3281.0
77	2802.0
78	2323.0
79	1892.0
80	1461.0
81	1185.0
82	909.0
83	755.0
84	601.0
85	432.0
86	263.0
87	183.5
88	104.0
89	89.5
90	75.0
91	59.5
92	44.0
93	35.5
94	27.0
95	27.5
96	28.0
97	18.5
98	9.0
99	12.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00963235927165725
2	2.557263523448828E-4
3	2.557263523448828E-4
4	0.0
5	0.0
6	1.7048423489658852E-4
7	0.0
8	0.0
9	0.0015343581140692967
10	6.819369395863541E-4
11	0.0
12	8.524211744829426E-5
13	8.524211744829425E-4
14	8.524211744829426E-5
15	0.003835895285173242
16	9.376632919312369E-4
17	0.0
18	5.114527046897655E-4
19	0.0
20	8.524211744829425E-4
21	0.0
22	2.557263523448828E-4
23	8.524211744829425E-4
24	3.4096846979317705E-4
25	0.0015343581140692967
26	0.006222674573725481
27	0.005711221869035716
28	0.004262105872414713
29	0.002472021406000534
30	0.0037506531677249477
31	0.006904611513311835
32	0.0030687162281385935
33	0.004176863754966419
34	0.002898231993242005
35	0.0027277477583454164
36	0.007160337865656718
37	0.004773558577104479
38	0.007927516922691367
39	0.004688316459656184
40	0.0028129898757937107
41	0.0036654110502766534
42	0.0016196002315175908
43	0.0011933896442761196
44	0.0016196002315175908
45	0.001704842348965885
46	9.376632919312369E-4
47	0.002557263523448828
48	0.0018753265838624739
49	3.4096846979317705E-4
50	0.0011933896442761196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1173129.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.60729690849812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.65666525824032	48.88382458955516
2	11.843953274087673	14.356599853060198
3	3.3643642208496014	6.117150637240791
4	1.3868549922972186	3.3621412914876165
5	0.7178850297196161	2.175453557119139
6	0.46164216879808534	1.678733039389714
7	0.31996570884242637	1.3574579701445697
8	0.21133076614495255	1.0246549191718015
9	0.16461254984815515	0.8979049515160878
>10	0.7469525240054229	8.042083790215262
>50	0.060789137121963904	2.5678743990409028
>100	0.05974598386010368	7.239164077397538
>500	0.004955230174581917	2.117621126815249
>1k	2.831560099761095E-4	0.17933579784609874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.557263523448828E-4
2	0.0	0.0	0.0	0.0	2.557263523448828E-4
3	0.0	0.0	0.0	0.0	2.557263523448828E-4
4	0.0	0.0	0.0	8.524211744829426E-5	2.557263523448828E-4
5	0.0	0.0	0.0	8.524211744829426E-5	2.557263523448828E-4
6	8.524211744829426E-5	0.0	0.0	8.524211744829426E-5	2.557263523448828E-4
7	2.557263523448828E-4	0.0	0.0	8.524211744829426E-5	2.557263523448828E-4
8	2.557263523448828E-4	0.0	0.0	8.524211744829426E-5	2.557263523448828E-4
9	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	3.4096846979317705E-4
10	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	3.4096846979317705E-4
11	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	3.4096846979317705E-4
12	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	5.114527046897655E-4
13	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	5.114527046897655E-4
14	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	5.114527046897655E-4
15	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	6.819369395863541E-4
16	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	6.819369395863541E-4
17	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	6.819369395863541E-4
18	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	6.819369395863541E-4
19	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	6.819369395863541E-4
20	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	8.524211744829427E-4
21	2.557263523448828E-4	0.0	0.0	1.7048423489658852E-4	8.524211744829427E-4
22	3.4096846979317705E-4	0.0	0.0	5.114527046897655E-4	8.524211744829427E-4
23	3.4096846979317705E-4	0.0	0.0	7.671790570346484E-4	8.524211744829427E-4
24	3.4096846979317705E-4	0.0	0.0	0.001022905409379531	8.524211744829427E-4
25	3.4096846979317705E-4	0.0	0.0	0.001278631761724414	8.524211744829427E-4
26	3.4096846979317705E-4	0.0	0.0	0.0013638738791727082	9.376632919312369E-4
27	3.4096846979317705E-4	0.0	0.0	0.001960568701310768	9.376632919312369E-4
28	3.4096846979317705E-4	0.0	0.0	0.0037506531677249477	9.376632919312369E-4
29	3.4096846979317705E-4	0.0	0.0	0.008098001157587955	9.376632919312369E-4
30	3.4096846979317705E-4	0.0	0.0	0.01619600231517591	9.376632919312369E-4
31	3.4096846979317705E-4	0.0	0.0	0.033073941569938176	9.376632919312369E-4
32	3.4096846979317705E-4	0.0	0.0	0.052423902230700974	9.376632919312369E-4
33	3.4096846979317705E-4	0.0	0.0	0.07109192595187741	9.376632919312369E-4
34	3.4096846979317705E-4	0.0	0.0	0.09240245531395098	9.376632919312369E-4
35	3.4096846979317705E-4	0.0	0.0	0.1174636378437495	9.376632919312369E-4
36	3.4096846979317705E-4	0.0	0.0	0.15565210646058533	9.376632919312369E-4
37	3.4096846979317705E-4	0.0	0.0	0.20739407175169994	9.376632919312369E-4
38	3.4096846979317705E-4	0.0	0.0	0.2688536384319201	9.376632919312369E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	560	0.0	23.570835	4
GTATCAA	2155	0.0	22.259405	1
TTAGGAC	400	0.0	21.999445	3
AGGACGT	640	0.0	20.624481	5
GACGTGA	405	0.0	20.098259	7
GGACGTG	650	0.0	19.968727	6
GATATAC	245	0.0	19.758816	1
CTTTAGG	475	0.0	19.456287	1
GTAGGAC	780	0.0	19.179005	3
TGTAGGA	810	0.0	19.011866	2
TAAACGC	255	0.0	18.979914	28
TGGCGAG	790	0.0	18.657759	18
CTCTATA	95	1.5973905E-5	18.52585	2
GTAAACG	250	0.0	18.479534	27
AAACGCT	275	0.0	18.399536	29
TCCTACA	710	0.0	18.281229	2
GTATATA	145	6.2918843E-9	18.21032	1
CTGTAGG	835	0.0	17.919535	1
TAGAACA	420	0.0	17.809074	4
GTCCTAC	620	0.0	17.74527	1
>>END_MODULE