FastQCFastQC Report
Thu 2 Feb 2017
SRR4064046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597763
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22620.3784108417550099No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18060.30212642803251455No Hit
TATCAACGCAGAGTACTTTTTTTTT17810.29794416850825495No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11800.1974026495450538No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9840.16461373487485842No Hit
GTACATGGGGTGGTATCAACGCAAA8620.1442043083964715No Hit
GGTATCAACGCAGAGTACTTTTTTT7820.13082107791884073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC250.006025371619.001341
CCGATCC456.7501015E-414.7788216
GCGTTAT456.7501015E-414.778821
GGACTAT400.005271604314.2510041
ATCTTAC400.005271604314.2510041
GTGTTAG551.9558049E-413.8191551
TAGAGCG500.001497812213.2998255
GAGCGTG604.0859322E-412.6664987
GCTACAC604.0859322E-412.6664983
ATTACCG1301.9645086E-1012.42395215
TTACCGC1301.9645086E-1012.42395216
GTTCTGA1103.792229E-812.0917611
GCTATAC550.00306302812.0917611
TCTTGGA1103.814239E-812.0867022
TTAGGAC951.0363729E-611.9998423
CGCAAGA951.0405729E-611.9958252
TACCGCG1353.6925485E-1011.96380617
AATCCTT802.8598324E-511.8748436
GACCGGC658.011629E-411.6921547
ATAGACA658.011629E-411.6921544
Produced by FastQC (version 0.11.5)