##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064046_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597763
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112479360549248	32.0	32.0	32.0	32.0	32.0
2	30.668028968002368	32.0	32.0	32.0	32.0	32.0
3	30.687785961994972	32.0	32.0	32.0	32.0	32.0
4	30.722891848441606	32.0	32.0	32.0	32.0	32.0
5	30.62278695737274	32.0	32.0	32.0	32.0	32.0
6	34.241970479939376	36.0	36.0	36.0	32.0	36.0
7	34.14943882441703	36.0	36.0	36.0	32.0	36.0
8	34.0990074661697	36.0	36.0	36.0	32.0	36.0
9	34.256123246169466	36.0	36.0	36.0	32.0	36.0
10	33.91065857204276	36.0	36.0	36.0	32.0	36.0
11	34.28706360212994	36.0	36.0	36.0	32.0	36.0
12	34.05806983704244	36.0	36.0	36.0	32.0	36.0
13	34.15456794749759	36.0	36.0	36.0	32.0	36.0
14	34.04469831689148	36.0	36.0	36.0	32.0	36.0
15	33.96949627193386	36.0	36.0	36.0	32.0	36.0
16	33.977506135374725	36.0	36.0	36.0	32.0	36.0
17	33.9039585922849	36.0	36.0	36.0	32.0	36.0
18	33.89183338547217	36.0	36.0	36.0	32.0	36.0
19	33.91375177118691	36.0	36.0	36.0	32.0	36.0
20	33.882682601633086	36.0	36.0	36.0	32.0	36.0
21	33.85622730078643	36.0	36.0	36.0	32.0	36.0
22	33.85141937523734	36.0	36.0	36.0	32.0	36.0
23	33.805427903700966	36.0	36.0	36.0	32.0	36.0
24	33.7975552183725	36.0	36.0	36.0	32.0	36.0
25	33.37894115226268	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	4.0
5	8.0
6	11.0
7	12.0
8	31.0
9	41.0
10	46.0
11	20.0
12	21.0
13	26.0
14	62.0
15	123.0
16	190.0
17	239.0
18	332.0
19	446.0
20	645.0
21	998.0
22	1602.0
23	2582.0
24	3818.0
25	5557.0
26	7800.0
27	10597.0
28	14028.0
29	18853.0
30	24329.0
31	32986.0
32	45364.0
33	62090.0
34	126552.0
35	238348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.768775824760645	17.97110471820155	11.849642783626788	25.41047667341102
2	15.730972432738088	20.536924204250024	38.74975323636777	24.982350126644114
3	18.188661947915293	25.1789606813471	29.514501712234235	27.117875658503372
4	12.03010663463501	16.123846074058708	37.41614275055124	34.42990454075504
5	13.649702201699792	37.6880479154119	34.35672221106873	14.305527671819581
6	32.18933307456274	36.82461711432864	17.864038360509824	13.122011450598798
7	28.55644124969882	31.5305999518111	21.91473911064707	17.99821968784301
8	27.298772191997912	33.555455520617706	20.38208139980488	18.76369088757951
9	27.442267589180215	14.207913666656625	19.328098169366257	39.0217205747969
10	15.208829540262014	27.652904264631793	33.098055231492566	24.04021096361363
11	35.758604356114816	21.719649381798583	23.610155675954335	18.91159058613227
12	23.94573422827779	24.59601961736103	30.240865038581926	21.217381115779254
13	29.129109525411646	20.393423085485495	25.91865395205742	24.558813437045444
14	22.849280122130658	20.35644876990524	26.91293671000497	29.88133439795913
15	24.26891138893027	28.040058182451194	23.668842092224875	24.02218833639366
16	24.092769440654845	26.543241908619926	25.438445249792853	23.92554340093238
17	22.800034147574433	26.45951587443736	26.722150895453183	24.018299082535023
18	23.28760701932961	25.917177000010046	27.67502285199608	23.120193128664273
19	24.612330104076165	25.67502188830353	26.743572874239767	22.969075133380542
20	24.861798907784465	25.17637171489893	26.52121650444152	23.440612872875086
21	25.64243359705895	24.861636125610627	26.00420532428876	23.49172495304166
22	24.605365588247604	25.200351567404677	26.418114091993473	23.776168752354245
23	23.589595056362622	25.227986934385637	26.773292169839593	24.409125839412148
24	23.721481521164108	25.876062223353173	26.70039442052326	23.702061834959455
25	23.853043594723097	25.706488984129113	26.56214424429117	23.878323176856625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	260.0
1	260.0
2	389.0
3	518.0
4	518.0
5	518.0
6	1414.5
7	2311.0
8	2311.0
9	2311.0
10	2286.0
11	2261.0
12	2261.0
13	2261.0
14	2287.5
15	2314.0
16	2314.0
17	2314.0
18	4025.5
19	5737.0
20	5737.0
21	5737.0
22	8940.0
23	12143.0
24	12143.0
25	12143.0
26	17379.0
27	22615.0
28	22615.0
29	22615.0
30	27853.5
31	33092.0
32	33092.0
33	33092.0
34	38615.5
35	44139.0
36	44139.0
37	44139.0
38	49028.5
39	53918.0
40	53918.0
41	53918.0
42	59792.0
43	65666.0
44	65666.0
45	65666.0
46	72319.5
47	78973.0
48	78973.0
49	78973.0
50	79071.0
51	79169.0
52	79169.0
53	79169.0
54	71429.0
55	63689.0
56	63689.0
57	63689.0
58	56753.0
59	49817.0
60	49817.0
61	49817.0
62	42923.0
63	36029.0
64	36029.0
65	36029.0
66	29281.5
67	22534.0
68	22534.0
69	22534.0
70	16808.0
71	11082.0
72	11082.0
73	11082.0
74	8356.0
75	5630.0
76	5630.0
77	5630.0
78	4460.0
79	3290.0
80	3290.0
81	3290.0
82	2243.5
83	1197.0
84	1197.0
85	1197.0
86	931.5
87	666.0
88	666.0
89	666.0
90	467.5
91	269.0
92	269.0
93	269.0
94	190.5
95	112.0
96	112.0
97	112.0
98	222.0
99	332.0
100	332.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037807626099306914
2	0.004182259524259615
3	0.0010037422858223076
4	0.003513098000378076
5	0.007193486381726537
6	0.010873874763074998
7	0.019238393811594225
8	0.03245433390825461
9	0.044331950957151915
10	0.05252917962470076
11	0.05085627581499692
12	0.05570769686313807
13	0.056878729529930755
14	0.0618974409590423
15	0.05554040648216768
16	0.06156286019710153
17	0.059555375625456916
18	0.0737750580079396
19	0.06892363695979845
20	0.07444421953182113
21	0.07109841191241345
22	0.07327318686502844
23	0.07862647905608075
24	0.07210215419823575
25	0.07410963876988037
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	597763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.41316456211259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79698279079453	51.23592365834833
2	12.650594404104487	16.04428449512037
3	3.2275466108579747	6.140068330986765
4	1.2001754634401083	3.044276966661493
5	0.5876958425994492	1.8633826589614149
6	0.32491993272950337	1.2362520698211932
7	0.21810676327388656	0.9681588050117693
8	0.14593512500169636	0.7403366477700035
9	0.11370008619766145	0.6489074049100823
>10	0.6085153689786934	7.448366544899542
>50	0.07390718742076106	3.2430536454912176
>100	0.049801223597101915	5.674920788746536
>500	0.0010596005020644115	0.5313256506168668
>1k	0.0010596005020644115	1.1807423326544282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2262	0.3784108417550099	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1806	0.30212642803251455	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1781	0.29794416850825495	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1180	0.1974026495450538	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	984	0.16461373487485842	No Hit
GTACATGGGGTGGTATCAACGCAAA	862	0.1442043083964715	No Hit
GGTATCAACGCAGAGTACTTTTTTT	782	0.13082107791884073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.6729038097038459E-4	0.0
8	0.0	0.0	0.0	5.018711429111537E-4	0.0
9	0.0	0.0	0.0	6.691615238815383E-4	0.0
10	0.0	0.0	0.0	6.691615238815383E-4	0.0
11	0.0	0.0	0.0	6.691615238815383E-4	0.0
12	0.0	0.0	0.0	0.0010037422858223074	0.0
13	0.0	0.0	0.0	0.0010037422858223074	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAC	25	0.0060253716	19.00134	1
CCGATCC	45	6.7501015E-4	14.77882	16
GCGTTAT	45	6.7501015E-4	14.77882	1
GGACTAT	40	0.0052716043	14.251004	1
ATCTTAC	40	0.0052716043	14.251004	1
GTGTTAG	55	1.9558049E-4	13.819155	1
TAGAGCG	50	0.0014978122	13.299825	5
GAGCGTG	60	4.0859322E-4	12.666498	7
GCTACAC	60	4.0859322E-4	12.666498	3
ATTACCG	130	1.9645086E-10	12.423952	15
TTACCGC	130	1.9645086E-10	12.423952	16
GTTCTGA	110	3.792229E-8	12.091761	1
GCTATAC	55	0.003063028	12.091761	1
TCTTGGA	110	3.814239E-8	12.086702	2
TTAGGAC	95	1.0363729E-6	11.999842	3
CGCAAGA	95	1.0405729E-6	11.995825	2
TACCGCG	135	3.6925485E-10	11.963806	17
AATCCTT	80	2.8598324E-5	11.874843	6
GACCGGC	65	8.011629E-4	11.692154	7
ATAGACA	65	8.011629E-4	11.692154	4
>>END_MODULE