FastQCFastQC Report
Thu 2 Feb 2017
SRR4064046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597763
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.1129210071550096No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.10372003620163844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGC250.00235261335.1976558
TAGACCG300.005744586729.3313835
CCCTTAC551.5930228E-423.9984041
CTATACT803.6125675E-621.9985374
GTCCTAG1004.6173227E-821.9985351
TACGACG1057.7350705E-820.9509875
CGACGGT1101.2638338E-719.998677
CGTAACA550.00448273119.9986714
CGTCGTA808.983478E-519.2487210
CGTTTAT808.983478E-519.2487231
CTACGAC1152.0185507E-719.1291624
GTCGTAG951.5958238E-518.52508411
TTTAGAC600.00741067218.3321153
CATAGGG1203.157529E-718.3321152
CCTATAC600.00741067218.3321153
ACGGTAT1203.157529E-718.3321159
TAGGACG1456.2937033E-918.2056854
ATATACT851.4300292E-418.1164424
ATACCGT851.4300292E-418.1164426
GGTTCCG1102.7889419E-618.00181443
Produced by FastQC (version 0.11.5)