FastQCFastQC Report
Thu 2 Feb 2017
SRR4064045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255285
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28750.22903165416618534No Hit
TATCAACGCAGAGTACTTTTTTTTT21070.1678503288097922No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15130.12053039747945686No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13500.10754529847803486No Hit
GTATCTGATCGTCTTCGAACCTCCG12830.10220786514616204No Hit
CTATTGGAGCTGGAATTACCGCGGC12620.10053493828094816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1004.0017767E-1115.20178615
AATGCGC400.005278426714.249969510
GCTAGAC400.005285410714.247133
CGAACGA903.6308847E-813.72383516
AACCGCG1205.0931703E-1113.4588427
AGGCTAT500.001500190613.2989126
TAGAACC2000.013.2978524
AGGCCCG2550.013.03918710
CGAACCT2900.012.44973916
AGAACCG1850.012.32282455
AGTGACG2200.012.0923319
CGACCAC550.003066095512.09184714
CGACCAT2000.011.87497510
GCGTTAT1201.0004442E-811.8749741
CGTCGTA1459.640644E-1111.79307910
GTCTTAA1052.718134E-711.761881
ACGGTAT2750.011.7458989
TCCCGAC2200.011.655352
ACCTCCG3100.011.6465319
TCTTCGA3100.011.64513712
Produced by FastQC (version 0.11.5)