##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064045_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1255285
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.190551149738905	32.0	32.0	32.0	32.0	32.0
2	30.772446894529928	32.0	32.0	32.0	32.0	32.0
3	30.79830476744325	32.0	32.0	32.0	32.0	32.0
4	30.824126791923746	32.0	32.0	32.0	32.0	32.0
5	30.726037513393372	32.0	32.0	32.0	32.0	32.0
6	34.364231230358044	36.0	36.0	36.0	32.0	36.0
7	34.30607152957297	36.0	36.0	36.0	32.0	36.0
8	34.26004692161541	36.0	36.0	36.0	32.0	36.0
9	34.40947274921631	36.0	36.0	36.0	32.0	36.0
10	34.09164213704457	36.0	36.0	36.0	32.0	36.0
11	34.38577374859096	36.0	36.0	36.0	32.0	36.0
12	34.183882544601424	36.0	36.0	36.0	32.0	36.0
13	34.27392185838276	36.0	36.0	36.0	32.0	36.0
14	34.16616704573065	36.0	36.0	36.0	32.0	36.0
15	34.0976009432121	36.0	36.0	36.0	32.0	36.0
16	34.104861445807124	36.0	36.0	36.0	32.0	36.0
17	34.03290089501587	36.0	36.0	36.0	32.0	36.0
18	34.01877661248243	36.0	36.0	36.0	32.0	36.0
19	34.045262231286124	36.0	36.0	36.0	32.0	36.0
20	34.02035553679045	36.0	36.0	36.0	32.0	36.0
21	34.00263844465599	36.0	36.0	36.0	32.0	36.0
22	33.98269078336792	36.0	36.0	36.0	32.0	36.0
23	33.924701561796724	36.0	36.0	36.0	32.0	36.0
24	33.90943251930837	36.0	36.0	36.0	32.0	36.0
25	33.50180875259403	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	12.0
5	19.0
6	50.0
7	15.0
8	71.0
9	68.0
10	94.0
11	38.0
12	61.0
13	47.0
14	104.0
15	217.0
16	338.0
17	471.0
18	588.0
19	869.0
20	1251.0
21	1926.0
22	3073.0
23	4863.0
24	7334.0
25	10666.0
26	15372.0
27	20107.0
28	26987.0
29	36511.0
30	47942.0
31	64422.0
32	90038.0
33	124605.0
34	259664.0
35	537458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.508540626349784	17.10611670462678	11.342369380296551	26.042973288726884
2	16.91751486606204	19.596592755941643	37.35827050178138	26.127621876214942
3	18.777559053549904	23.68308054353723	28.482169815153686	29.05719058775918
4	12.608416817702006	15.131390786512377	35.86137943396602	36.398812961819594
5	14.71511713599407	36.536391629785726	33.54985201148853	15.198639222731671
6	34.34337720405983	35.362556936469375	16.790093751468973	13.503972108001818
7	30.52259427828348	30.331440118310006	20.7043301716005	18.44163543180601
8	28.412083791879805	32.51433812718598	19.478974332819867	19.59460374811435
9	27.6412351480335	13.995995906643415	18.532560356287796	39.83020858903529
10	15.90195372029408	26.380006248844285	31.624492281402038	26.0935477494596
11	37.28913391575479	20.833031123495292	22.491186764085835	19.38664819666408
12	24.792497136448528	23.48957125241419	28.831519551460634	22.88641205967665
13	29.498485100901604	19.3430415475326	25.287854741861356	25.870618609704444
14	23.62256630995795	19.57497857754927	25.264462645224288	31.537992467268488
15	25.106847655409336	27.244039885540296	22.284331933141505	25.364780525908863
16	25.80024694723635	25.540545818756033	23.84252450540805	24.816682728599567
17	24.113751616107027	25.77090895757709	25.368217332319432	24.747122093996452
18	24.84954778636543	24.7849743101201	26.244334873264584	24.121143030249883
19	25.719219142612975	24.739958673996505	25.463163735156442	24.07765844823408
20	25.91883656818867	24.097426731443495	25.004584018278674	24.979152682089165
21	26.94078341619972	23.915422873673997	24.404011705724276	24.739782004402002
22	25.92812857995869	24.127157741001724	24.957767947326925	24.986945731712666
23	24.42180287484155	24.110082673618585	25.53179785223984	25.936316599300024
24	24.819294781292275	24.778956481671184	25.31212357231517	25.08962516472137
25	24.862421524663677	24.392944693572495	25.34297957149975	25.401654210264073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	89.0
2	279.5
3	470.0
4	470.0
5	470.0
6	1350.0
7	2230.0
8	2230.0
9	2230.0
10	2267.5
11	2305.0
12	2305.0
13	2305.0
14	2731.0
15	3157.0
16	3157.0
17	3157.0
18	5445.0
19	7733.0
20	7733.0
21	7733.0
22	12783.0
23	17833.0
24	17833.0
25	17833.0
26	26550.5
27	35268.0
28	35268.0
29	35268.0
30	44101.0
31	52934.0
32	52934.0
33	52934.0
34	65940.0
35	78946.0
36	78946.0
37	78946.0
38	90494.0
39	102042.0
40	102042.0
41	102042.0
42	116208.5
43	130375.0
44	130375.0
45	130375.0
46	145512.0
47	160649.0
48	160649.0
49	160649.0
50	165553.0
51	170457.0
52	170457.0
53	170457.0
54	159027.5
55	147598.0
56	147598.0
57	147598.0
58	134943.5
59	122289.0
60	122289.0
61	122289.0
62	107752.0
63	93215.0
64	93215.0
65	93215.0
66	77038.5
67	60862.0
68	60862.0
69	60862.0
70	45979.5
71	31097.0
72	31097.0
73	31097.0
74	24068.0
75	17039.0
76	17039.0
77	17039.0
78	13709.5
79	10380.0
80	10380.0
81	10380.0
82	7237.0
83	4094.0
84	4094.0
85	4094.0
86	3167.0
87	2240.0
88	2240.0
89	2240.0
90	1586.5
91	933.0
92	933.0
93	933.0
94	635.0
95	337.0
96	337.0
97	337.0
98	525.0
99	713.0
100	713.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03656540148253186
2	0.006134065172450877
3	0.0020712427855028937
4	0.004779791043468216
5	0.009559582086936433
6	0.012586783081132969
7	0.0220667019840116
8	0.0329805581999307
9	0.04532835172889026
10	0.052020059189745754
11	0.0508251114288787
12	0.05767614525784981
13	0.05942873530712149
14	0.06054401988393074
15	0.05536591292017351
16	0.061021998988277565
17	0.05791513481002322
18	0.07169686565202325
19	0.06922730694623133
20	0.07384777162158394
21	0.06819168555347989
22	0.07217484475637007
23	0.07607834077520244
24	0.07121888654767643
25	0.07249349749260128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1255285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.839908350061926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60261336283764	43.65400021243431
2	12.14729018552984	13.323125609521263
3	3.555710272960565	5.849844764655933
4	1.4052794307317196	3.082615807502188
5	0.8072824961692543	2.2135649051265562
6	0.4896631393482127	1.6111849010555754
7	0.3326072320839439	1.2768105086835881
8	0.25118219294457256	1.1019846752198348
9	0.18828480784512147	0.9292969445341933
>10	1.0524648176312041	10.422641878994822
>50	0.07758046181754157	2.957464110372539
>100	0.08057121378739703	9.13554121362701
>500	0.007284912548378491	2.7808670011285894
>1k	0.0021854737645135474	1.6610574671434963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2875	0.22903165416618534	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2107	0.1678503288097922	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1513	0.12053039747945686	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1350	0.10754529847803486	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1283	0.10220786514616204	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1262	0.10053493828094816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.966318405780361E-5
2	0.0	0.0	0.0	0.0	7.966318405780361E-5
3	0.0	0.0	0.0	0.0	7.966318405780361E-5
4	0.0	0.0	0.0	0.0	7.966318405780361E-5
5	0.0	0.0	0.0	0.0	7.966318405780361E-5
6	0.0	0.0	0.0	0.0	1.5932636811560722E-4
7	0.0	0.0	0.0	0.0	1.5932636811560722E-4
8	0.0	0.0	0.0	0.0	2.389895521734108E-4
9	0.0	0.0	0.0	0.0	2.389895521734108E-4
10	0.0	0.0	0.0	0.0	2.389895521734108E-4
11	0.0	0.0	0.0	0.0	2.389895521734108E-4
12	0.0	0.0	0.0	0.0	2.389895521734108E-4
13	0.0	0.0	0.0	0.0	2.389895521734108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	100	4.0017767E-11	15.201786	15
AATGCGC	40	0.0052784267	14.2499695	10
GCTAGAC	40	0.0052854107	14.24713	3
CGAACGA	90	3.6308847E-8	13.723835	16
AACCGCG	120	5.0931703E-11	13.458842	7
AGGCTAT	50	0.0015001906	13.298912	6
TAGAACC	200	0.0	13.297852	4
AGGCCCG	255	0.0	13.039187	10
CGAACCT	290	0.0	12.449739	16
AGAACCG	185	0.0	12.3228245	5
AGTGACG	220	0.0	12.09233	19
CGACCAC	55	0.0030660955	12.091847	14
CGACCAT	200	0.0	11.874975	10
GCGTTAT	120	1.0004442E-8	11.874974	1
CGTCGTA	145	9.640644E-11	11.793079	10
GTCTTAA	105	2.718134E-7	11.76188	1
ACGGTAT	275	0.0	11.745898	9
TCCCGAC	220	0.0	11.65535	2
ACCTCCG	310	0.0	11.64653	19
TCTTCGA	310	0.0	11.645137	12
>>END_MODULE