FastQCFastQC Report
Thu 2 Feb 2017
SRR4064045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255285
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12970.10332314972297128No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12920.10292483380268226No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12730.10141123330558398No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12660.10085359101717938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT250.00235345435.198096
GTATCAA21800.021.4968151
AATACGG808.99267E-519.2489555
GTCTTAC1404.0781742E-918.8583721
TTAATAC1254.8556103E-717.5990433
GGCGTTA2650.017.43509942
TAGGACG2300.017.2164574
CGCAATA4000.016.50107636
ATACGAA4000.016.50107640
CGCGAGC800.001988070616.5004239
CCGTATG800.001989129716.49910414
TTAACCG800.001989129716.4991045
CGTACAC800.001989129716.4991043
TACCGTA800.001989129716.49910412
CTAGCGG4150.016.4341529
CGAATGC4300.016.37316143
GTAGGAC4050.016.295413
CCGATAA2301.8189894E-1216.2599879
GGTATCA10700.016.244051
GTCCTAA1906.166374E-1016.2115841
Produced by FastQC (version 0.11.5)