##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1255285
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305697909239733	32.0	32.0	32.0	32.0	32.0
2	31.35265776297813	32.0	32.0	32.0	32.0	32.0
3	31.446382295653976	32.0	32.0	32.0	32.0	32.0
4	31.530891391198015	32.0	32.0	32.0	32.0	32.0
5	31.476746714889448	32.0	32.0	32.0	32.0	32.0
6	35.04780747001677	36.0	36.0	36.0	36.0	36.0
7	35.06196202456016	36.0	36.0	36.0	36.0	36.0
8	35.012162178310106	36.0	36.0	36.0	36.0	36.0
9	35.130655588173205	36.0	36.0	36.0	36.0	36.0
10	34.967782615103346	36.0	36.0	36.0	36.0	36.0
11	35.12726193653234	36.0	36.0	36.0	36.0	36.0
12	35.022484137068474	36.0	36.0	36.0	36.0	36.0
13	35.07746448017781	36.0	36.0	36.0	36.0	36.0
14	35.016859119642156	36.0	36.0	36.0	36.0	36.0
15	34.976252404832366	36.0	36.0	36.0	36.0	36.0
16	35.00147376890507	36.0	36.0	36.0	36.0	36.0
17	34.95034912390413	36.0	36.0	36.0	36.0	36.0
18	34.959287333155416	36.0	36.0	36.0	36.0	36.0
19	34.94995319787937	36.0	36.0	36.0	36.0	36.0
20	34.9421971902795	36.0	36.0	36.0	36.0	36.0
21	34.94520367884584	36.0	36.0	36.0	36.0	36.0
22	34.926055039293864	36.0	36.0	36.0	32.0	36.0
23	34.871341567851125	36.0	36.0	36.0	32.0	36.0
24	34.84300537328176	36.0	36.0	36.0	32.0	36.0
25	34.81445727464281	36.0	36.0	36.0	32.0	36.0
26	34.76161190486623	36.0	36.0	36.0	32.0	36.0
27	34.75019776385442	36.0	36.0	36.0	32.0	36.0
28	34.72341181484683	36.0	36.0	36.0	32.0	36.0
29	34.702183169559106	36.0	36.0	36.0	32.0	36.0
30	34.68277243813158	36.0	36.0	36.0	32.0	36.0
31	34.67603611928765	36.0	36.0	36.0	32.0	36.0
32	34.62658679104745	36.0	36.0	36.0	32.0	36.0
33	34.61198054625045	36.0	36.0	36.0	32.0	36.0
34	34.59286536523578	36.0	36.0	36.0	32.0	36.0
35	34.556454510330326	36.0	36.0	36.0	32.0	36.0
36	34.53074082778014	36.0	36.0	36.0	32.0	36.0
37	34.51842649278849	36.0	36.0	36.0	32.0	36.0
38	34.471809190741546	36.0	36.0	36.0	32.0	36.0
39	34.473845381726065	36.0	36.0	36.0	32.0	36.0
40	34.45240483236874	36.0	36.0	36.0	32.0	36.0
41	34.42797532034558	36.0	36.0	36.0	32.0	36.0
42	34.36236551858741	36.0	36.0	36.0	32.0	36.0
43	34.35600520997224	36.0	36.0	36.0	32.0	36.0
44	34.28884436602046	36.0	36.0	36.0	32.0	36.0
45	34.26363017163433	36.0	36.0	36.0	32.0	36.0
46	34.23521351724907	36.0	36.0	36.0	32.0	36.0
47	34.20509525725234	36.0	36.0	36.0	32.0	36.0
48	34.16464786881067	36.0	36.0	36.0	32.0	36.0
49	34.149225873008916	36.0	36.0	36.0	32.0	36.0
50	33.52961837351677	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	10.0
21	39.0
22	114.0
23	360.0
24	1024.0
25	2336.0
26	4869.0
27	9024.0
28	14856.0
29	23011.0
30	33214.0
31	47402.0
32	69242.0
33	110500.0
34	226578.0
35	712700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.454252706166166	18.07807429797425	11.938042796742163	26.52963019911742
2	16.039672581705204	20.417916392822292	36.908725179741495	26.633685845731016
3	17.933927091963543	24.288126951755785	28.87284111911287	28.905104837167805
4	12.247178927494554	15.83042894641456	36.03572097173152	35.88667115435937
5	14.22545477720199	37.09986178437566	33.29698036700829	15.37770307141406
6	34.47586362926637	35.77302028863588	16.4315804232349	13.319535658862854
7	30.31909088374353	30.498572037425763	20.736565799798452	18.445771279032254
8	28.218532046507367	32.97330885018143	19.327563063368082	19.480596039943123
9	27.60254351248176	13.880258621239062	18.336663288912682	40.1805345773665
10	15.66952951420284	26.747482826499645	31.589282684220294	25.993704975077215
11	37.29057544701004	20.83718040126346	22.612633784359726	19.259610367366772
12	24.671945701357465	23.55936524122108	28.864078133962146	22.90461092345931
13	29.569250452290607	19.308009307905746	25.279041996308383	25.84369824349526
14	23.586534999251167	19.523868702221968	25.234369274204084	31.655227024322784
15	25.169569976594	27.36334420514422	22.13858133224562	25.32850448601615
16	25.820775670051844	25.727409748931706	23.63695314815228	24.814861432864166
17	24.121932469518875	25.856359312825372	25.22909140155423	24.792616816101525
18	24.878393858909792	24.75045447955121	26.245417364385055	24.125734297153947
19	25.79424257777523	24.643427304100108	25.494310450862116	24.068019667262547
20	26.026543878656554	24.01727104709706	25.01609202727678	24.9400930469696
21	27.00167691002442	23.959738226777187	24.40864026894291	24.629944594255488
22	25.98368178037799	24.130475653318292	24.904244776480862	24.98159778982286
23	24.47296409153373	24.04548804046922	25.49345760889048	25.98809025910657
24	24.918743487529515	24.760054298469512	25.25619700402459	25.065005209976388
25	24.89113826436234	24.354198565717756	25.44711781285501	25.307545357064896
26	24.698399243478626	25.160310670886915	25.657753746557372	24.483536339077087
27	25.38797979637036	24.658227243033092	24.847676104587244	25.106116856009304
28	24.681253126921963	24.4755534452705	25.73492624447511	25.108267183332426
29	24.73991257525387	24.68183654105832	25.486775154929177	25.091475728758635
30	24.704539280442113	24.801412953507807	25.61814724320311	24.87590052284697
31	25.267588331037537	24.541086510202017	24.775074430784745	25.416250727975697
32	24.900496799008316	24.74028788211938	24.738375891067495	25.62083942780481
33	24.710112194846033	24.31456620993653	25.260769565982887	25.714552029234554
34	25.296057270003438	24.518600721610966	25.415635329142393	24.769706679243207
35	25.787796925052643	24.448930524098557	25.231967407055695	24.5313051437931
36	24.864820939031397	24.947597449623043	24.974844385736947	25.212737225608617
37	25.62197318598291	24.743335731283047	24.838616390982114	24.79607469175193
38	24.854862263551166	24.68182031840674	25.321565631256536	25.141751786785555
39	25.451812148120297	24.50106475104424	24.67218972246366	25.37493337837181
40	25.64530918517492	24.742222870167	25.162696544356468	24.44977140030161
41	24.753834289847777	25.05831498376422	25.450905475553913	24.736945250834093
42	25.883007131612995	25.120373852026994	24.807051310648983	24.189567705711028
43	25.110594001116894	24.375451597150246	25.20730649335958	25.306647908373282
44	24.86275871903358	24.820775752805567	24.82300634683666	25.49345918132419
45	25.060604732865173	25.124017637709965	24.732227856269393	25.083149773155466
46	24.7912796311226	24.822747014980067	24.900260342811258	25.48571301108608
47	25.118660486814704	24.706077297010502	25.225490774037613	24.949771442137177
48	25.798020016522432	25.202049291662387	24.290367835266395	24.709562856548786
49	24.93750388360188	25.21887581874294	24.606899826173965	25.236720471481217
50	25.008524048971058	25.442613234422502	24.486087477455083	25.062775239151353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	23.5
2	25.0
3	73.0
4	121.0
5	142.5
6	164.0
7	182.5
8	201.0
9	267.5
10	334.0
11	507.5
12	681.0
13	1233.5
14	1786.0
15	2387.5
16	2989.0
17	3237.0
18	3485.0
19	3476.5
20	3468.0
21	3870.0
22	4272.0
23	5004.5
24	5737.0
25	6719.5
26	7702.0
27	9402.0
28	11102.0
29	12718.5
30	14335.0
31	16711.0
32	19087.0
33	22346.5
34	25606.0
35	30040.5
36	34475.0
37	40708.0
38	46941.0
39	50853.0
40	54765.0
41	60035.0
42	65305.0
43	66891.0
44	68477.0
45	74818.5
46	81160.0
47	86300.0
48	91440.0
49	94288.5
50	97137.0
51	94489.5
52	91842.0
53	90637.5
54	89433.0
55	89941.0
56	90449.0
57	88210.0
58	85971.0
59	78219.5
60	70468.0
61	62128.5
62	53789.0
63	46778.0
64	39767.0
65	33655.0
66	27543.0
67	24056.0
68	20569.0
69	18539.5
70	16510.0
71	13055.0
72	9600.0
73	8257.0
74	6914.0
75	5477.5
76	4041.0
77	3529.0
78	3017.0
79	2491.0
80	1965.0
81	1549.0
82	1133.0
83	939.5
84	746.0
85	543.5
86	341.0
87	237.5
88	134.0
89	109.5
90	85.0
91	72.0
92	59.0
93	45.5
94	32.0
95	30.0
96	28.0
97	24.0
98	20.0
99	16.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007647665669549146
2	7.96631840578036E-4
3	3.98315920289018E-4
4	0.0
5	0.0
6	7.966318405780361E-5
7	0.0
8	0.0
9	0.0010356213927514468
10	6.373054724624289E-4
11	0.0
12	3.98315920289018E-4
13	6.373054724624289E-4
14	7.966318405780361E-5
15	0.004062822386947984
16	7.169686565202324E-4
17	0.0
18	2.389895521734108E-4
19	7.966318405780361E-5
20	3.98315920289018E-4
21	0.0
22	7.966318405780361E-5
23	7.96631840578036E-4
24	7.966318405780361E-5
25	0.0018322532333294832
26	0.005257770147815038
27	0.005178106963757235
28	0.004540801491294806
29	0.0027085482579653226
30	0.003345853730427752
31	0.007329012933317933
32	0.003903496018832377
33	0.004620464675352609
34	0.003027200994196537
35	0.0035051800985433585
36	0.007806992037664754
37	0.0036645064666589663
38	0.007806992037664754
39	0.004939117411583824
40	0.0027085482579653226
41	0.002628885073907519
42	0.0023102323376763046
43	0.0014339373130404648
44	0.0011152845768092506
45	0.001593263681156072
46	0.0013542741289826613
47	0.0018322532333294832
48	0.0017525900492716792
49	5.576422884046253E-4
50	0.0010356213927514468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1255285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84469535534237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3491215295817	46.47770456948034
2	11.875222167226184	13.738372170804144
3	3.373340655399768	5.853895876241717
4	1.3959896191170738	3.2300237694819023
5	0.7583067885665203	2.193201258525919
6	0.46481540098583335	1.6132263159898104
7	0.3245719018700837	1.314233394920535
8	0.225831265227337	1.0450512591029466
9	0.16132629661667042	0.8398683432537212
>10	0.9285198750917992	9.67554852370227
>50	0.0692979092821577	2.797344411576897
>100	0.06602738741531548	7.81494589403115
>500	0.006242075688749486	2.4561651908031368
>1k	0.001387127930833219	0.950419022085628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1297	0.10332314972297128	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1292	0.10292483380268226	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1273	0.10141123330558398	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1266	0.10085359101717938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.966318405780361E-5	0.0
4	0.0	0.0	0.0	7.966318405780361E-5	0.0
5	0.0	0.0	0.0	7.966318405780361E-5	0.0
6	0.0	0.0	0.0	7.966318405780361E-5	0.0
7	0.0	0.0	0.0	7.966318405780361E-5	0.0
8	0.0	0.0	0.0	7.966318405780361E-5	0.0
9	0.0	0.0	0.0	7.966318405780361E-5	0.0
10	0.0	0.0	0.0	7.966318405780361E-5	0.0
11	0.0	0.0	0.0	7.966318405780361E-5	7.966318405780361E-5
12	0.0	0.0	0.0	7.966318405780361E-5	1.5932636811560722E-4
13	0.0	0.0	0.0	7.966318405780361E-5	2.389895521734108E-4
14	0.0	0.0	0.0	7.966318405780361E-5	2.389895521734108E-4
15	0.0	0.0	0.0	7.966318405780361E-5	6.373054724624289E-4
16	0.0	0.0	0.0	7.966318405780361E-5	6.373054724624289E-4
17	7.966318405780361E-5	0.0	0.0	7.966318405780361E-5	6.373054724624289E-4
18	7.966318405780361E-5	0.0	0.0	2.389895521734108E-4	6.373054724624289E-4
19	7.966318405780361E-5	0.0	0.0	2.389895521734108E-4	6.373054724624289E-4
20	7.966318405780361E-5	7.966318405780361E-5	0.0	2.389895521734108E-4	6.373054724624289E-4
21	7.966318405780361E-5	7.966318405780361E-5	0.0	4.779791043468216E-4	6.373054724624289E-4
22	7.966318405780361E-5	7.966318405780361E-5	0.0	6.373054724624289E-4	7.169686565202325E-4
23	7.966318405780361E-5	7.966318405780361E-5	0.0	0.0010356213927514468	8.762950246358396E-4
24	7.966318405780361E-5	7.966318405780361E-5	0.0	0.0020712427855028937	0.0010356213927514468
25	7.966318405780361E-5	7.966318405780361E-5	0.0	0.0023102323376763046	0.0010356213927514468
26	7.966318405780361E-5	7.966318405780361E-5	0.0	0.002628885073907519	0.0010356213927514468
27	7.966318405780361E-5	7.966318405780361E-5	0.0	0.0031068641782543405	0.0010356213927514468
28	7.966318405780361E-5	7.966318405780361E-5	0.0	0.005656086068104056	0.0010356213927514468
29	7.966318405780361E-5	7.966318405780361E-5	0.0	0.009559582086936433	0.0010356213927514468
30	7.966318405780361E-5	7.966318405780361E-5	0.0	0.015534320891271703	0.0010356213927514468
31	7.966318405780361E-5	7.966318405780361E-5	0.0	0.028121103972404672	0.0010356213927514468
32	7.966318405780361E-5	7.966318405780361E-5	0.0	0.04453171988831221	0.001194947760867054
33	7.966318405780361E-5	7.966318405780361E-5	0.0	0.05990671441146831	0.001194947760867054
34	7.966318405780361E-5	7.966318405780361E-5	0.0	0.07671564624766487	0.001433937313040465
35	7.966318405780361E-5	7.966318405780361E-5	0.0	0.0970297581824048	0.001433937313040465
36	7.966318405780361E-5	7.966318405780361E-5	0.0	0.12833738951712162	0.001433937313040465
37	7.966318405780361E-5	7.966318405780361E-5	0.0	0.17302843577354943	0.001433937313040465
38	7.966318405780361E-5	7.966318405780361E-5	0.0	0.23046559147922582	0.0015932636811560722
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGT	25	0.002353454	35.19809	6
GTATCAA	2180	0.0	21.496815	1
AATACGG	80	8.99267E-5	19.248955	5
GTCTTAC	140	4.0781742E-9	18.858372	1
TTAATAC	125	4.8556103E-7	17.599043	3
GGCGTTA	265	0.0	17.435099	42
TAGGACG	230	0.0	17.216457	4
CGCAATA	400	0.0	16.501076	36
ATACGAA	400	0.0	16.501076	40
CGCGAGC	80	0.0019880706	16.50042	39
CCGTATG	80	0.0019891297	16.499104	14
TTAACCG	80	0.0019891297	16.499104	5
CGTACAC	80	0.0019891297	16.499104	3
TACCGTA	80	0.0019891297	16.499104	12
CTAGCGG	415	0.0	16.43415	29
CGAATGC	430	0.0	16.373161	43
GTAGGAC	405	0.0	16.29541	3
CCGATAA	230	1.8189894E-12	16.259987	9
GGTATCA	1070	0.0	16.24405	1
GTCCTAA	190	6.166374E-10	16.211584	1
>>END_MODULE