FastQCFastQC Report
Thu 2 Feb 2017
SRR4064044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833786
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35560.19391575680041184No Hit
TATCAACGCAGAGTACTTTTTTTTT24170.13180382007497057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA508.7189284E-515.1992586
TACGCGT508.733148E-515.1963555
CAAGACG3800.013.9958774
GCGTTAT1400.013.5674321
ATACGCG500.001503057713.2960844
GTTATAC905.4260636E-712.6629381
CGTTATT1650.012.6598282
AAGACGG4100.012.5091945
CGAACGA1457.2759576E-1212.45004716
CGGACCA4000.012.3520949
CGCAAGA3850.012.3310022
GTATCAA22150.012.3056311
GTCCTAA3400.012.2904981
AATCGTA701.0904918E-412.21502213
CGCCACT1103.8060534E-812.09295917
CGCGTAT550.003066165112.09229857
GATCTAT550.00307558112.0873491
GACGGAC4150.011.9049837
GAAATCG802.8636458E-511.87636511
CGCCAGT2850.011.66864518
Produced by FastQC (version 0.11.5)