##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064044_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1833786
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23311607788477	32.0	32.0	32.0	32.0	32.0
2	30.83590996986562	32.0	32.0	32.0	32.0	32.0
3	30.85357887997836	32.0	32.0	32.0	32.0	32.0
4	30.875598897581288	32.0	32.0	32.0	32.0	32.0
5	30.78248225256382	32.0	32.0	32.0	32.0	32.0
6	34.43783462192426	36.0	36.0	36.0	32.0	36.0
7	34.3762538267824	36.0	36.0	36.0	32.0	36.0
8	34.33441415737714	36.0	36.0	36.0	32.0	36.0
9	34.47858147024789	36.0	36.0	36.0	32.0	36.0
10	34.17547576434764	36.0	36.0	36.0	32.0	36.0
11	34.44998816655815	36.0	36.0	36.0	32.0	36.0
12	34.267445601613275	36.0	36.0	36.0	32.0	36.0
13	34.349846710575825	36.0	36.0	36.0	32.0	36.0
14	34.24752615626905	36.0	36.0	36.0	32.0	36.0
15	34.18364029390561	36.0	36.0	36.0	32.0	36.0
16	34.18985148757816	36.0	36.0	36.0	32.0	36.0
17	34.11392496180034	36.0	36.0	36.0	32.0	36.0
18	34.10736803531056	36.0	36.0	36.0	32.0	36.0
19	34.13169421077487	36.0	36.0	36.0	32.0	36.0
20	34.1081456614894	36.0	36.0	36.0	32.0	36.0
21	34.08448913886353	36.0	36.0	36.0	32.0	36.0
22	34.06720304332131	36.0	36.0	36.0	32.0	36.0
23	34.00796930503341	36.0	36.0	36.0	32.0	36.0
24	34.00214692445029	36.0	36.0	36.0	32.0	36.0
25	33.600062929916575	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	18.0
6	80.0
7	34.0
8	107.0
9	110.0
10	168.0
11	67.0
12	121.0
13	97.0
14	168.0
15	285.0
16	521.0
17	656.0
18	854.0
19	1173.0
20	1723.0
21	2646.0
22	4191.0
23	6495.0
24	9746.0
25	14450.0
26	20450.0
27	27548.0
28	37695.0
29	50782.0
30	67233.0
31	90366.0
32	127433.0
33	178015.0
34	377417.0
35	813119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31262103720772	17.23624389972473	11.449151553040346	26.0019835100272
2	16.44776451494686	19.60303125490812	37.883160567509556	26.06604366263546
3	18.89889505594522	23.543728234587117	28.765191481100562	28.7921852283671
4	12.432814463213738	15.42049856903532	36.16975909186095	35.97692787588999
5	14.40990211755056	36.6926661242084	33.97406671364093	14.923365044600104
6	34.24099663164508	35.72016038954239	16.826994397710216	13.211848581102306
7	29.995592805800914	30.57518247856675	20.935754235651803	18.49347047998054
8	27.81274513975489	33.59692505419669	19.395811990150225	19.1945178158982
9	27.15935886761941	14.169379152613207	18.560605647281168	40.110656332486215
10	15.541568791145233	27.15911354106134	32.150642363526735	25.148675304266693
11	37.148480212314134	21.06859832951031	22.638365390701217	19.14455606747434
12	24.292730040761537	23.67776013445305	29.49487343188131	22.53463639290411
13	29.449153698483325	19.566650405279603	25.376276491600287	25.607919404636785
14	23.216276522772628	19.76001471190906	25.570291111354855	31.453417653963456
15	24.917606282439117	27.627977632561123	22.342775325973555	25.111640759026205
16	25.35699240176979	25.909288350762772	24.266206538276002	24.467512709191436
17	23.74060834514253	26.08556862312842	25.538862311750833	24.634960719978217
18	24.3514066635741	25.38390591317161	26.5207520397304	23.74393538352389
19	25.343971360277667	25.0553366586263	25.4291046812412	24.17158729985484
20	25.410021262383047	24.930526145745556	25.146206170893677	24.51324642097772
21	26.359629638927423	24.47529816511258	24.670727700785974	24.49434449517402
22	25.459544477662117	24.67258035820511	25.243211206543698	24.624663957589075
23	24.44525218389826	24.45987881725113	25.571011759158292	25.523857239692315
24	24.655304899080086	25.13119807197775	25.404236322437274	24.809260706504894
25	24.691149833466223	24.830423699260134	25.456992372136277	25.02143409513737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	106.0
2	310.0
3	514.0
4	514.0
5	514.0
6	1392.5
7	2271.0
8	2271.0
9	2271.0
10	2696.0
11	3121.0
12	3121.0
13	3121.0
14	4101.5
15	5082.0
16	5082.0
17	5082.0
18	8708.0
19	12334.0
20	12334.0
21	12334.0
22	19908.0
23	27482.0
24	27482.0
25	27482.0
26	41033.0
27	54584.0
28	54584.0
29	54584.0
30	70442.0
31	86300.0
32	86300.0
33	86300.0
34	103219.0
35	120138.0
36	120138.0
37	120138.0
38	137819.5
39	155501.0
40	155501.0
41	155501.0
42	176089.5
43	196678.0
44	196678.0
45	196678.0
46	216876.5
47	237075.0
48	237075.0
49	237075.0
50	241940.5
51	246806.0
52	246806.0
53	246806.0
54	230331.0
55	213856.0
56	213856.0
57	213856.0
58	194933.0
59	176010.0
60	176010.0
61	176010.0
62	153028.0
63	130046.0
64	130046.0
65	130046.0
66	105927.0
67	81808.0
68	81808.0
69	81808.0
70	61464.5
71	41121.0
72	41121.0
73	41121.0
74	31095.5
75	21070.0
76	21070.0
77	21070.0
78	16842.5
79	12615.0
80	12615.0
81	12615.0
82	8579.0
83	4543.0
84	4543.0
85	4543.0
86	3432.5
87	2322.0
88	2322.0
89	2322.0
90	1698.5
91	1075.0
92	1075.0
93	1075.0
94	734.5
95	394.0
96	394.0
97	394.0
98	664.0
99	934.0
100	934.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03664549734810932
2	0.004907879109121784
3	0.0015268957228378884
4	0.0031628554258784835
5	0.008616054436013798
6	0.013632997525338288
7	0.022903435842568327
8	0.035336729585676845
9	0.049296919051623256
10	0.060039721101589824
11	0.056167949804393755
12	0.06412962035919131
13	0.06674715588405628
14	0.06854671155740091
15	0.06118489289371824
16	0.06838311558709685
17	0.06407508836908996
18	0.07830793778554312
19	0.07449069847844841
20	0.07863512972615125
21	0.07699917002311066
22	0.0778716818647323
23	0.08245236903324597
24	0.07863512972615125
25	0.07770808589442825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1833786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.997937790631035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41293876831658	43.90962867128753
2	12.870658690055627	14.414606893003597
3	3.872985243218053	6.506375601412698
4	1.6564390911873363	3.710286927291115
5	0.856732090740984	2.398761516027544
6	0.5035437462463982	1.6918446826300264
7	0.3465007948971001	1.3582330969936414
8	0.2481869018506859	1.111836375222733
9	0.17824210589324985	0.8983071321733147
>10	0.9129084946708188	9.05951595996725
>50	0.06897603329241357	2.678647626087848
>100	0.06189892661106229	7.417649140240112
>500	0.007344936043933178	2.774144817121598
>1k	0.002644176975815944	2.0701615605409245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3556	0.19391575680041184	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2417	0.13180382007497057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0906398020270631E-4
2	0.0	0.0	0.0	0.0	1.0906398020270631E-4
3	0.0	0.0	0.0	0.0	1.0906398020270631E-4
4	0.0	0.0	0.0	0.0	1.0906398020270631E-4
5	0.0	0.0	0.0	0.0	1.0906398020270631E-4
6	0.0	0.0	0.0	0.0	1.0906398020270631E-4
7	0.0	0.0	0.0	0.0	1.0906398020270631E-4
8	0.0	0.0	0.0	0.0	1.0906398020270631E-4
9	0.0	0.0	0.0	0.0	1.0906398020270631E-4
10	0.0	0.0	0.0	5.4531990101353155E-5	1.0906398020270631E-4
11	0.0	0.0	0.0	5.4531990101353155E-5	1.0906398020270631E-4
12	0.0	0.0	0.0	5.4531990101353155E-5	2.1812796040541262E-4
13	0.0	0.0	0.0	5.4531990101353155E-5	2.7265995050676576E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTA	50	8.7189284E-5	15.199258	6
TACGCGT	50	8.733148E-5	15.196355	5
CAAGACG	380	0.0	13.995877	4
GCGTTAT	140	0.0	13.567432	1
ATACGCG	50	0.0015030577	13.296084	4
GTTATAC	90	5.4260636E-7	12.662938	1
CGTTATT	165	0.0	12.659828	2
AAGACGG	410	0.0	12.509194	5
CGAACGA	145	7.2759576E-12	12.450047	16
CGGACCA	400	0.0	12.352094	9
CGCAAGA	385	0.0	12.331002	2
GTATCAA	2215	0.0	12.305631	1
GTCCTAA	340	0.0	12.290498	1
AATCGTA	70	1.0904918E-4	12.215022	13
CGCCACT	110	3.8060534E-8	12.092959	17
CGCGTAT	55	0.0030661651	12.0922985	7
GATCTAT	55	0.003075581	12.087349	1
GACGGAC	415	0.0	11.904983	7
GAAATCG	80	2.8636458E-5	11.876365	11
CGCCAGT	285	0.0	11.668645	18
>>END_MODULE