FastQCFastQC Report
Thu 2 Feb 2017
SRR4064044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833786
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26900.024.454821
TTAACGG755.471246E-520.53497335
TAGGACG4350.019.7235644
GTAGGAC7150.019.384053
GATATAC5700.018.9132771
CGCAATA4700.018.7243936
GGTATCA12250.018.6785341
CGTTATA600.00741593818.33229842
AGGACGT5100.017.6857595
AACGCAG37050.017.6351136
TCAACGC38150.017.0689664
CGAAAAC4450.016.80941623
ATCAACG39150.016.6329773
TGTAGGA8600.016.6274222
CAACGCA39200.016.6117615
ATACACT7750.016.4640374
TAGCGGC5100.016.3930230
TATCAAC40550.016.3842332
CGTGCAA1351.0865242E-616.295829
TACACCG953.3482374E-416.2100545