##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064044_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1833786 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.340786220420487 32.0 32.0 32.0 32.0 32.0 2 31.385089645138528 32.0 32.0 32.0 32.0 32.0 3 31.46220878553986 32.0 32.0 32.0 32.0 32.0 4 31.54762278695551 32.0 32.0 32.0 32.0 32.0 5 31.494181981976087 32.0 32.0 32.0 32.0 32.0 6 35.0797394025257 36.0 36.0 36.0 36.0 36.0 7 35.098674000128696 36.0 36.0 36.0 36.0 36.0 8 35.041755690140505 36.0 36.0 36.0 36.0 36.0 9 35.158740987225336 36.0 36.0 36.0 36.0 36.0 10 35.006630544676426 36.0 36.0 36.0 36.0 36.0 11 35.15463636433041 36.0 36.0 36.0 36.0 36.0 12 35.0542887774255 36.0 36.0 36.0 36.0 36.0 13 35.09990805906469 36.0 36.0 36.0 36.0 36.0 14 35.04357651328999 36.0 36.0 36.0 36.0 36.0 15 35.01368589355574 36.0 36.0 36.0 36.0 36.0 16 35.037095386266444 36.0 36.0 36.0 36.0 36.0 17 34.99746480778019 36.0 36.0 36.0 36.0 36.0 18 34.99413235786509 36.0 36.0 36.0 36.0 36.0 19 34.9898292385262 36.0 36.0 36.0 36.0 36.0 20 34.98441093998973 36.0 36.0 36.0 36.0 36.0 21 34.9830525481163 36.0 36.0 36.0 36.0 36.0 22 34.960431042662556 36.0 36.0 36.0 36.0 36.0 23 34.91477740586961 36.0 36.0 36.0 32.0 36.0 24 34.88706043126079 36.0 36.0 36.0 32.0 36.0 25 34.864159722017725 36.0 36.0 36.0 32.0 36.0 26 34.79986159780912 36.0 36.0 36.0 32.0 36.0 27 34.80041891474796 36.0 36.0 36.0 32.0 36.0 28 34.771382811298594 36.0 36.0 36.0 32.0 36.0 29 34.75069010233473 36.0 36.0 36.0 32.0 36.0 30 34.732587117580785 36.0 36.0 36.0 32.0 36.0 31 34.72028306465422 36.0 36.0 36.0 32.0 36.0 32 34.68862997099989 36.0 36.0 36.0 32.0 36.0 33 34.66342801177455 36.0 36.0 36.0 32.0 36.0 34 34.66486711099332 36.0 36.0 36.0 32.0 36.0 35 34.613669206766765 36.0 36.0 36.0 32.0 36.0 36 34.58803753545943 36.0 36.0 36.0 32.0 36.0 37 34.58403924994519 36.0 36.0 36.0 32.0 36.0 38 34.54066559565838 36.0 36.0 36.0 32.0 36.0 39 34.54930182693073 36.0 36.0 36.0 32.0 36.0 40 34.5212522071823 36.0 36.0 36.0 32.0 36.0 41 34.512593617794 36.0 36.0 36.0 32.0 36.0 42 34.45161976370198 36.0 36.0 36.0 32.0 36.0 43 34.43849718560399 36.0 36.0 36.0 32.0 36.0 44 34.37679478412421 36.0 36.0 36.0 32.0 36.0 45 34.352576582000296 36.0 36.0 36.0 32.0 36.0 46 34.33447032532695 36.0 36.0 36.0 32.0 36.0 47 34.303725734627704 36.0 36.0 36.0 32.0 36.0 48 34.256536476993496 36.0 36.0 36.0 32.0 36.0 49 34.24800167522274 36.0 36.0 36.0 32.0 36.0 50 33.65345411078501 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 1.0 19 4.0 20 16.0 21 56.0 22 176.0 23 499.0 24 1351.0 25 3203.0 26 6510.0 27 12208.0 28 20295.0 29 31628.0 30 45834.0 31 66533.0 32 96305.0 33 156423.0 34 323528.0 35 1069214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37553548007101 18.10911113716743 12.078303479154794 26.43704990360677 2 15.667855467940539 20.45670691140704 37.423791294484616 26.451646326167804 3 18.072763521826 24.231585537164847 29.08746760782124 28.608183333187913 4 12.17257629843395 16.162409354199454 36.30003719081725 35.36497715654935 5 14.116914405497697 37.11479965492156 33.669141328377464 15.099144611203268 6 34.28568312447513 36.09353357545616 16.537861684607748 13.08292161546096 7 29.877019321795807 30.832857086767035 20.892809622071084 18.397313969366074 8 27.765889803935686 33.80143593636335 19.242212559153575 19.19046170054739 9 27.32356147366435 13.953665919753252 18.360109697568234 40.36266290901417 10 15.417842526168837 27.35268695825256 32.14621284624802 25.08325766933058 11 37.374371927804006 20.962805910831474 22.634538599378555 19.02828356198597 12 24.287730411291175 23.684606600770213 29.423934962967323 22.60372802497129 13 29.495815183130723 19.62261393455675 25.32070508220215 25.56086580011037 14 23.25737382374369 19.696867242815948 25.45032566540007 31.595433268040292 15 24.90362387324869 27.774648102389648 22.25842708271414 25.06330094164752 16 25.506934355927246 25.9358872005619 24.08025442695041 24.476924016560435 17 23.858945373124236 26.16232210301529 25.404381972596585 24.574350551263887 18 24.517636229388227 25.426850083597724 26.34037197442226 23.71514171259179 19 25.386891047750964 25.100270751478497 25.40668617095243 24.10615202981811 20 25.655929775310003 24.776212515085035 25.06283499957192 24.505022710033042 21 26.454449973988243 24.52330860852902 24.5950181755123 24.427223241970434 22 25.54619300855499 24.700782643975515 25.199587301448805 24.553437046020687 23 24.527286350444495 24.391828000615128 25.507178092829324 25.57370755611106 24 24.77005185455422 25.111204542740335 25.37764828226677 24.741095320438678 25 24.846053001830132 24.736168764737577 25.48021865599853 24.93755957743376 26 24.3985478437705 25.592753834214893 25.918218321883725 24.090480000130885 27 25.06373791297396 25.07120922341454 25.13043435581216 24.73461850779934 28 24.446055021756425 25.157999270332134 25.687362948687113 24.708582759224328 29 24.523839387477985 24.975541655532712 25.51886046473581 24.981758492253494 30 24.410445461808404 25.323182719233905 25.691887779725857 24.574484039231834 31 25.055736131410743 24.907342771754543 24.994655439453766 25.042265657380945 32 24.76958104404968 25.205142537730573 24.92909242116457 25.096183997055178 33 24.49562002675484 24.73072012835342 25.504352705778032 25.26930713911371 34 25.05989142378806 24.990088535148207 25.560127281204753 24.389892759858977 35 25.36031887040736 24.936590750075123 25.513831696499416 24.1892586830181 36 24.45619511641782 25.399993673833777 25.28208702405143 24.861724185696975 37 25.247815509758016 25.14894561501383 25.01550125073416 24.587737624493993 38 24.679966645524715 25.06739338265971 25.3774329379261 24.875207033889478 39 25.29678691928934 24.75897395626275 24.955241331433374 24.98899779301454 40 25.541813224267212 25.02336741649625 25.324117246080434 24.1107021131561 41 24.575235967899204 25.194576719288182 25.885574928397077 24.34461238441554 42 25.50931263108413 25.52774484579653 25.07621011852241 23.886732404596927 43 24.761936442947093 24.842154400208532 25.48367992653322 24.91222923031115 44 24.614877042587246 25.23284063116048 25.05899065583649 25.093291670415784 45 24.62729623582559 25.430672028322103 25.175458974501595 24.766572761350712 46 24.66072330312572 25.013442370132527 25.17638679997426 25.149447526767492 47 24.936250643492222 24.940885968815714 25.500178869024246 24.622684518667818 48 25.368057059000922 25.331956192525595 24.66681681427849 24.633169934194992 49 24.74495249147115 25.43822146216332 24.873648689916326 24.943177356449205 50 24.717357920958378 25.78859484951204 24.719648287024313 24.774398942505268 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 23.5 2 29.0 3 87.5 4 146.0 5 186.5 6 227.0 7 227.0 8 227.0 9 289.0 10 351.0 11 569.0 12 787.0 13 1464.0 14 2141.0 15 2893.0 16 3645.0 17 3883.0 18 4121.0 19 4201.5 20 4282.0 21 5103.5 22 5925.0 23 7067.5 24 8210.0 25 10218.5 26 12227.0 27 15891.5 28 19556.0 29 22898.0 30 26240.0 31 29422.5 32 32605.0 33 37619.0 34 42633.0 35 49418.0 36 56203.0 37 64321.5 38 72440.0 39 77489.5 40 82539.0 41 89231.5 42 95924.0 43 98712.5 44 101501.0 45 110298.5 46 119096.0 47 126707.0 48 134318.0 49 139109.5 50 143901.0 51 140191.0 52 136481.0 53 134832.5 54 133184.0 55 132173.5 56 131163.0 57 125890.5 58 120618.0 59 110186.0 60 99754.0 61 86842.5 62 73931.0 63 63690.0 64 53449.0 65 45028.5 66 36608.0 67 31485.0 68 26362.0 69 23614.5 70 20867.0 71 16078.5 72 11290.0 73 9662.0 74 8034.0 75 6239.0 76 4444.0 77 3840.5 78 3237.0 79 2721.5 80 2206.0 81 1792.0 82 1378.0 83 1077.0 84 776.0 85 549.5 86 323.0 87 245.5 88 168.0 89 125.5 90 83.0 91 64.0 92 45.0 93 37.5 94 30.0 95 31.5 96 33.0 97 25.0 98 17.0 99 15.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008234330505304328 2 3.271919406081189E-4 3 5.453199010135315E-4 4 0.0 5 0.0 6 3.271919406081189E-4 7 1.0906398020270631E-4 8 0.0 9 9.270438317230036E-4 10 0.0011451717921284162 11 0.0 12 0.0 13 7.634478614189442E-4 14 1.0906398020270631E-4 15 0.004635219158615018 16 9.815758218243568E-4 17 0.0 18 2.1812796040541262E-4 19 5.4531990101353155E-5 20 8.179798515202975E-4 21 0.0 22 1.0906398020270631E-4 23 6.543838812162378E-4 24 1.6359597030405945E-4 25 0.0020722156238514203 26 0.0053986670200339626 27 0.005725858960642082 28 0.004089899257601487 29 0.003053791445675777 30 0.003871771297196074 31 0.007961670554797561 32 0.0033264513961825423 33 0.0053986670200339626 34 0.002781131495169011 35 0.0034355153763852486 36 0.0071982226933786165 37 0.0034355153763852486 38 0.008125266525101621 39 0.0034355153763852486 40 0.0019631516436487135 41 0.0033809833862838952 42 0.002181279604054126 43 0.001745023683243301 44 0.0011451717921284162 45 0.0013087677624324756 46 0.001745023683243301 47 0.0022903435842568324 48 0.0019086196535473606 49 5.453199010135315E-4 50 9.815758218243568E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1833786.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.203742052665575 #Duplication Level Percentage of deduplicated Percentage of total 1 79.39077894578465 47.002311980664274 2 12.602824672405086 14.922687620800806 3 3.5814661886527723 6.3610860121002615 4 1.4841374344304277 3.514659593548956 5 0.7987193631999484 2.364358757567953 6 0.4952924150388065 1.759389862835957 7 0.32890024285455244 1.3630487597313992 8 0.229536529520632 1.0871537188322844 9 0.16589252730068105 0.8839312555296871 >10 0.8063879446707698 8.390399019221691 >50 0.05758963431537846 2.3682434554815464 >100 0.05077117315225391 6.273018955092727 >500 0.006125220391562926 2.4857047266425023 >1k 0.001577708282675299 1.2240062819499342 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4531990101353155E-5 2 0.0 0.0 0.0 0.0 5.4531990101353155E-5 3 0.0 0.0 0.0 0.0 5.4531990101353155E-5 4 0.0 0.0 0.0 0.0 5.4531990101353155E-5 5 0.0 0.0 0.0 0.0 5.4531990101353155E-5 6 0.0 0.0 0.0 0.0 1.6359597030405948E-4 7 0.0 0.0 0.0 0.0 1.6359597030405948E-4 8 0.0 0.0 0.0 0.0 1.6359597030405948E-4 9 0.0 0.0 0.0 0.0 1.6359597030405948E-4 10 0.0 0.0 0.0 0.0 1.6359597030405948E-4 11 0.0 0.0 0.0 0.0 1.6359597030405948E-4 12 5.4531990101353155E-5 0.0 0.0 0.0 2.7265995050676576E-4 13 5.4531990101353155E-5 0.0 0.0 0.0 3.2719194060811896E-4 14 5.4531990101353155E-5 0.0 0.0 0.0 3.817239307094721E-4 15 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 16 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 17 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 18 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 19 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 20 5.4531990101353155E-5 0.0 0.0 0.0 4.3625592081082524E-4 21 5.4531990101353155E-5 0.0 0.0 5.4531990101353155E-5 4.3625592081082524E-4 22 5.4531990101353155E-5 0.0 0.0 5.4531990101353155E-5 5.453199010135315E-4 23 5.4531990101353155E-5 0.0 0.0 1.6359597030405948E-4 5.453199010135315E-4 24 5.4531990101353155E-5 0.0 0.0 2.7265995050676576E-4 5.453199010135315E-4 25 5.4531990101353155E-5 0.0 0.0 3.817239307094721E-4 5.453199010135315E-4 26 5.4531990101353155E-5 0.0 0.0 4.907879109121784E-4 5.453199010135315E-4 27 5.4531990101353155E-5 0.0 0.0 7.089158713175911E-4 5.453199010135315E-4 28 5.4531990101353155E-5 0.0 0.0 0.0012542357723311227 5.453199010135315E-4 29 5.4531990101353155E-5 0.0 0.0 0.0028356634852703642 5.453199010135315E-4 30 5.4531990101353155E-5 0.0 0.0 0.006761966772567791 5.453199010135315E-4 31 5.4531990101353155E-5 0.0 0.0 0.01603240508979783 5.453199010135315E-4 32 1.0906398020270631E-4 0.0 0.0 0.024593927535710273 5.453199010135315E-4 33 1.0906398020270631E-4 0.0 0.0 0.03402796182324437 5.453199010135315E-4 34 1.0906398020270631E-4 0.0 0.0 0.04564327571483259 5.453199010135315E-4 35 1.0906398020270631E-4 0.0 0.0 0.06031238105209659 5.453199010135315E-4 36 1.0906398020270631E-4 0.0 0.0 0.08168892117182702 5.453199010135315E-4 37 1.0906398020270631E-4 0.0 0.0 0.1141899872722335 5.453199010135315E-4 38 1.0906398020270631E-4 0.0 0.0 0.16048764686828235 5.453199010135315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2690 0.0 24.45482 1 TTAACGG 75 5.471246E-5 20.534973 35 TAGGACG 435 0.0 19.723564 4 GTAGGAC 715 0.0 19.38405 3 GATATAC 570 0.0 18.913277 1 CGCAATA 470 0.0 18.72439 36 GGTATCA 1225 0.0 18.678534 1 CGTTATA 60 0.007415938 18.332298 42 AGGACGT 510 0.0 17.685759 5 AACGCAG 3705 0.0 17.635113 6 TCAACGC 3815 0.0 17.068966 4 CGAAAAC 445 0.0 16.809416 23 ATCAACG 3915 0.0 16.632977 3 TGTAGGA 860 0.0 16.627422 2 CAACGCA 3920 0.0 16.611761 5 ATACACT 775 0.0 16.464037 4 TAGCGGC 510 0.0 16.39302 30 TATCAAC 4055 0.0 16.384233 2 CGTGCAA 135 1.0865242E-6 16.29582 9 TACACCG 95 3.3482374E-4 16.210054 5 >>END_MODULE