FastQCFastQC Report
Thu 2 Feb 2017
SRR4064043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349441
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22650.1678472789844091No Hit
TATCAACGCAGAGTACTTTTTTTTT14850.11004556701626822No Hit
GATTAAGAGGGACGGCCGGGGGCAT14700.10893399563226551No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13820.10241277684611629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1250.017.4797951
CCTATAC851.7220373E-814.5254733
CGTTATT1450.014.4104192
CGCATCG1800.012.66698713
GTTATTC1850.012.3209833
TAATATA2500.012.1580554
CCGCATC2050.012.04908612
ATCGCCA1900.012.00163816
GTCCTAA1900.011.999861
GTATCAA16450.011.95426751
CGAGCCG2800.011.87662215
GTATTAC802.8660459E-511.8748611
ACTAGAC907.4809595E-611.6079633
GACCGAG752.0729398E-411.4002897
CTATTAC752.0736537E-411.3998661
TATACTG1251.8326318E-811.3990225
TATTCCC2350.011.3181775
TCGCGTA855.3220516E-511.176349
CAGACCG855.3260723E-511.1755115
ATTCCCA2300.011.151636
Produced by FastQC (version 0.11.5)