##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064043_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1349441
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278341179792225	32.0	32.0	32.0	32.0	32.0
2	30.84929611594727	32.0	32.0	32.0	32.0	32.0
3	30.871213339449447	32.0	32.0	32.0	32.0	32.0
4	30.9026863716161	32.0	32.0	32.0	32.0	32.0
5	30.78412690884596	32.0	32.0	32.0	32.0	32.0
6	34.45346258191355	36.0	36.0	36.0	32.0	36.0
7	34.405364147080164	36.0	36.0	36.0	32.0	36.0
8	34.365694387527874	36.0	36.0	36.0	32.0	36.0
9	34.51789518771106	36.0	36.0	36.0	32.0	36.0
10	34.19381580965748	36.0	36.0	36.0	32.0	36.0
11	34.47246748838964	36.0	36.0	36.0	32.0	36.0
12	34.29188011924938	36.0	36.0	36.0	32.0	36.0
13	34.38217083962915	36.0	36.0	36.0	32.0	36.0
14	34.28227095515847	36.0	36.0	36.0	32.0	36.0
15	34.20763190091304	36.0	36.0	36.0	32.0	36.0
16	34.20382365735145	36.0	36.0	36.0	32.0	36.0
17	34.128809633025824	36.0	36.0	36.0	32.0	36.0
18	34.12461382157501	36.0	36.0	36.0	32.0	36.0
19	34.13680109022921	36.0	36.0	36.0	32.0	36.0
20	34.127302342229115	36.0	36.0	36.0	32.0	36.0
21	34.10870649402234	36.0	36.0	36.0	32.0	36.0
22	34.08934440260819	36.0	36.0	36.0	32.0	36.0
23	34.02798714430642	36.0	36.0	36.0	32.0	36.0
24	34.003497003574076	36.0	36.0	36.0	32.0	36.0
25	33.59843668600554	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	17.0
5	11.0
6	39.0
7	14.0
8	66.0
9	83.0
10	97.0
11	44.0
12	86.0
13	82.0
14	113.0
15	185.0
16	339.0
17	470.0
18	573.0
19	869.0
20	1281.0
21	1910.0
22	2860.0
23	4483.0
24	6871.0
25	10029.0
26	14380.0
27	19338.0
28	26627.0
29	36774.0
30	48829.0
31	67211.0
32	95076.0
33	134971.0
34	285346.0
35	590365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42992020448528	16.96603575527003	11.237275269320982	26.36676877092371
2	17.04826478153863	19.53290760347985	36.947121209987785	26.47170640499374
3	18.95024373470637	23.36096005833626	28.355261305244188	29.333534901713183
4	12.955447885186299	15.210992258701364	35.461758162601356	36.371801693510974
5	15.021977362316328	36.154261740713814	33.4786633198527	15.345097577117158
6	34.72980844910924	34.937545486481156	16.639640347379608	13.69300571702999
7	30.602290153850486	30.00992464239045	20.76103254823161	18.626752655527447
8	28.33989730406492	32.65225187560553	19.25774870999999	19.75010211032956
9	27.63736948082951	13.959953856784033	18.193504956837113	40.209171705549345
10	16.160732424525072	26.218075428218928	31.409493106828222	26.21169904042778
11	37.70973972292966	20.752471723923176	22.19335609506545	19.344432458081712
12	24.904290130482785	23.275053444035215	28.503337147145164	23.317319278336832
13	29.735162783386055	19.079683998689013	24.918990183908967	26.266163034015968
14	23.94455077271942	19.309569025671294	24.64218949404137	32.103690707567914
15	25.36880614765889	26.82438183818797	21.833783651521937	25.9730283626312
16	26.063966257991705	25.344323610062684	23.31961031776987	25.272099814175736
17	24.560403137424718	25.527019431686565	24.737472768499995	25.17510466238872
18	25.276746336684617	24.587578062601366	25.5348229097837	24.600852690930314
19	26.016267397572353	24.67266460209542	24.662134102208142	24.64893389812409
20	26.050643275200752	24.143511033719665	24.331509953930993	25.474335737148586
21	27.18623861579317	23.860837735239002	23.94864101436518	25.00428263460265
22	26.376730554162968	23.900632744294768	24.347306312907328	25.375330388634925
23	24.847109883606233	23.91145055378632	24.96250984533735	26.278929717270096
24	25.25616733472507	24.610974945028865	24.618761749732094	25.514095970513967
25	25.242765247081405	24.10773481892167	24.81656336072832	25.832936573268604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	73.0
2	193.0
3	313.0
4	313.0
5	313.0
6	829.5
7	1346.0
8	1346.0
9	1346.0
10	1648.0
11	1950.0
12	1950.0
13	1950.0
14	2480.0
15	3010.0
16	3010.0
17	3010.0
18	5163.5
19	7317.0
20	7317.0
21	7317.0
22	11881.0
23	16445.0
24	16445.0
25	16445.0
26	25113.5
27	33782.0
28	33782.0
29	33782.0
30	42669.0
31	51556.0
32	51556.0
33	51556.0
34	65608.5
35	79661.0
36	79661.0
37	79661.0
38	92658.5
39	105656.0
40	105656.0
41	105656.0
42	123007.5
43	140359.0
44	140359.0
45	140359.0
46	156928.0
47	173497.0
48	173497.0
49	173497.0
50	179048.0
51	184599.0
52	184599.0
53	184599.0
54	173405.5
55	162212.0
56	162212.0
57	162212.0
58	150100.0
59	137988.0
60	137988.0
61	137988.0
62	121797.0
63	105606.0
64	105606.0
65	105606.0
66	87320.0
67	69034.0
68	69034.0
69	69034.0
70	52191.5
71	35349.0
72	35349.0
73	35349.0
74	27218.5
75	19088.0
76	19088.0
77	19088.0
78	15404.5
79	11721.0
80	11721.0
81	11721.0
82	8033.5
83	4346.0
84	4346.0
85	4346.0
86	3344.0
87	2342.0
88	2342.0
89	2342.0
90	1682.5
91	1023.0
92	1023.0
93	1023.0
94	716.5
95	410.0
96	410.0
97	410.0
98	584.0
99	758.0
100	758.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03653364615422238
2	0.005335542643213004
3	0.0017044094554708208
4	0.004075761741343267
5	0.007262266375484367
6	0.01030056149175844
7	0.02030470394778282
8	0.03038295116274072
9	0.04424054108330783
10	0.05298490263746248
11	0.05083586462839057
12	0.061803368950550636
13	0.0621738927452182
14	0.06610144496869445
15	0.056690140584138175
16	0.06335956888815443
17	0.06069179756654793
18	0.07440117796924801
19	0.07240034947804314
20	0.07625379694258586
21	0.07202982568337556
22	0.07625379694258586
23	0.08055187296072966
24	0.07454938748711504
25	0.07551274935325071
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1349441.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.259159921495915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57855584812756	46.13860415551127
2	11.731174149837614	13.434343534249415
3	3.3184132487451707	5.700286646865315
4	1.4085013003075026	3.2259840481576894
5	0.7916315128900708	2.266407769773416
6	0.4784795363018924	1.6438401772963958
7	0.3230355328400586	1.294772026465019
8	0.23236530862812196	1.0644033893556313
9	0.1588752099698269	0.8187354949701205
>10	0.8333940354041033	8.611256249284057
>50	0.06445591823186737	2.5792744805002084
>100	0.07097862845854272	8.519051435258831
>500	0.007929820329655973	3.078045274414249
>1k	0.0022099499279369106	1.6249953178984686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2265	0.1678472789844091	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1485	0.11004556701626822	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1470	0.10893399563226551	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1382	0.10241277684611629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.410475893351395E-5
2	0.0	0.0	0.0	0.0	7.410475893351395E-5
3	0.0	0.0	0.0	0.0	7.410475893351395E-5
4	0.0	0.0	0.0	0.0	7.410475893351395E-5
5	0.0	0.0	0.0	0.0	7.410475893351395E-5
6	0.0	0.0	0.0	0.0	1.482095178670279E-4
7	0.0	0.0	0.0	0.0	1.482095178670279E-4
8	0.0	0.0	0.0	0.0	1.482095178670279E-4
9	0.0	0.0	0.0	0.0	2.964190357340558E-4
10	0.0	0.0	0.0	0.0	3.7052379466756975E-4
11	0.0	0.0	0.0	0.0	3.7052379466756975E-4
12	0.0	0.0	0.0	0.0	5.187333125345977E-4
13	0.0	0.0	0.0	0.0	5.928380714681116E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	125	0.0	17.479795	1
CCTATAC	85	1.7220373E-8	14.525473	3
CGTTATT	145	0.0	14.410419	2
CGCATCG	180	0.0	12.666987	13
GTTATTC	185	0.0	12.320983	3
TAATATA	250	0.0	12.158055	4
CCGCATC	205	0.0	12.049086	12
ATCGCCA	190	0.0	12.001638	16
GTCCTAA	190	0.0	11.99986	1
GTATCAA	1645	0.0	11.9542675	1
CGAGCCG	280	0.0	11.876622	15
GTATTAC	80	2.8660459E-5	11.874861	1
ACTAGAC	90	7.4809595E-6	11.607963	3
GACCGAG	75	2.0729398E-4	11.400289	7
CTATTAC	75	2.0736537E-4	11.399866	1
TATACTG	125	1.8326318E-8	11.399022	5
TATTCCC	235	0.0	11.318177	5
TCGCGTA	85	5.3220516E-5	11.17634	9
CAGACCG	85	5.3260723E-5	11.175511	5
ATTCCCA	230	0.0	11.15163	6
>>END_MODULE