FastQCFastQC Report
Thu 2 Feb 2017
SRR4064043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349441
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14080.10433950057838763No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13980.10359845298905251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.026.713881
TAGATCG450.001398421524.4440755
GGACCGT856.115102E-620.7055686
TAGGACG3000.019.0663764
GGTATCA7250.018.8135071
CGCAATA3450.018.49452836
GTATAGA1801.4551915E-1118.3330551
AGCGAAA5400.017.11021617
AACGCAG23950.017.0853356
GTATTAC1053.775357E-516.7616521
GTCTAGC1053.775357E-516.7616521
ATCAACG24400.016.5899123
ACGGACC6250.016.5437498
CAACGCA24650.016.5109065
GTCTAGT800.001988713216.499751
GTATAGT1206.408567E-616.499751
TCAACGC24650.016.4216584
TAAGACT2151.2732926E-1116.3718454
CAATACG3650.016.27493338
ATACGAA3950.016.15165540
Produced by FastQC (version 0.11.5)