##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1349441
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32927263955964	32.0	32.0	32.0	32.0	32.0
2	31.388741708603785	32.0	32.0	32.0	32.0	32.0
3	31.471791652988163	32.0	32.0	32.0	32.0	32.0
4	31.553391367240213	32.0	32.0	32.0	32.0	32.0
5	31.493341316886028	32.0	32.0	32.0	32.0	32.0
6	35.088519616641264	36.0	36.0	36.0	36.0	36.0
7	35.10591867299126	36.0	36.0	36.0	36.0	36.0
8	35.04945529296946	36.0	36.0	36.0	36.0	36.0
9	35.17287454583046	36.0	36.0	36.0	36.0	36.0
10	35.0133484902267	36.0	36.0	36.0	36.0	36.0
11	35.1626851414771	36.0	36.0	36.0	36.0	36.0
12	35.059723248367284	36.0	36.0	36.0	36.0	36.0
13	35.107047288469815	36.0	36.0	36.0	36.0	36.0
14	35.04896694260809	36.0	36.0	36.0	36.0	36.0
15	35.01425997876158	36.0	36.0	36.0	36.0	36.0
16	35.02978640785333	36.0	36.0	36.0	36.0	36.0
17	34.988438916558785	36.0	36.0	36.0	36.0	36.0
18	34.98877461111675	36.0	36.0	36.0	36.0	36.0
19	34.98984542488334	36.0	36.0	36.0	36.0	36.0
20	34.98702796194869	36.0	36.0	36.0	36.0	36.0
21	34.981627948165205	36.0	36.0	36.0	36.0	36.0
22	34.96070521052791	36.0	36.0	36.0	36.0	36.0
23	34.91282538473338	36.0	36.0	36.0	32.0	36.0
24	34.88052978974257	36.0	36.0	36.0	32.0	36.0
25	34.85135622824562	36.0	36.0	36.0	32.0	36.0
26	34.78961214310222	36.0	36.0	36.0	32.0	36.0
27	34.78644638780058	36.0	36.0	36.0	32.0	36.0
28	34.756600696140104	36.0	36.0	36.0	32.0	36.0
29	34.72337434537709	36.0	36.0	36.0	32.0	36.0
30	34.723008267867954	36.0	36.0	36.0	32.0	36.0
31	34.71280626570558	36.0	36.0	36.0	32.0	36.0
32	34.6709748703352	36.0	36.0	36.0	32.0	36.0
33	34.650404130302846	36.0	36.0	36.0	32.0	36.0
34	34.64798831516161	36.0	36.0	36.0	32.0	36.0
35	34.60040787259317	36.0	36.0	36.0	32.0	36.0
36	34.58192466361997	36.0	36.0	36.0	32.0	36.0
37	34.57482913295209	36.0	36.0	36.0	32.0	36.0
38	34.52596890119687	36.0	36.0	36.0	32.0	36.0
39	34.528981259647516	36.0	36.0	36.0	32.0	36.0
40	34.51242032812105	36.0	36.0	36.0	32.0	36.0
41	34.49721032634995	36.0	36.0	36.0	32.0	36.0
42	34.43659263354233	36.0	36.0	36.0	32.0	36.0
43	34.42589338844751	36.0	36.0	36.0	32.0	36.0
44	34.37159534948175	36.0	36.0	36.0	32.0	36.0
45	34.34252553464731	36.0	36.0	36.0	32.0	36.0
46	34.31945820528649	36.0	36.0	36.0	32.0	36.0
47	34.293880206693	36.0	36.0	36.0	32.0	36.0
48	34.26270952194279	36.0	36.0	36.0	32.0	36.0
49	34.2400638486603	36.0	36.0	36.0	32.0	36.0
50	33.62925833734116	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	7.0
21	37.0
22	134.0
23	398.0
24	1087.0
25	2435.0
26	4912.0
27	8995.0
28	14918.0
29	23297.0
30	34085.0
31	48589.0
32	70735.0
33	115399.0
34	241110.0
35	783298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60280439632108	17.931238929526945	11.738666429508415	26.72729024464356
2	16.269401638463503	20.29026963136424	36.518617050839794	26.92171167933246
3	18.214740929570787	24.048864714251838	28.734808513161013	29.001585843016365
4	12.51933207898678	15.939414913286315	35.57495288789951	35.966300119827395
5	14.590115462624894	36.52623567832903	33.31838887361507	15.565259985431004
6	34.673172076437574	35.4915850340993	16.364331601011084	13.470911288452033
7	30.410911192791083	30.30212532606118	20.795515176665877	18.491448304481857
8	28.346255968212024	32.947642764670704	19.091312625005465	19.614788642111808
9	27.604268175650592	13.915960009752274	18.042297890441066	40.43747392415607
10	16.043034370732006	26.53151360608493	31.357244116603045	26.06820790658002
11	37.93343905620109	20.642711050509842	22.105465971069478	19.31838392221959
12	24.941160777804125	23.370583353094617	28.486557386767846	23.201698482333413
13	29.850567089189177	19.07353818449944	24.99075539021887	26.08513933609251
14	23.871881384607065	19.216754839240366	24.67836586665402	32.23299790949856
15	25.38819823992589	26.985085687818433	21.730060213061602	25.896655859194073
16	26.11691768660499	25.400910608050225	23.32076998550497	25.161401719839816
17	24.583438623844987	25.624239963066188	24.63709046931285	25.15523094377598
18	25.379861275788475	24.655634930045057	25.578980910599952	24.385522883566516
19	26.10555037233936	24.637164574071782	24.7134924757733	24.543792577815555
20	26.12708615705434	24.07273880620795	24.361304875906956	25.438870160830753
21	27.13990459753335	23.981856190822718	23.93190958330153	24.946329628342404
22	26.33731498793202	24.023019936432842	24.40755010044915	25.232114975185986
23	24.952850427847633	23.750564127493927	25.084683966329465	26.21190147832898
24	25.352424751952114	24.472576341096325	24.685405839620522	25.48959306733104
25	25.378492019617116	24.13315508576188	24.8343379520599	25.654014942561105
26	25.17387731024307	24.89263516112322	24.959555229807073	24.973932298826636
27	25.782266090896496	24.37436128120968	24.369247825484425	25.474124802409403
28	25.079609751036575	24.360176227124008	25.11169820549209	25.448515816347328
29	25.167701182134007	24.461770355351618	24.958871340660906	25.41165712185347
30	24.91320222824794	24.62136903707645	25.225563046121945	25.239865688553664
31	25.713845289610454	24.29426974677381	24.249507354298043	25.74237760931769
32	25.465352903425654	24.462837722672052	24.20050007188348	25.871309302018812
33	25.04157427937916	24.180371604396647	24.758113283612175	26.019940832612022
34	25.758438082195433	24.177015572639462	24.928228317689552	25.136318027475546
35	26.121728484904178	24.096551611700793	24.86355178140421	24.91816812199082
36	25.07044132360025	24.704932004298367	24.572349649831402	25.65227702226998
37	25.984654011240483	24.372613768408073	24.41596611254713	25.22676610780431
38	25.190669408778465	24.265540177496156	24.930168788097752	25.61362162562763
39	25.889676484646824	24.161642810296467	24.328902952804775	25.619777752251938
40	26.09860732036819	24.23913212633938	24.748612915393583	24.91364763789885
41	25.053134726545128	24.680302356602933	25.080257892396617	25.186305024455315
42	26.203128902142332	24.833650630382408	24.56220047294428	24.40101999453098
43	25.499267466858157	23.915780549818553	24.91739086293664	25.66756112038665
44	25.17776089816033	24.573355318005817	24.50836467384256	25.740519109991293
45	25.266909980176745	24.755729292105897	24.52318580135984	25.45417492635752
46	25.06550923798824	24.446876750741794	24.776646104868135	25.710967906401823
47	25.32923282138689	24.35415084202531	24.995238712785074	25.321377623802732
48	25.975233804152897	24.921818262660995	24.118510174741743	24.98443775844437
49	25.175239748132	24.98353016850731	24.27167774412588	25.569552339234807
50	25.35839179624597	25.07271583356244	24.255186074723344	25.313706295468236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	21.5
2	24.0
3	65.0
4	106.0
5	138.5
6	171.0
7	157.5
8	144.0
9	187.5
10	231.0
11	379.0
12	527.0
13	1010.5
14	1494.0
15	1921.0
16	2348.0
17	2431.5
18	2515.0
19	2554.0
20	2593.0
21	2991.0
22	3389.0
23	3975.0
24	4561.0
25	5702.0
26	6843.0
27	8590.5
28	10338.0
29	12108.5
30	13879.0
31	15996.0
32	18113.0
33	21444.5
34	24776.0
35	29159.5
36	33543.0
37	39630.0
38	45717.0
39	50462.5
40	55208.0
41	60800.0
42	66392.0
43	69522.5
44	72653.0
45	79799.0
46	86945.0
47	93556.0
48	100167.0
49	104142.0
50	108117.0
51	105274.5
52	102432.0
53	100829.5
54	99227.0
55	100227.5
56	101228.0
57	98300.5
58	95373.0
59	87604.0
60	79835.0
61	70247.0
62	60659.0
63	52859.5
64	45060.0
65	38443.5
66	31827.0
67	27532.0
68	23237.0
69	20902.5
70	18568.0
71	14634.5
72	10701.0
73	9039.0
74	7377.0
75	5913.5
76	4450.0
77	3948.5
78	3447.0
79	2826.5
80	2206.0
81	1770.0
82	1334.0
83	1107.0
84	880.0
85	646.0
86	412.0
87	281.5
88	151.0
89	118.5
90	86.0
91	68.0
92	50.0
93	39.0
94	28.0
95	29.0
96	30.0
97	23.0
98	16.0
99	15.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009707723420290328
2	4.4462855360108373E-4
3	7.410475893351395E-5
4	0.0
5	0.0
6	0.0
7	7.410475893351395E-5
8	0.0
9	8.892571072021675E-4
10	5.928380714681116E-4
11	7.410475893351395E-5
12	7.410475893351395E-5
13	4.4462855360108373E-4
14	2.964190357340558E-4
15	0.004890914089611921
16	9.633618661356813E-4
17	0.0
18	7.410475893351395E-5
19	0.0
20	7.410475893351395E-4
21	0.0
22	1.482095178670279E-4
23	8.892571072021675E-4
24	2.964190357340558E-4
25	5.187333125345977E-4
26	0.005187333125345976
27	0.004446285536010837
28	0.0034088189109416417
29	0.0012597809018697372
30	0.002964190357340558
31	0.006965847339750311
32	0.002593666562672988
33	0.0036311331877421837
34	0.0017044094554708208
35	0.0020749332501383906
36	0.006743533062949769
37	0.0024454570448059604
38	0.008596152036287618
39	0.0034088189109416417
40	0.0020749332501383906
41	0.0030382951162740723
42	0.0016303046965373067
43	0.0010374666250691953
44	0.0011856761429362233
45	0.0011856761429362233
46	9.633618661356813E-4
47	0.0011856761429362233
48	0.001556199937603793
49	2.964190357340558E-4
50	7.410475893351395E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1349441.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.34921452508727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57394845050871	49.2292371469816
2	11.33564408165292	13.681944329274156
3	3.126093860567841	5.659719270509008
4	1.3256420077917241	3.200058156467607
5	0.7048424618752295	2.1268344469049434
6	0.4465060433738282	1.616777339898905
7	0.2783571182588567	1.175904341107218
8	0.19695767062352368	0.9508992573456408
9	0.14129507149465254	0.7674341922871496
>10	0.7480389487483836	8.078186736236495
>50	0.055344137847037074	2.303111641295619
>100	0.05905289758324691	7.345131322835305
>500	0.006794757195148771	2.769980821729097
>1k	0.00148249247894155	1.094780997127286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1408	0.10433950057838763	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1398	0.10359845298905251	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	7.410475893351395E-5
13	0.0	0.0	0.0	0.0	7.410475893351395E-5
14	0.0	0.0	0.0	7.410475893351395E-5	2.2231427680054186E-4
15	0.0	0.0	0.0	7.410475893351395E-5	2.964190357340558E-4
16	0.0	0.0	0.0	1.482095178670279E-4	2.964190357340558E-4
17	0.0	0.0	0.0	1.482095178670279E-4	2.964190357340558E-4
18	0.0	0.0	0.0	1.482095178670279E-4	3.7052379466756975E-4
19	0.0	0.0	0.0	1.482095178670279E-4	3.7052379466756975E-4
20	0.0	0.0	0.0	2.2231427680054186E-4	4.4462855360108373E-4
21	0.0	0.0	0.0	2.2231427680054186E-4	4.4462855360108373E-4
22	0.0	0.0	0.0	2.2231427680054186E-4	4.4462855360108373E-4
23	0.0	0.0	0.0	4.4462855360108373E-4	5.187333125345977E-4
24	0.0	0.0	0.0	7.410475893351395E-4	5.187333125345977E-4
25	0.0	0.0	0.0	0.0011115713840027092	5.187333125345977E-4
26	0.0	0.0	0.0	0.001482095178670279	6.669428304016255E-4
27	0.0	0.0	0.0	0.0020749332501383906	6.669428304016255E-4
28	0.0	0.0	0.0	0.0036311331877421837	6.669428304016255E-4
29	0.0	0.0	0.0	0.009855932938157355	6.669428304016255E-4
30	0.0	0.0	0.0	0.02030470394778282	6.669428304016255E-4
31	0.0	0.0	0.0	0.0403870936187651	6.669428304016255E-4
32	0.0	0.0	0.0	0.06291494033455335	7.410475893351395E-4
33	0.0	0.0	0.0	0.0834419585591367	7.410475893351395E-4
34	0.0	0.0	0.0	0.10915630990906605	7.410475893351395E-4
35	0.0	0.0	0.0	0.13524118505366295	7.410475893351395E-4
36	0.0	0.0	0.0	0.17496133584202644	7.410475893351395E-4
37	0.0	0.0	0.0	0.22668645757761918	7.410475893351395E-4
38	0.0	0.0	0.0	0.30360719735060665	7.410475893351395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1540	0.0	26.71388	1
TAGATCG	45	0.0013984215	24.444075	5
GGACCGT	85	6.115102E-6	20.705568	6
TAGGACG	300	0.0	19.066376	4
GGTATCA	725	0.0	18.813507	1
CGCAATA	345	0.0	18.494528	36
GTATAGA	180	1.4551915E-11	18.333055	1
AGCGAAA	540	0.0	17.110216	17
AACGCAG	2395	0.0	17.085335	6
GTATTAC	105	3.775357E-5	16.761652	1
GTCTAGC	105	3.775357E-5	16.761652	1
ATCAACG	2440	0.0	16.589912	3
ACGGACC	625	0.0	16.543749	8
CAACGCA	2465	0.0	16.510906	5
GTCTAGT	80	0.0019887132	16.49975	1
GTATAGT	120	6.408567E-6	16.49975	1
TCAACGC	2465	0.0	16.421658	4
TAAGACT	215	1.2732926E-11	16.371845	4
CAATACG	365	0.0	16.274933	38
ATACGAA	395	0.0	16.151655	40
>>END_MODULE