FastQCFastQC Report
Thu 2 Feb 2017
SRR4064042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660464
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16700.25285254003246205No Hit
TATCAACGCAGAGTACTTTTTTTTT12000.18169044792751762No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9740.1474720802345018No Hit
GCGCAAGACGGACCAGAGCGAAAGC7740.11719033891324888No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7730.1170389302066426No Hit
GTATCTGATCGTCTTCGAACCTCCG7480.11325371254148597No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7030.10644032074420408No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6970.10553186850456649No Hit
GAATAGGACCGCGGTTCTATTTTGT6960.10538045979796022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG402.7638086E-416.6204931
TAATACC508.721993E-515.1958784
TAGACTG508.727631E-515.1947275
TCAATAC456.776723E-414.7715333
CGGTCCA1203.6379788E-1214.25045510
TAGATCT400.00528434314.2461374
CCTATGG400.00528434314.2461371
AATACCT400.005286999514.2450575
CAATACT551.9619714E-413.8144364
TCCATTA1500.013.3004258
ATAAAGG500.001502733213.2943793
AAGACAA957.3949195E-812.995495
GTATTAG1251.0186341E-1012.9164961
CACTCTA1207.366907E-1012.6670719
AAGGCCG604.0984628E-412.6622735
CGCGGTC1001.4369289E-712.35039410
TCTAGGG701.0933734E-412.2091253
CGGTCGG550.003064496712.09129510
GTGTTAC550.003071461812.0876311
GCCTTAG550.003071461812.0876311
Produced by FastQC (version 0.11.5)