##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064042_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660464
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13908555197558	32.0	32.0	32.0	32.0	32.0
2	30.700396993628722	32.0	32.0	32.0	32.0	32.0
3	30.71762124809225	32.0	32.0	32.0	32.0	32.0
4	30.746706860631313	32.0	32.0	32.0	32.0	32.0
5	30.64258914944645	32.0	32.0	32.0	32.0	32.0
6	34.27064457714577	36.0	36.0	36.0	32.0	36.0
7	34.19757170716345	36.0	36.0	36.0	32.0	36.0
8	34.14322809418833	36.0	36.0	36.0	32.0	36.0
9	34.30068709271058	36.0	36.0	36.0	32.0	36.0
10	33.9692610043848	36.0	36.0	36.0	32.0	36.0
11	34.28678171709586	36.0	36.0	36.0	32.0	36.0
12	34.072545967683325	36.0	36.0	36.0	32.0	36.0
13	34.17571888853897	36.0	36.0	36.0	32.0	36.0
14	34.068784975411226	36.0	36.0	36.0	32.0	36.0
15	33.98804628261343	36.0	36.0	36.0	32.0	36.0
16	33.99693094551709	36.0	36.0	36.0	32.0	36.0
17	33.91607415392815	36.0	36.0	36.0	32.0	36.0
18	33.91284309212917	36.0	36.0	36.0	32.0	36.0
19	33.918825250127185	36.0	36.0	36.0	32.0	36.0
20	33.91050079943797	36.0	36.0	36.0	32.0	36.0
21	33.8847840306209	36.0	36.0	36.0	32.0	36.0
22	33.875411831681966	36.0	36.0	36.0	32.0	36.0
23	33.82927002834371	36.0	36.0	36.0	32.0	36.0
24	33.79599039463165	36.0	36.0	36.0	32.0	36.0
25	33.40048208532183	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	7.0
5	9.0
6	24.0
7	11.0
8	28.0
9	44.0
10	53.0
11	12.0
12	44.0
13	44.0
14	64.0
15	109.0
16	190.0
17	267.0
18	347.0
19	487.0
20	730.0
21	1144.0
22	1751.0
23	2789.0
24	4152.0
25	6069.0
26	8723.0
27	11248.0
28	15521.0
29	20645.0
30	26819.0
31	35399.0
32	48986.0
33	67679.0
34	137486.0
35	269581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09240507671265	17.315140618396796	11.570287992414316	26.022166312476237
2	16.760899058786478	19.831653604351143	36.87055588531004	26.536891451552336
3	18.681666426932946	23.903823878992515	28.580902559599064	28.833607134475475
4	12.785892848760886	15.212792857997279	35.57570737896919	36.42560691427265
5	14.859434102258929	36.390410689246856	33.28730076529144	15.462854443202776
6	34.09794360822557	35.384021321057574	16.8578697113783	13.660165359338563
7	30.09913255230734	30.441689584570092	21.020613997891957	18.438563865230613
8	28.38823317375046	31.96241497706217	19.773938566904402	19.87541328228297
9	27.50669152921324	13.915805641939945	18.72991973194499	39.84758309690183
10	16.14057997800313	26.24504994743173	31.33250718837014	26.281862886194997
11	37.249519793011096	20.97141177825041	22.453388110256736	19.32568031848176
12	24.64462156343787	23.664575477486512	28.577986307533582	23.11281665154203
13	29.308905584113177	19.238140255033944	25.46748900493736	25.985465155915517
14	23.99832738930317	19.36455943979662	25.011590101295972	31.625523069604238
15	25.323631581897537	26.81782855896268	22.279790391266605	25.578749467873173
16	25.964727288703855	25.48885929548509	23.46842772669633	25.07798568911473
17	24.14244463499547	25.54168169023473	25.092186633619868	25.223687041149933
18	25.092010503364502	24.654269302508112	25.950068108227686	24.303652085899703
19	25.68984012315002	24.528945103695712	25.368480382055974	24.41273439109829
20	26.03443155508685	23.987841982132686	24.749687867437604	25.22803859534286
21	27.226032845603843	23.821926463302106	24.248443553531303	24.703597137562745
22	26.191064234056643	23.755221772879963	24.861017883956563	25.192696109106834
23	24.709713296896286	23.79858504646557	25.337032597874693	26.154669058763453
24	24.89829006083656	24.73676634366974	25.164061730546315	25.200881864947384
25	25.0462888606728	24.25854487822469	25.166897479643506	25.528268781459
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	161.0
2	233.5
3	306.0
4	306.0
5	306.0
6	809.5
7	1313.0
8	1313.0
9	1313.0
10	1375.5
11	1438.0
12	1438.0
13	1438.0
14	1504.5
15	1571.0
16	1571.0
17	1571.0
18	2832.0
19	4093.0
20	4093.0
21	4093.0
22	6650.0
23	9207.0
24	9207.0
25	9207.0
26	13488.0
27	17769.0
28	17769.0
29	17769.0
30	21792.5
31	25816.0
32	25816.0
33	25816.0
34	32841.5
35	39867.0
36	39867.0
37	39867.0
38	45974.5
39	52082.0
40	52082.0
41	52082.0
42	60358.5
43	68635.0
44	68635.0
45	68635.0
46	76976.0
47	85317.0
48	85317.0
49	85317.0
50	88118.5
51	90920.0
52	90920.0
53	90920.0
54	84544.5
55	78169.0
56	78169.0
57	78169.0
58	71949.5
59	65730.0
60	65730.0
61	65730.0
62	57819.5
63	49909.0
64	49909.0
65	49909.0
66	41322.0
67	32735.0
68	32735.0
69	32735.0
70	24713.5
71	16692.0
72	16692.0
73	16692.0
74	12882.5
75	9073.0
76	9073.0
77	9073.0
78	7259.5
79	5446.0
80	5446.0
81	5446.0
82	3735.5
83	2025.0
84	2025.0
85	2025.0
86	1594.0
87	1163.0
88	1163.0
89	1163.0
90	816.5
91	470.0
92	470.0
93	470.0
94	326.0
95	182.0
96	182.0
97	182.0
98	278.5
99	375.0
100	375.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04133457690351026
2	0.006056348264250587
3	0.0013626783594563822
4	0.004996487318006735
5	0.009387339809588412
6	0.012718331354926232
7	0.020591584098451996
8	0.0304331500278592
9	0.04557402068848567
10	0.056021221444317926
11	0.05026769059327988
12	0.05950362169626203
13	0.05980643910947455
14	0.06328883936141863
15	0.05708108239056178
16	0.061623343588749734
17	0.05904939557644323
18	0.07343322270403838
19	0.0690423702124567
20	0.07388744882385717
21	0.07070786598512561
22	0.0737360401172509
23	0.07767266648901379
24	0.07555294459652609
25	0.07237336175779452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	660464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.33503045539413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0955678490823	43.76755655815662
2	12.118832245855735	13.411919028164792
3	3.6763376900523967	6.10290774130088
4	1.6427533020718008	3.636072160033695
5	0.8522717351667733	2.3580241210862503
6	0.5621295367844362	1.8663273022706026
7	0.38074729665012336	1.474806427916049
8	0.2595106626094421	1.1488024335194393
9	0.20057205222407246	0.9988794556487957
>10	1.0291546629046446	10.18997897006316
>50	0.09106151442002097	3.576398319766292
>100	0.08420441511674158	8.832472907273523
>500	0.006308474096683344	2.2002639356753253
>1k	5.485629649289865E-4	0.43559063912452123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1670	0.25285254003246205	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1200	0.18169044792751762	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	974	0.1474720802345018	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	774	0.11719033891324888	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	773	0.1170389302066426	No Hit
GTATCTGATCGTCTTCGAACCTCCG	748	0.11325371254148597	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	703	0.10644032074420408	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	697	0.10553186850456649	No Hit
GAATAGGACCGCGGTTCTATTTTGT	696	0.10538045979796022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.514087066062647E-4
13	0.0	0.0	0.0	0.0	4.5422611981879406E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	40	2.7638086E-4	16.620493	1
TAATACC	50	8.721993E-5	15.195878	4
TAGACTG	50	8.727631E-5	15.194727	5
TCAATAC	45	6.776723E-4	14.771533	3
CGGTCCA	120	3.6379788E-12	14.250455	10
TAGATCT	40	0.005284343	14.246137	4
CCTATGG	40	0.005284343	14.246137	1
AATACCT	40	0.0052869995	14.245057	5
CAATACT	55	1.9619714E-4	13.814436	4
TCCATTA	150	0.0	13.300425	8
ATAAAGG	50	0.0015027332	13.294379	3
AAGACAA	95	7.3949195E-8	12.99549	5
GTATTAG	125	1.0186341E-10	12.916496	1
CACTCTA	120	7.366907E-10	12.667071	9
AAGGCCG	60	4.0984628E-4	12.662273	5
CGCGGTC	100	1.4369289E-7	12.350394	10
TCTAGGG	70	1.0933734E-4	12.209125	3
CGGTCGG	55	0.0030644967	12.091295	10
GTGTTAC	55	0.0030714618	12.087631	1
GCCTTAG	55	0.0030714618	12.087631	1
>>END_MODULE