FastQCFastQC Report
Thu 2 Feb 2017
SRR4064042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660464
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7660.11597906926039876No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7430.11249666900845466No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6890.10432059885171636No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6610.10008115506674095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC502.3597859E-630.7992083
ACTTCGG508.332973E-526.40131832
GTATCAA12050.023.0052971
GTTCGCA500.00257959621.9994349
CGGACCG500.00257959621.99943421
TAGACAG856.1078827E-620.7053475
GTATATA1001.1213197E-619.800991
GTCCTAT2050.019.3180371
TACGAAT1950.019.17899141
CGCAATA1851.8189894E-1219.02797536
TGCGATC708.1194023E-418.85665741
CGAACGT1051.7906204E-618.8566554
TATTCCA2250.018.57735
ATTACAC600.00740973518.332863
TAGCCTA600.00740973518.332865
TCTAAAC600.00740973518.332863
CTATTCC2050.018.2434314
CGACGGT2350.017.7867747
AGGCGAA750.001290252817.59954618
TGTAGGA1002.4807987E-517.5995462
Produced by FastQC (version 0.11.5)