FastQCFastQC Report
Thu 2 Feb 2017
SRR4064041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842892
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28640.339782558145053No Hit
TATCAACGCAGAGTACTTTTTTTTT22050.261599350806509No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18100.21473688206792804No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12520.14853623002709718No Hit
GTACATGGGGTGGTATCAACGCAAA11320.13429953066347763No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9850.11685957394304372No Hit
GTCCTAAAGTGTGTATTTCTCATTT9700.11507998652259127No Hit
GGTATCAACGCAGAGTACTTTTTTT9590.1137749557475928No Hit
GATATACACTGTTCTACAAATCCCG9020.10701252354987352No Hit
GTCCTACAGTGGACATTTCTAAATT8440.10013145219079077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC350.00216230616.2933519
GCGCTAT901.3460522E-1015.835114511
GCTATCG851.0149961E-915.64974913
CGCTATC851.0149961E-915.64974912
TCCAACG1450.014.41883918
GTTATAG400.005295401414.24231
TCGGTCT1101.8371793E-1013.82302117
TAGGGTG551.9615395E-413.8156355
CGGTCTC1153.7471182E-1013.2220218
TAGGACA1750.013.0246234
CCAACGA905.364527E-712.67260619
CTATCGG1051.9837898E-812.66884414
CGGTGCG905.383881E-712.66884414
TGCGCTA1051.985427E-812.66809310
CGGTCCA604.084687E-412.66809310
CGCCGGT604.0928804E-412.6650847
GTCCGGT1155.298716E-912.39269911
TTAGGAC3450.012.3846093
CGTTTCC853.9296956E-612.29623114
TTGTGCG1103.8107828E-812.0893987
Produced by FastQC (version 0.11.5)