##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064041_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842892
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197673011489016	32.0	32.0	32.0	32.0	32.0
2	30.822664113551912	32.0	32.0	32.0	32.0	32.0
3	30.858318740716484	32.0	32.0	32.0	32.0	32.0
4	30.87411435866042	32.0	32.0	32.0	32.0	32.0
5	30.786513574692844	32.0	32.0	32.0	32.0	32.0
6	34.43193789951738	36.0	36.0	36.0	32.0	36.0
7	34.35924887174158	36.0	36.0	36.0	32.0	36.0
8	34.30372455783185	36.0	36.0	36.0	32.0	36.0
9	34.44101616814491	36.0	36.0	36.0	32.0	36.0
10	34.16396169378758	36.0	36.0	36.0	32.0	36.0
11	34.44736929523592	36.0	36.0	36.0	32.0	36.0
12	34.272871257527655	36.0	36.0	36.0	32.0	36.0
13	34.3354712110211	36.0	36.0	36.0	32.0	36.0
14	34.255318593603924	36.0	36.0	36.0	32.0	36.0
15	34.19373419133175	36.0	36.0	36.0	32.0	36.0
16	34.188344414231004	36.0	36.0	36.0	32.0	36.0
17	34.132809422796754	36.0	36.0	36.0	32.0	36.0
18	34.121467518970405	36.0	36.0	36.0	32.0	36.0
19	34.1218151317132	36.0	36.0	36.0	32.0	36.0
20	34.081412565310856	36.0	36.0	36.0	32.0	36.0
21	34.066829439596056	36.0	36.0	36.0	32.0	36.0
22	34.043019746302015	36.0	36.0	36.0	32.0	36.0
23	33.98925010558885	36.0	36.0	36.0	32.0	36.0
24	33.981950237990155	36.0	36.0	36.0	32.0	36.0
25	33.57278749827973	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	9.0
5	4.0
6	25.0
7	9.0
8	37.0
9	57.0
10	77.0
11	24.0
12	42.0
13	45.0
14	96.0
15	153.0
16	267.0
17	345.0
18	444.0
19	540.0
20	843.0
21	1318.0
22	1946.0
23	2987.0
24	4496.0
25	6531.0
26	9336.0
27	12435.0
28	17013.0
29	23316.0
30	30554.0
31	42209.0
32	59237.0
33	84121.0
34	177429.0
35	366944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.676540472793825	17.271287946582227	11.384271380520932	25.667900200103016
2	15.628133012354672	19.89217395018503	39.44489991564225	25.034793121818044
3	19.03734242918582	24.524751594245885	28.965030401898268	27.47287557467003
4	12.087995529511984	15.667233387237042	36.78139100041407	35.463380082836906
5	13.574466822887313	37.782813751371876	34.3618188829235	14.28090054281731
6	33.09231134314191	36.30564784053156	17.471167536782154	13.130873279544375
7	29.21011255287968	31.026503622221036	21.155194816752996	18.60818900814629
8	27.57204332212224	34.30071515206179	19.65220301488931	18.47503851092666
9	27.667221361034816	13.858298931995735	18.557731339598767	39.91674836737068
10	15.302474206245579	27.800753039935806	32.95012653539905	23.946646218419566
11	36.81441748558121	20.959029148570227	23.12336123122649	19.103192134622073
12	24.30640956853286	23.67454548044462	30.608075704986064	21.41096924603646
13	30.29446888525376	20.32067977369367	25.163877407116352	24.220973933936214
14	22.7620821506709	20.01783045398805	26.44177976896385	30.7783076263772
15	24.57364865586707	28.599696832649013	23.20595319067612	23.620701320807797
16	24.371725076152735	26.069402862820606	25.75173376575584	23.807138295270814
17	22.812169846036966	26.305718118255957	26.734013128998946	24.148098906708135
18	23.26227460738827	25.905963139374194	27.91995327124995	22.911808981987587
19	25.144750573125602	25.016769340540492	25.94433647429512	23.894143612038786
20	25.27416817547713	25.290077468760202	26.213410109524798	23.222344246237867
21	25.85513818976161	24.536043308956216	25.3284974831418	24.280321018140373
22	24.940726713642057	25.531619344996052	25.963895830983574	23.563758110378316
23	23.94886943801975	25.439306797619672	26.07060253895583	24.541221225404755
24	24.46319473630473	25.666688036775582	26.1629494549435	23.707167771976188
25	24.39494657958567	25.38570856994281	26.188884707122682	24.030460143348833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.0
2	312.0
3	520.0
4	520.0
5	520.0
6	1422.0
7	2324.0
8	2324.0
9	2324.0
10	2310.5
11	2297.0
12	2297.0
13	2297.0
14	2353.5
15	2410.0
16	2410.0
17	2410.0
18	4366.5
19	6323.0
20	6323.0
21	6323.0
22	10122.5
23	13922.0
24	13922.0
25	13922.0
26	22083.0
27	30244.0
28	30244.0
29	30244.0
30	39424.5
31	48605.0
32	48605.0
33	48605.0
34	54661.0
35	60717.0
36	60717.0
37	60717.0
38	67973.0
39	75229.0
40	75229.0
41	75229.0
42	83636.0
43	92043.0
44	92043.0
45	92043.0
46	101691.5
47	111340.0
48	111340.0
49	111340.0
50	110561.0
51	109782.0
52	109782.0
53	109782.0
54	100535.5
55	91289.0
56	91289.0
57	91289.0
58	81556.0
59	71823.0
60	71823.0
61	71823.0
62	63119.5
63	54416.0
64	54416.0
65	54416.0
66	44312.5
67	34209.0
68	34209.0
69	34209.0
70	25776.5
71	17344.0
72	17344.0
73	17344.0
74	13323.0
75	9302.0
76	9302.0
77	9302.0
78	7074.5
79	4847.0
80	4847.0
81	4847.0
82	3355.5
83	1864.0
84	1864.0
85	1864.0
86	1389.0
87	914.0
88	914.0
89	914.0
90	642.5
91	371.0
92	371.0
93	371.0
94	257.0
95	143.0
96	143.0
97	143.0
98	326.5
99	510.0
100	510.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038676366604499746
2	0.006287875552265296
3	0.0020168657431794343
4	0.003677814002268381
5	0.007948823811354243
6	0.010914802845441645
7	0.02099913156133882
8	0.03250713021359795
9	0.04579471628630951
10	0.052201230999938314
11	0.04923525196585091
12	0.05504857087266222
13	0.0586077457135671
14	0.06086188977947353
15	0.05374354009766376
16	0.06003141564992905
17	0.05718407577720515
18	0.06928527023628175
19	0.06892935275219127
20	0.07320036256127713
21	0.06786160029991979
22	0.07201397094764217
23	0.07782728985445347
24	0.07047166184991671
25	0.07248852759309615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	842892.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.847300519378564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9135451003628	45.26154502531827
2	14.480892868166872	17.043229088038835
3	4.299021182940796	7.589573744750741
4	1.699319944317437	4.000015657672875
5	0.8032025246029199	2.363315017161579
6	0.4421297654028387	1.5610885903933918
7	0.25873305953925335	1.0658019476303298
8	0.17852356113342754	0.8404503721446769
9	0.1245450295831956	0.6596224905669485
>10	0.6603617558639816	7.589171462890114
>50	0.07755896818571506	3.2194179029716885
>100	0.05892630558764379	6.65527409810156
>500	0.002227454840305074	1.0476843281052657
>1k	0.0010124794728659427	1.103810274253762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2864	0.339782558145053	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2205	0.261599350806509	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1810	0.21473688206792804	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1252	0.14853623002709718	No Hit
GTACATGGGGTGGTATCAACGCAAA	1132	0.13429953066347763	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	985	0.11685957394304372	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	970	0.11507998652259127	No Hit
GGTATCAACGCAGAGTACTTTTTTT	959	0.1137749557475928	No Hit
GATATACACTGTTCTACAAATCCCG	902	0.10701252354987352	No Hit
GTCCTACAGTGGACATTTCTAAATT	844	0.10013145219079077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGAC	35	0.002162306	16.29335	19
GCGCTAT	90	1.3460522E-10	15.8351145	11
GCTATCG	85	1.0149961E-9	15.649749	13
CGCTATC	85	1.0149961E-9	15.649749	12
TCCAACG	145	0.0	14.418839	18
GTTATAG	40	0.0052954014	14.2423	1
TCGGTCT	110	1.8371793E-10	13.823021	17
TAGGGTG	55	1.9615395E-4	13.815635	5
CGGTCTC	115	3.7471182E-10	13.22202	18
TAGGACA	175	0.0	13.024623	4
CCAACGA	90	5.364527E-7	12.672606	19
CTATCGG	105	1.9837898E-8	12.668844	14
CGGTGCG	90	5.383881E-7	12.668844	14
TGCGCTA	105	1.985427E-8	12.668093	10
CGGTCCA	60	4.084687E-4	12.668093	10
CGCCGGT	60	4.0928804E-4	12.665084	7
GTCCGGT	115	5.298716E-9	12.392699	11
TTAGGAC	345	0.0	12.384609	3
CGTTTCC	85	3.9296956E-6	12.296231	14
TTGTGCG	110	3.8107828E-8	12.089398	7
>>END_MODULE