FastQCFastQC Report
Thu 2 Feb 2017
SRR4064041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842892
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8570.10167376128851621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG407.032348E-427.4986655
ACCGAGT500.00258052321.9989328
ATACCGT1351.1277734E-1021.1841566
CGTCTTA856.1061037E-620.70733515
TAGGACC5000.020.6789954
GTCCTAA3300.020.6681181
GTAGGAC6600.020.331143
GATATAC4550.020.3091161
CTAGGAC1750.020.1121163
ATAATAC1001.1237717E-619.7978653
TAGGACG5850.019.5546054
GCACTAC808.9796195E-519.251351
TGTAGGA7100.018.899372
CGTATGC708.119946E-418.85734621
GCGCATG708.126427E-418.85510841
GTCTTAC1456.2937033E-918.2081741
CTGTAGG7400.018.1364081
AATCACG3650.018.0834634
GTCCTAC6500.017.939721
AGGACGT6750.017.9250565