##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842892
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22279248112451	32.0	32.0	32.0	32.0	32.0
2	31.340257114790507	32.0	32.0	32.0	32.0	32.0
3	31.43499048513926	32.0	32.0	32.0	32.0	32.0
4	31.530690764653123	32.0	32.0	32.0	32.0	32.0
5	31.46339270036968	32.0	32.0	32.0	32.0	32.0
6	35.03904296161311	36.0	36.0	36.0	36.0	36.0
7	35.07381016785069	36.0	36.0	36.0	36.0	36.0
8	35.007537145921425	36.0	36.0	36.0	36.0	36.0
9	35.13629504135761	36.0	36.0	36.0	36.0	36.0
10	34.9770812868078	36.0	36.0	36.0	36.0	36.0
11	35.14165041310156	36.0	36.0	36.0	36.0	36.0
12	35.04813902611485	36.0	36.0	36.0	36.0	36.0
13	35.08001262320677	36.0	36.0	36.0	36.0	36.0
14	35.03070381496087	36.0	36.0	36.0	36.0	36.0
15	35.00387831418497	36.0	36.0	36.0	36.0	36.0
16	35.01713861325057	36.0	36.0	36.0	36.0	36.0
17	34.98307375084827	36.0	36.0	36.0	36.0	36.0
18	34.96591022337381	36.0	36.0	36.0	36.0	36.0
19	34.969825315698806	36.0	36.0	36.0	36.0	36.0
20	34.96185039127195	36.0	36.0	36.0	36.0	36.0
21	34.95534066048794	36.0	36.0	36.0	36.0	36.0
22	34.93109793425492	36.0	36.0	36.0	36.0	36.0
23	34.88213911153505	36.0	36.0	36.0	32.0	36.0
24	34.85681795532524	36.0	36.0	36.0	32.0	36.0
25	34.8271261324108	36.0	36.0	36.0	32.0	36.0
26	34.775316410643356	36.0	36.0	36.0	32.0	36.0
27	34.75964655021047	36.0	36.0	36.0	32.0	36.0
28	34.728094465245846	36.0	36.0	36.0	32.0	36.0
29	34.69314336830816	36.0	36.0	36.0	32.0	36.0
30	34.67364858131291	36.0	36.0	36.0	32.0	36.0
31	34.65429853409452	36.0	36.0	36.0	32.0	36.0
32	34.613315822193115	36.0	36.0	36.0	32.0	36.0
33	34.59744190240268	36.0	36.0	36.0	32.0	36.0
34	34.56695282432388	36.0	36.0	36.0	32.0	36.0
35	34.528014265172764	36.0	36.0	36.0	32.0	36.0
36	34.49479174081614	36.0	36.0	36.0	32.0	36.0
37	34.459260498379386	36.0	36.0	36.0	32.0	36.0
38	34.40787787759286	36.0	36.0	36.0	32.0	36.0
39	34.381789126009025	36.0	36.0	36.0	32.0	36.0
40	34.371918347783584	36.0	36.0	36.0	32.0	36.0
41	34.34846101279879	36.0	36.0	36.0	32.0	36.0
42	34.26800705191175	36.0	36.0	36.0	32.0	36.0
43	34.26864888977473	36.0	36.0	36.0	32.0	36.0
44	34.20364412048044	36.0	36.0	36.0	32.0	36.0
45	34.13320923677055	36.0	36.0	36.0	32.0	36.0
46	34.095843832899114	36.0	36.0	36.0	32.0	36.0
47	34.05681985355182	36.0	36.0	36.0	32.0	36.0
48	33.996322185997734	36.0	36.0	36.0	32.0	36.0
49	33.96515805109077	36.0	36.0	36.0	32.0	36.0
50	33.37206901951851	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	20.0
22	92.0
23	275.0
24	676.0
25	1720.0
26	3484.0
27	6401.0
28	10569.0
29	15979.0
30	22957.0
31	32422.0
32	47083.0
33	75351.0
34	154440.0
35	471411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.77553211811426	18.198267464473055	11.986910469844366	26.039289947568317
2	15.057024115868161	20.60117049813202	38.80036398624255	25.541441399757264
3	18.392576009415237	25.146608865461527	29.192574585740232	27.268240539383005
4	11.807918452186044	16.360458991187482	36.85122174608372	34.98040081054275
5	13.32424557357289	38.19113243452305	34.08087868908472	14.403743302819342
6	33.31387651086972	36.60635051702947	17.104326532936604	12.975446439164212
7	29.05879882452179	31.153019785476417	21.251264991558813	18.53691639844298
8	27.624654166844625	34.59470489695002	19.424196694238407	18.356444241966944
9	27.576404707668946	13.616410402429766	18.47582099468489	40.3313638952164
10	15.25312528547853	28.05941038080018	32.84963630776751	23.83782802595378
11	36.95135319827451	21.00174162288882	23.043165672470494	19.003739506366177
12	24.277250228973582	23.829743312310477	30.542584340579815	21.35042211813613
13	30.447698863905998	20.344577215478214	25.237516728201136	23.970207192414648
14	22.81448350318547	20.107250056353735	26.40344528942092	30.67482115103988
15	24.6969812019192	28.629700113424423	22.938902678050237	23.734416006606143
16	24.44710725662457	26.172728189492044	25.593289713306085	23.7868748405773
17	22.790701537088975	26.47005784845508	26.57671445452086	24.16252615993508
18	23.35853627574621	25.831216610451413	27.853423515021515	22.95682359878086
19	25.303716253090553	25.081979660502174	25.81872885256949	23.795575233837788
20	25.41850460681329	25.289186386706564	26.140907030877397	23.151401975602752
21	25.907826862753474	24.49803770827105	25.423660445229046	24.170474983746434
22	25.02948190155299	25.44175396552338	25.901600446084306	23.627163686839324
23	24.086089627459845	25.29123051789737	26.113402832401416	24.509277022241363
24	24.57752394149638	25.627366862501304	26.085790757490912	23.709318438511403
25	24.474094146372135	25.35002058421197	26.259522422554127	23.91636284686177
26	23.561836265205617	26.115632077810986	26.796051078289118	23.526480578694283
27	23.984571321451366	25.722018019899245	26.430095177529466	23.86331548111992
28	23.489980661311947	26.158245043719674	26.146618102435724	24.205156192532655
29	23.797213350480973	25.512412264674754	25.99896070121821	24.691413683626063
30	23.56904553996849	26.00407657700412	26.385514151749273	24.041363731278118
31	24.514527685595944	25.566329987103114	25.53453269248338	24.384609634817565
32	23.732847376950765	26.040309702280528	25.847754231356053	24.379088689412654
33	23.72277885545135	25.6652014260884	26.550871135947034	24.061148582513216
34	24.256236979104877	25.86821786360034	26.627404268243488	23.248140889051296
35	24.155121827369012	26.02776456239552	26.396028820474733	23.421084789760734
36	23.632858151358214	26.276673370232608	26.137381575278674	23.953086903130504
37	24.172026324312974	26.06069018865283	25.524309176611844	24.242974310422355
38	24.072013155428156	26.403874491900066	25.64798314282799	23.876129209843793
39	24.51590246032245	25.431478886691337	25.923258631060786	24.12936002192543
40	25.054011567551537	25.660744475752633	26.006110040041523	23.279133916654306
41	24.001808099431234	25.743824106568713	26.739346569000823	23.51502122499923
42	24.454845292331058	26.678886674259683	25.818147304479876	23.048120728929387
43	23.71948116044851	25.95526533401512	26.222681493591622	24.102572011944748
44	23.539838127575972	26.347097946816056	25.72091599575743	24.392147929850537
45	23.61837125481384	26.627214722820035	25.631464427998225	24.122949594367896
46	23.914977826182902	25.847030322012703	25.45373870250544	24.78425314929896
47	24.380906054799883	25.836739294209764	25.81467222693487	23.967682424055475
48	24.099986237596042	26.164584114389307	25.22577460978839	24.509655038226263
49	24.126697133203304	26.250575399932615	25.675531384803747	23.947196082060334
50	23.770649342020967	26.903939332102638	25.1418937837235	24.183517542152895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	19.5
2	16.0
3	56.5
4	97.0
5	115.0
6	133.0
7	153.5
8	174.0
9	230.0
10	286.0
11	467.0
12	648.0
13	1121.5
14	1595.0
15	2177.0
16	2759.0
17	3067.5
18	3376.0
19	3569.5
20	3763.0
21	4080.5
22	4398.0
23	5006.5
24	5615.0
25	6148.5
26	6682.0
27	8558.0
28	10434.0
29	12606.0
30	14778.0
31	17107.5
32	19437.0
33	23075.0
34	26713.0
35	30890.0
36	35067.0
37	37352.0
38	39637.0
39	40738.5
40	41840.0
41	44159.0
42	46478.0
43	47112.5
44	47747.0
45	50747.0
46	53747.0
47	56148.0
48	58549.0
49	59406.5
50	60264.0
51	58746.0
52	57228.0
53	55994.5
54	54761.0
55	53838.5
56	52916.0
57	50455.0
58	47994.0
59	44084.0
60	40174.0
61	35226.5
62	30279.0
63	26424.5
64	22570.0
65	19441.5
66	16313.0
67	14222.5
68	12132.0
69	10846.0
70	9560.0
71	7524.5
72	5489.0
73	4520.0
74	3551.0
75	2842.0
76	2133.0
77	1772.0
78	1411.0
79	1160.5
80	910.0
81	718.5
82	527.0
83	424.5
84	322.0
85	245.0
86	168.0
87	125.5
88	83.0
89	60.0
90	37.0
91	32.0
92	27.0
93	25.0
94	23.0
95	19.5
96	16.0
97	11.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009609772070443189
2	3.559174840904885E-4
3	3.559174840904885E-4
4	0.0
5	0.0
6	0.0
7	1.1863916136349615E-4
8	0.0
9	0.001423669936361954
10	0.0010677524522714652
11	0.0
12	0.0
13	4.745566454539846E-4
14	2.372783227269923E-4
15	0.0047455664545398455
16	8.30474129544473E-4
17	0.0
18	1.1863916136349615E-4
19	0.0
20	0.0011863916136349614
21	0.0
22	2.372783227269923E-4
23	3.559174840904885E-4
24	4.745566454539846E-4
25	0.0015423090977254499
26	0.0034405356795413886
27	0.0054574014227208225
28	0.002610061549996915
29	0.0018982265818159383
30	0.003321896518177892
31	0.006287875552265296
32	0.0021355049045429304
33	0.0032032573568143963
34	0.0018982265818159383
35	0.0022541440659064266
36	0.006762432197719281
37	0.002491422388633419
38	0.0054574014227208225
39	0.004152370647722365
40	0.0020168657431794343
41	0.0021355049045429304
42	0.001423669936361954
43	0.0013050307749984575
44	7.11834968180977E-4
45	0.0011863916136349614
46	7.11834968180977E-4
47	8.30474129544473E-4
48	0.0018982265818159383
49	0.0
50	9.491132909079692E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	842892.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.778478921851566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22781290540362	49.892509164898335
2	13.72800764691674	17.511028926957927
3	4.139702570542672	7.920717994142719
4	1.6589948457034667	4.2323267119263575
5	0.7386994827674996	2.3556564695634816
6	0.3948464623556777	1.5109624066031548
7	0.21162103827667178	0.9447807550404338
8	0.15154587874239617	0.773229250645231
9	0.11103708522648222	0.6373598759694928
>10	0.5343259124385291	6.3900353739374625
>50	0.06320486002588856	2.8379555161493677
>100	0.03964036307261793	4.7529005966558
>500	5.609485277568859E-4	0.24053695751028933
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	857	0.10167376128851621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1863916136349616E-4	0.0
10	0.0	0.0	0.0	1.1863916136349616E-4	0.0
11	0.0	0.0	0.0	1.1863916136349616E-4	0.0
12	0.0	0.0	0.0	1.1863916136349616E-4	0.0
13	0.0	0.0	0.0	1.1863916136349616E-4	0.0
14	0.0	0.0	0.0	1.1863916136349616E-4	0.0
15	0.0	0.0	0.0	1.1863916136349616E-4	0.0
16	0.0	0.0	0.0	1.1863916136349616E-4	0.0
17	0.0	0.0	0.0	2.3727832272699232E-4	0.0
18	0.0	0.0	0.0	2.3727832272699232E-4	0.0
19	0.0	0.0	0.0	2.3727832272699232E-4	0.0
20	0.0	0.0	0.0	3.559174840904885E-4	0.0
21	0.0	0.0	0.0	5.931958068174808E-4	0.0
22	0.0	0.0	0.0	0.00154230909772545	0.0
23	0.0	0.0	0.0	0.0026100615499969155	0.0
24	1.1863916136349616E-4	0.0	0.0	0.003796453163631877	0.0
25	1.1863916136349616E-4	0.0	0.0	0.005813318906811311	0.0
26	1.1863916136349616E-4	0.0	0.0	0.007474267165900258	0.0
27	1.1863916136349616E-4	0.0	0.0	0.009135215424989204	0.0
28	1.1863916136349616E-4	0.0	0.0	0.012575751104530592	0.0
29	1.1863916136349616E-4	0.0	0.0	0.01969410078634036	0.0
30	1.1863916136349616E-4	0.0	0.0	0.03167665608405347	0.0
31	1.1863916136349616E-4	0.0	0.0	0.05481129254993522	0.0
32	1.1863916136349616E-4	0.0	0.0	0.0832846912771743	0.0
33	1.1863916136349616E-4	0.0	0.0	0.10748708019532752	0.0
34	1.1863916136349616E-4	0.0	0.0	0.13453680898620463	0.0
35	1.1863916136349616E-4	0.0	0.0	0.16395932100435168	0.0
36	1.1863916136349616E-4	0.0	0.0	0.20821172819293576	0.0
37	1.1863916136349616E-4	0.0	0.0	0.2706159270701347	0.0
38	1.1863916136349616E-4	0.0	0.0	0.355087009960944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	7.032348E-4	27.498665	5
ACCGAGT	50	0.002580523	21.998932	8
ATACCGT	135	1.1277734E-10	21.184156	6
CGTCTTA	85	6.1061037E-6	20.707335	15
TAGGACC	500	0.0	20.678995	4
GTCCTAA	330	0.0	20.668118	1
GTAGGAC	660	0.0	20.33114	3
GATATAC	455	0.0	20.309116	1
CTAGGAC	175	0.0	20.112116	3
ATAATAC	100	1.1237717E-6	19.797865	3
TAGGACG	585	0.0	19.554605	4
GCACTAC	80	8.9796195E-5	19.25135	1
TGTAGGA	710	0.0	18.89937	2
CGTATGC	70	8.119946E-4	18.857346	21
GCGCATG	70	8.126427E-4	18.855108	41
GTCTTAC	145	6.2937033E-9	18.208174	1
CTGTAGG	740	0.0	18.136408	1
AATCACG	365	0.0	18.08346	34
GTCCTAC	650	0.0	17.93972	1
AGGACGT	675	0.0	17.925056	5
>>END_MODULE