FastQCFastQC Report
Thu 2 Feb 2017
SRR4064040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160947
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18700.16107539792944897No Hit
TATCAACGCAGAGTACTTTTTTTTT13100.11283891512704715No Hit
GTCCTAAAGTGTGTATTTCTCATTT12680.10922117891686701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG250.006028015619.00172218
GGTATCA3650.014.8344061
GATTAGG400.005280897514.2488351
TCTAGTA400.00529299114.2439232
GTATCAA10300.012.7271161
CAAGGGT701.09171095E-412.2127634
CCCCCCT951.0373278E-612.00160716
TTATACT802.8685543E-511.8735174
TATACAC2000.011.8719833
GTGTAAG1459.640644E-1111.792141
GTCCTAC6300.011.6101631
TAAGACA1001.9308973E-611.3985774
GTATTAG855.3243566E-511.1755581
ATTATAC855.3337157E-511.1736313
TGCGATG600.00587016911.08433912
TACACCG600.00587770911.0824285
AGGGGTT803.771968E-410.6866265
TCTATTC909.533916E-510.5528743
TCCTACA6400.010.5345692
CCTACAC1451.4464604E-810.4800953
Produced by FastQC (version 0.11.5)