##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064040_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160947
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262209213685036	32.0	32.0	32.0	32.0	32.0
2	30.876866041257696	32.0	32.0	32.0	32.0	32.0
3	30.883769026493027	32.0	32.0	32.0	32.0	32.0
4	30.91518303591809	32.0	32.0	32.0	32.0	32.0
5	30.798772036966373	32.0	32.0	32.0	32.0	32.0
6	34.46864499412979	36.0	36.0	36.0	32.0	36.0
7	34.421469713949044	36.0	36.0	36.0	32.0	36.0
8	34.37593361281781	36.0	36.0	36.0	32.0	36.0
9	34.52917919594952	36.0	36.0	36.0	32.0	36.0
10	34.21294770562308	36.0	36.0	36.0	32.0	36.0
11	34.50374306492889	36.0	36.0	36.0	32.0	36.0
12	34.31620909481656	36.0	36.0	36.0	32.0	36.0
13	34.391959322863144	36.0	36.0	36.0	32.0	36.0
14	34.29545448672506	36.0	36.0	36.0	32.0	36.0
15	34.23021895056363	36.0	36.0	36.0	32.0	36.0
16	34.233256987614425	36.0	36.0	36.0	32.0	36.0
17	34.187754479748	36.0	36.0	36.0	32.0	36.0
18	34.16099873637642	36.0	36.0	36.0	32.0	36.0
19	34.17187175641954	36.0	36.0	36.0	32.0	36.0
20	34.156439527385835	36.0	36.0	36.0	32.0	36.0
21	34.15483049613807	36.0	36.0	36.0	32.0	36.0
22	34.12942365155343	36.0	36.0	36.0	32.0	36.0
23	34.07361404095105	36.0	36.0	36.0	32.0	36.0
24	34.060954548312715	36.0	36.0	36.0	32.0	36.0
25	33.64016875878055	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	6.0
5	12.0
6	47.0
7	16.0
8	77.0
9	68.0
10	105.0
11	29.0
12	70.0
13	66.0
14	100.0
15	220.0
16	310.0
17	409.0
18	474.0
19	649.0
20	913.0
21	1435.0
22	2245.0
23	3415.0
24	5459.0
25	8148.0
26	11834.0
27	16347.0
28	22566.0
29	31408.0
30	41892.0
31	57483.0
32	82227.0
33	117066.0
34	247138.0
35	508707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.26065789190237	17.31474978996575	11.902199435600268	26.522392882531616
2	16.13962487143396	19.422387460568775	38.51692075508105	25.921066912916213
3	18.92095130628031	23.190459373088817	28.90846131980395	28.980128000826923
4	12.964724042642631	15.70982613549441	35.90830938647812	35.41714043538484
5	14.42684325098226	36.62829512690347	34.433941540759605	14.51092008135467
6	34.00944867237883	35.667790606117514	16.944550214593754	13.378210506909902
7	30.182745848780417	30.272692888583702	20.86400851912193	18.68055274351395
8	27.61257368267314	34.411240867484494	19.27860128507924	18.697584164763118
9	27.35389295609571	14.372115670500229	18.42584014512675	39.84815122827731
10	15.693387250237869	27.555278616638397	32.84182421158593	23.909509921537804
11	36.25821864504503	21.22381862229266	23.08606512519402	19.43189760746829
12	24.257886131492555	23.483386526671797	29.855554875615503	22.40317246622015
13	30.10345549810422	20.252385561566925	24.787430540531922	24.856728399796932
14	23.110651410347575	19.491628418126574	25.71948197470209	31.678238196823756
15	24.789963043148504	28.023859922500932	22.12231269909125	25.063864335259318
16	24.749329635085317	25.995161133229157	24.72804000037925	24.527469231306277
17	23.696102420806856	26.421503267016195	25.722764306824576	24.159630005352373
18	23.857956847431623	25.815683536338323	26.92509934745317	23.401260268776884
19	25.591342237622992	25.2474970153821	25.264909082289254	23.89625166470565
20	25.623858375162385	25.318275738117833	25.412234856419264	23.645631030300525
21	25.420771659665657	24.72865884932905	24.980108976088534	24.870460514916758
22	25.46432863071112	25.267183995338183	25.268218422808435	24.000268951142267
23	24.737938263772033	25.339473393837412	25.440074066922293	24.48251427546826
24	24.939615504635082	24.908410383547146	25.667246518732554	24.48472759308522
25	24.766309220053788	25.175591338528374	25.62547410523412	24.432625336183712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	25.0
2	120.0
3	215.0
4	215.0
5	215.0
6	563.5
7	912.0
8	912.0
9	912.0
10	1237.5
11	1563.0
12	1563.0
13	1563.0
14	2371.5
15	3180.0
16	3180.0
17	3180.0
18	5766.5
19	8353.0
20	8353.0
21	8353.0
22	12769.5
23	17186.0
24	17186.0
25	17186.0
26	26152.0
27	35118.0
28	35118.0
29	35118.0
30	46333.0
31	57548.0
32	57548.0
33	57548.0
34	67505.0
35	77462.0
36	77462.0
37	77462.0
38	89492.0
39	101522.0
40	101522.0
41	101522.0
42	113679.5
43	125837.0
44	125837.0
45	125837.0
46	137938.0
47	150039.0
48	150039.0
49	150039.0
50	152415.0
51	154791.0
52	154791.0
53	154791.0
54	147299.5
55	139808.0
56	139808.0
57	139808.0
58	127194.5
59	114581.0
60	114581.0
61	114581.0
62	98227.5
63	81874.0
64	81874.0
65	81874.0
66	65276.0
67	48678.0
68	48678.0
69	48678.0
70	35930.0
71	23182.0
72	23182.0
73	23182.0
74	16891.0
75	10600.0
76	10600.0
77	10600.0
78	7597.0
79	4594.0
80	4594.0
81	4594.0
82	3324.0
83	2054.0
84	2054.0
85	2054.0
86	1380.0
87	706.0
88	706.0
89	706.0
90	504.0
91	302.0
92	302.0
93	302.0
94	221.0
95	140.0
96	140.0
97	140.0
98	408.5
99	677.0
100	677.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03634963525466709
2	0.005598877468135927
3	0.001464321799358627
4	0.003703872786612998
5	0.008958203949017482
6	0.012834349888496202
7	0.02274005617827515
8	0.03411008426741272
9	0.05013148748392476
10	0.05538581864632924
11	0.054093769999836346
12	0.06150151557306234
13	0.06356879340745097
14	0.06649743700616824
15	0.05814218909218078
16	0.0652915249361082
17	0.06150151557306234
18	0.07554177753161859
19	0.07166563159213986
20	0.07476654834372284
21	0.07545564095518573
22	0.07623087014308147
23	0.07881496743606728
24	0.07588632383735003
25	0.07468041176728997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1160947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.77526287903809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.41858025682455	24.82226810128068
2	17.035029243723137	14.23285649617271
3	8.29185361353693	10.391830933800213
4	4.688098903124659	7.83386256323851
5	2.9244631255500613	6.108510792495359
6	1.9404827992057085	4.863850742944209
7	1.3444664869634713	3.9315808647469086
8	0.9615944686473112	3.2136689368616302
9	0.6570432833883705	2.4703340297809944
>10	2.6433699174320298	17.36337985383849
>50	0.06501688960737581	1.849310832136875
>100	0.027931978349563402	2.0557574273425674
>500	0.0010345168233944749	0.29309913348012123
>1k	0.0010345168233944749	0.5696892918807075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1870	0.16107539792944897	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1310	0.11283891512704715	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1268	0.10922117891686701	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.613657643286042E-5
2	0.0	0.0	0.0	0.0	8.613657643286042E-5
3	0.0	0.0	0.0	0.0	8.613657643286042E-5
4	0.0	0.0	0.0	8.613657643286042E-5	8.613657643286042E-5
5	0.0	0.0	0.0	8.613657643286042E-5	1.7227315286572084E-4
6	0.0	0.0	0.0	8.613657643286042E-5	3.445463057314417E-4
7	0.0	0.0	0.0	8.613657643286042E-5	3.445463057314417E-4
8	0.0	0.0	0.0	8.613657643286042E-5	3.445463057314417E-4
9	0.0	0.0	0.0	1.7227315286572084E-4	3.445463057314417E-4
10	0.0	0.0	0.0	1.7227315286572084E-4	3.445463057314417E-4
11	0.0	0.0	0.0	1.7227315286572084E-4	3.445463057314417E-4
12	0.0	0.0	0.0	1.7227315286572084E-4	6.029560350300229E-4
13	0.0	0.0	0.0	1.7227315286572084E-4	7.752291878957438E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	25	0.0060280156	19.001722	18
GGTATCA	365	0.0	14.834406	1
GATTAGG	40	0.0052808975	14.248835	1
TCTAGTA	40	0.005292991	14.243923	2
GTATCAA	1030	0.0	12.727116	1
CAAGGGT	70	1.09171095E-4	12.212763	4
CCCCCCT	95	1.0373278E-6	12.001607	16
TTATACT	80	2.8685543E-5	11.873517	4
TATACAC	200	0.0	11.871983	3
GTGTAAG	145	9.640644E-11	11.79214	1
GTCCTAC	630	0.0	11.610163	1
TAAGACA	100	1.9308973E-6	11.398577	4
GTATTAG	85	5.3243566E-5	11.175558	1
ATTATAC	85	5.3337157E-5	11.173631	3
TGCGATG	60	0.005870169	11.084339	12
TACACCG	60	0.005877709	11.082428	5
AGGGGTT	80	3.771968E-4	10.686626	5
TCTATTC	90	9.533916E-5	10.552874	3
TCCTACA	640	0.0	10.534569	2
CCTACAC	145	1.4464604E-8	10.480095	3
>>END_MODULE