FastQCFastQC Report
Thu 2 Feb 2017
SRR4064040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160947
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.029.9412781
GTATCAA11150.026.836891
GACGTGA5450.022.6039777
TAGGACG8100.021.9985144
TTAGGAC6200.021.6437023
GTATTAG1301.6225385E-920.309821
GGACGTG9150.019.4740946
TTTAGGA6350.019.0538312
GTATAGG1658.185452E-1118.6686231
AGGACGT9450.018.623085
ACGACGC600.00741113318.33367521
CGTGAAA7000.018.2281259
GTCCTAC6150.017.8880521
TATACTG1852.1827873E-1117.8366345
TATCAAC16550.017.27982
CTTTAGG6950.017.0953161
GTAGGAC8050.016.9429553
GTCTTAA1307.308172E-716.924851
TAGGACT2750.016.7988664
TGAAATA8400.016.76149611
Produced by FastQC (version 0.11.5)