##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160947
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23061948564405	32.0	32.0	32.0	32.0	32.0
2	31.34918045354353	32.0	32.0	32.0	32.0	32.0
3	31.436531555704093	32.0	32.0	32.0	32.0	32.0
4	31.53111468482196	32.0	32.0	32.0	32.0	32.0
5	31.461038273065007	32.0	32.0	32.0	32.0	32.0
6	35.0623482381194	36.0	36.0	36.0	36.0	36.0
7	35.084530129282385	36.0	36.0	36.0	36.0	36.0
8	35.021922620067926	36.0	36.0	36.0	36.0	36.0
9	35.16770188475443	36.0	36.0	36.0	36.0	36.0
10	34.99421162206371	36.0	36.0	36.0	36.0	36.0
11	35.15106288228489	36.0	36.0	36.0	36.0	36.0
12	35.06502450154917	36.0	36.0	36.0	36.0	36.0
13	35.10409432988758	36.0	36.0	36.0	36.0	36.0
14	35.045996070449384	36.0	36.0	36.0	36.0	36.0
15	35.0119359453963	36.0	36.0	36.0	36.0	36.0
16	35.0428589763357	36.0	36.0	36.0	36.0	36.0
17	35.013383039880374	36.0	36.0	36.0	36.0	36.0
18	35.008060660822586	36.0	36.0	36.0	36.0	36.0
19	35.00924331601701	36.0	36.0	36.0	36.0	36.0
20	34.998801840221816	36.0	36.0	36.0	36.0	36.0
21	34.99472241196196	36.0	36.0	36.0	36.0	36.0
22	34.97102882388257	36.0	36.0	36.0	36.0	36.0
23	34.937636257296845	36.0	36.0	36.0	36.0	36.0
24	34.91707717923385	36.0	36.0	36.0	36.0	36.0
25	34.88935929030352	36.0	36.0	36.0	32.0	36.0
26	34.82862439026071	36.0	36.0	36.0	32.0	36.0
27	34.81788832737412	36.0	36.0	36.0	32.0	36.0
28	34.79629302629664	36.0	36.0	36.0	32.0	36.0
29	34.77797780604972	36.0	36.0	36.0	32.0	36.0
30	34.75027456033738	36.0	36.0	36.0	32.0	36.0
31	34.74865433133468	36.0	36.0	36.0	32.0	36.0
32	34.711001449678584	36.0	36.0	36.0	32.0	36.0
33	34.70420441243226	36.0	36.0	36.0	32.0	36.0
34	34.696812171442794	36.0	36.0	36.0	32.0	36.0
35	34.67036651974638	36.0	36.0	36.0	32.0	36.0
36	34.64517415523706	36.0	36.0	36.0	32.0	36.0
37	34.62430584686467	36.0	36.0	36.0	32.0	36.0
38	34.596657728561254	36.0	36.0	36.0	32.0	36.0
39	34.57811338502102	36.0	36.0	36.0	32.0	36.0
40	34.574903936183134	36.0	36.0	36.0	32.0	36.0
41	34.55938815467028	36.0	36.0	36.0	32.0	36.0
42	34.519534483486325	36.0	36.0	36.0	32.0	36.0
43	34.50199104696424	36.0	36.0	36.0	32.0	36.0
44	34.45859630112314	36.0	36.0	36.0	32.0	36.0
45	34.43249519573245	36.0	36.0	36.0	32.0	36.0
46	34.409510511677105	36.0	36.0	36.0	32.0	36.0
47	34.38756032790472	36.0	36.0	36.0	32.0	36.0
48	34.3500624920862	36.0	36.0	36.0	32.0	36.0
49	34.3343813283466	36.0	36.0	36.0	32.0	36.0
50	33.73980552083773	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	1.0
19	3.0
20	11.0
21	25.0
22	96.0
23	307.0
24	841.0
25	1940.0
26	3913.0
27	7273.0
28	12301.0
29	19316.0
30	28360.0
31	40874.0
32	60020.0
33	98069.0
34	204882.0
35	682712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33479493121301	18.37306065487625	12.421112479863545	26.871031934047192
2	15.459541406101952	20.191568901062933	37.89377573345737	26.455113959377748
3	18.17459052754437	23.7341131578154	29.272446153779896	28.818850160860332
4	12.542605304118105	16.527541739631523	36.070897293330354	34.85895566292001
5	14.12131647697957	37.04544651909174	34.05056389309762	14.782673110831071
6	34.14258925718144	36.056722853749065	16.638284566942563	13.162403322126925
7	30.027382817648007	30.48752440895235	20.825153947596228	18.65993882580342
8	27.56568559977329	34.715107580277135	19.08062986510151	18.63857695484807
9	27.30996202212175	14.226501133995843	18.349629050277404	40.113907793605
10	15.584313786292695	27.791286910726882	32.948646057558385	23.67575324542204
11	36.56626874439574	21.03601628670387	22.92016775959626	19.477547209304127
12	24.221777566073214	23.537077920008407	29.84787419236192	22.393270321556454
13	30.17872632192789	20.16500464710833	24.88016145578959	24.77610757517419
14	23.17758377873198	19.432703530313667	25.64712367941634	31.742589011538016
15	24.914440865781774	28.10586333107358	21.9809233064546	24.998772496690048
16	24.736742820669853	26.066530741978244	24.681356524944935	24.51536991240696
17	23.84096776166354	26.550307636782726	25.473686567948405	24.135038033605323
18	24.01939799043021	25.866427780816494	26.809194234007638	23.304979994745658
19	25.62126059265461	25.30177975547528	25.287394934820394	23.789564717049714
20	25.826032502943736	25.230567272583187	25.23961165985179	23.703788564621288
21	25.523904192008768	24.69234168312593	24.98856536947854	24.795188755386764
22	25.54845500597359	25.252574846482556	25.295384872470052	23.903585275073798
23	24.896936787321977	25.27499315208909	25.385076550168918	24.44299351042002
24	25.058616192712467	25.098153051573636	25.39239686392602	24.450833891787877
25	24.84247502211595	25.198397487525053	25.563019897030564	24.396107593328434
26	24.171749017986354	25.580421749017983	25.9012128729929	24.346616360002756
27	24.62270002067397	25.677072565639858	25.320274274688163	24.379953138998
28	24.60513122667742	25.438886649231797	25.317514463016373	24.638467661074415
29	24.780087448187466	25.291752374848826	24.923939372013134	25.00422080495057
30	24.498234128693255	25.406322680678784	25.2318890515979	24.863554139030065
31	25.06085973747185	24.77478020719015	25.408100504278703	24.756259551059294
32	24.498988285822822	25.73992097517359	25.318868706289337	24.442222032714245
33	24.652765813756368	25.551905093342338	25.493070821646878	24.302258271254416
34	24.948402457386802	25.183218652985424	25.7242606649278	24.144118224699977
35	24.494422866377064	25.559802257040005	25.582973914226965	24.362800962355962
36	24.359868924311026	25.325707925875392	25.73230443741515	24.582118712398437
37	24.790421224911707	25.18296149539151	25.300284262210354	24.726333017486432
38	24.808676326171977	25.614458246472445	25.150319590648312	24.42654583670727
39	25.09133039594451	24.918618824269206	25.390237704529696	24.59981307525659
40	25.27263142298959	24.95632728863958	25.38108101589446	24.389960272476372
41	24.800824181740346	24.819602570055014	25.827605781331698	24.55196746687294
42	24.72515403691718	25.344833378268845	25.544502090147237	24.385510494666743
43	24.593817026005013	25.171112814726126	25.79328641692436	24.4417837423445
44	24.235786419876444	25.231796522104656	25.59684804967044	24.935569008348466
45	24.2236800622951	25.213924057175046	25.74634386687868	24.81605201365117
46	24.661005707473464	24.811660266647372	25.45459087252161	25.072743153357557
47	24.49032153587475	25.071322252542792	25.791866506794562	24.646489704787893
48	24.253551675336432	25.23552218004343	25.488508791650837	25.0224173529693
49	24.521810736453673	25.012877473637786	25.65683729247456	24.80847449743398
50	23.99937980756807	25.73876116561722	25.482501098257433	24.77935792855728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	10.5
2	20.0
3	65.0
4	110.0
5	120.0
6	130.0
7	145.0
8	160.0
9	184.5
10	209.0
11	354.0
12	499.0
13	884.0
14	1269.0
15	1660.0
16	2051.0
17	1988.0
18	1925.0
19	1986.0
20	2047.0
21	2500.0
22	2953.0
23	3779.5
24	4606.0
25	5848.0
26	7090.0
27	9188.5
28	11287.0
29	14433.5
30	17580.0
31	20982.0
32	24384.0
33	28666.5
34	32949.0
35	38463.0
36	43977.0
37	45968.5
38	47960.0
39	51069.5
40	54179.0
41	56568.5
42	58958.0
43	61443.0
44	63928.0
45	68484.5
46	73041.0
47	76671.0
48	80301.0
49	83453.5
50	86606.0
51	88116.0
52	89626.0
53	88895.5
54	88165.0
55	85509.0
56	82853.0
57	78639.5
58	74426.0
59	68740.5
60	63055.0
61	55266.0
62	47477.0
63	41180.0
64	34883.0
65	28966.0
66	23049.0
67	19027.5
68	15006.0
69	12217.0
70	9428.0
71	7337.0
72	5246.0
73	4485.0
74	3724.0
75	3003.0
76	2282.0
77	1838.5
78	1395.0
79	1143.5
80	892.0
81	731.5
82	571.0
83	440.0
84	309.0
85	231.0
86	153.0
87	120.5
88	88.0
89	75.5
90	63.0
91	36.0
92	9.0
93	9.5
94	10.0
95	10.0
96	10.0
97	6.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010077979442644668
2	6.029560350300229E-4
3	1.7227315286572084E-4
4	0.0
5	0.0
6	3.445463057314417E-4
7	0.0
8	0.0
9	6.890926114628833E-4
10	3.445463057314417E-4
11	0.0
12	0.0
13	8.613657643286042E-4
14	1.7227315286572084E-4
15	0.004651375127374463
16	8.613657643286042E-4
17	0.0
18	1.7227315286572084E-4
19	8.613657643286042E-5
20	5.168194585971625E-4
21	0.0
22	3.445463057314417E-4
23	7.752291878957437E-4
24	4.306828821643021E-4
25	0.0017227315286572084
26	0.005771150621001648
27	0.005771150621001648
28	0.004737511703807323
29	0.002670233869418673
30	0.0040484190923444395
31	0.006977062691061694
32	0.003962282515911578
33	0.005082058009538764
34	0.0033593264808815565
35	0.003962282515911578
36	0.007838428455390298
37	0.0040484190923444395
38	0.007493882149658856
39	0.004479101974508741
40	0.0033593264808815565
41	0.0032731899044486958
42	0.00206727783438865
43	0.001464321799358627
44	0.0012920486464929062
45	0.0018088681050900685
46	0.0011197754936271853
47	0.0016365949522243479
48	0.0013781852229257667
49	4.306828821643021E-4
50	0.001464321799358627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1160947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.87808903888098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.96494359300608	27.80868255856165
2	16.13168589875094	14.479184722228112
3	7.826305385045705	10.536888897466646
4	4.388737766914376	7.8783265708753225
5	2.755042129179629	6.182051298959582
6	1.8106367667104866	4.875475082010288
7	1.2181011252182754	3.826623552913481
8	0.857446405167778	3.078444489375037
9	0.6426642067648691	2.595738734396605
>10	2.343966203478372	16.261628434933
>50	0.04637842497523267	1.4099751953023463
>100	0.013899052724337368	1.0153468437461324
>500	1.9304206389045463E-4	0.051633619231890034
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7227315286572084E-4	0.0
5	0.0	0.0	0.0	1.7227315286572084E-4	0.0
6	0.0	0.0	0.0	1.7227315286572084E-4	0.0
7	0.0	0.0	0.0	1.7227315286572084E-4	0.0
8	0.0	0.0	0.0	1.7227315286572084E-4	0.0
9	0.0	0.0	0.0	2.5840972929858124E-4	0.0
10	0.0	0.0	0.0	2.5840972929858124E-4	0.0
11	0.0	0.0	0.0	2.5840972929858124E-4	0.0
12	0.0	0.0	0.0	2.5840972929858124E-4	4.3068288216430205E-4
13	0.0	0.0	0.0	2.5840972929858124E-4	5.168194585971625E-4
14	0.0	0.0	0.0	2.5840972929858124E-4	5.168194585971625E-4
15	0.0	0.0	0.0	2.5840972929858124E-4	9.475023407614645E-4
16	0.0	0.0	0.0	2.5840972929858124E-4	9.475023407614645E-4
17	0.0	0.0	0.0	2.5840972929858124E-4	9.475023407614645E-4
18	0.0	0.0	0.0	2.5840972929858124E-4	9.475023407614645E-4
19	0.0	0.0	0.0	4.3068288216430205E-4	9.475023407614645E-4
20	0.0	0.0	0.0	4.3068288216430205E-4	0.0012059120700600458
21	0.0	0.0	0.0	4.3068288216430205E-4	0.0012059120700600458
22	0.0	0.0	0.0	4.3068288216430205E-4	0.0012059120700600458
23	0.0	0.0	0.0	5.168194585971625E-4	0.0012059120700600458
24	0.0	0.0	0.0	7.752291878957438E-4	0.0012059120700600458
25	0.0	0.0	0.0	9.475023407614645E-4	0.0012059120700600458
26	0.0	0.0	0.0	0.0011197754936271853	0.0012920486464929062
27	0.0	0.0	0.0	0.0017227315286572082	0.0012920486464929062
28	0.0	0.0	0.0	0.003359326480881556	0.0012920486464929062
29	0.0	0.0	0.0	0.008872067372584622	0.0012920486464929062
30	0.0	0.0	0.0	0.016193676369377758	0.0012920486464929062
31	0.0	0.0	0.0	0.04255146875783305	0.0012920486464929062
32	0.0	0.0	0.0	0.07037358294564695	0.0012920486464929062
33	0.0	0.0	0.0	0.09604228272263936	0.0013781852229257667
34	0.0	0.0	0.0	0.1294632743785892	0.0015504583757914876
35	0.0	0.0	0.0	0.16486540729249483	0.0015504583757914876
36	0.0	0.0	0.0	0.22033736251525693	0.0015504583757914876
37	0.0	0.0	0.0	0.3094887191232675	0.0015504583757914876
38	0.0	0.0	0.0	0.43921040323115523	0.0015504583757914876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	485	0.0	29.941278	1
GTATCAA	1115	0.0	26.83689	1
GACGTGA	545	0.0	22.603977	7
TAGGACG	810	0.0	21.998514	4
TTAGGAC	620	0.0	21.643702	3
GTATTAG	130	1.6225385E-9	20.30982	1
GGACGTG	915	0.0	19.474094	6
TTTAGGA	635	0.0	19.053831	2
GTATAGG	165	8.185452E-11	18.668623	1
AGGACGT	945	0.0	18.62308	5
ACGACGC	60	0.007411133	18.333675	21
CGTGAAA	700	0.0	18.228125	9
GTCCTAC	615	0.0	17.888052	1
TATACTG	185	2.1827873E-11	17.836634	5
TATCAAC	1655	0.0	17.2798	2
CTTTAGG	695	0.0	17.095316	1
GTAGGAC	805	0.0	16.942955	3
GTCTTAA	130	7.308172E-7	16.92485	1
TAGGACT	275	0.0	16.798866	4
TGAAATA	840	0.0	16.761496	11
>>END_MODULE