FastQCFastQC Report
Thu 2 Feb 2017
SRR4064039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498673
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31220.20831762499224313No Hit
TATCAACGCAGAGTACTTTTTTTTT21380.14265953947258675No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16540.1103643022860891No Hit
GATTAAGAGGGACGGCCGGGGGCAT15960.10649421187944266No Hit
GAATAGGACCGCGGTTCTATTTTGT15600.10409208679945527No Hit
GCGCAAGACGGACCAGAGCGAAAGC15330.10229049298946467No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15260.10182341311280045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC250.006028732819.001777
TCGGTTG350.002167349416.28886217
TATAGCG400.005285081514.2475215
GCGTTAT1203.6379788E-1214.2451431
CGTTATT1203.6379788E-1214.2437172
AGGCCCG3250.013.44695810
CGCGGTA852.6946327E-713.41346318
ATCGCCA2500.013.30257216
CGACCAT3100.013.17820710
CGAGCCG3350.012.76366115
CGATAAC1501.8189894E-1212.66742310
CCGACCA3250.012.5704029
CGCATCG2450.012.40973313
CGTCGTA2150.012.37283210
CGGTCCA3000.012.35073910
TATTAGC1551.8189894E-1212.252662
CGAACGA1405.2750693E-1112.21664716
TATTCCC2200.012.0888065
AATACTA802.8713052E-511.8729345
GTTATTC1603.6379788E-1211.8697643
Produced by FastQC (version 0.11.5)